2018 Fiscal Year Final Research Report
Identification of novel testis-specific long noncoding RNAs: a new molecular basis of infertility
| Project/Area Number |
17K16818
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Keywords | long non-coding RNAs / testis-specific lncRNA / spermatogenesis / testis |
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| Outline of Final Research Achievements |
Long non-protein-coding RNAs (lncRNAs) are key regulators in gene expression. However, lncRNAs associated testis-specific expression remains functionally undetermined. Firstly, I have discovered candidates for testis-specific lncRNAs. The aim of this research is (1) To characterize the newly identify testis-specific lncRNAs in mouse and (2) to perform functional analysis of the testis-specific lncRNAs. As results, the antisense lncRNA 1700108J01Rik and long intergenic non-coding RNA 1700101O22Rik were identified as testis-specific lncRNAs. These lncRNAs showed different stage-specific and cell-type specific expression pattern during mouse spermatogenesis. Next, I tried to do functional analysis of these lncRNAs by in vivo lentiviral-mediated transfection and target gene manipulation (still in under investigation).
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| Free Research Field |
biology reproduction
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| Academic Significance and Societal Importance of the Research Achievements |
This study provides useful information about testis-specific lncRNAs in mouse spermatogenesis. It would apply (1) for future investigation of testis-specific lncRNA involved in spermatogenesis and testicular functions, and (2) for better understanding of a new molecular basis of male reproduction.
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