2018 Fiscal Year Annual Research Report
Identification of novel testis-specific long noncoding RNAs: a new molecular basis of infertility
| Project/Area Number |
17K16818
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Keywords | testis-specific lncRNAs / spermatogenesis / mouse / long-non-coding RNAs |
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| Outline of Annual Research Achievements |
Long non-protein-coding RNAs (lncRNAs) are key regulators in gene expression of specific tissues. The purpose of my research is to discover testis-specific lncRNAs expression and functions in mouse spermatogenesis. I reported about the expression pattern of two testis-specific lncRNAs (1700108J01Rik and 17001011022Rik) during spermatogenesis by ISH analyses. Then, I performed in vivo functional analysis: GFP-tagged viral microinjection in PD8-10 testis and transfection analyses in spermatogenesis. As results, I found GFP transfection mainly in the Sertoli cell nuclei and cytoplasmic processes, suggesting that viral-mediated GFP was not easily transfected to spermatogenetic cells. Therefore, I am now trying to perform in vitro functional analysis using target lncRNAs induced COS-7 cells.
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