2019 Fiscal Year Annual Research Report
Probing the effect of degenerated retina potentiation on the synaptogenesis after iPSC-derived retinal transplantation
| Project/Area Number |
17K16994
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Tu HungYa 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (10780835)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | retinal organoid / transplantation / retinal degeneration / amarine cell / electrophysiology / MEA / ganglion cell |
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| Outline of Annual Research Achievements |
The numerous residual retinal ganglion cells in deafferented retinas are found exhibiting rhythmic membrane potential oscillation and spontaneous spiking activity. These oscillatory activities originate from the intrinsic membrane property of AII amacrine cells via transmission of cone bipolar cells to downstream neurons, and it is of great interest to clarify if these activities affect the reconstruction of retinal circuitry. The effect of pharmacological suppression of inner retinal hyperactivity on retinal reconstruction after stem cell-derived retinal transplantation is therefore probed here. By MEA recording, the systematic functional evaluation of transplanted retinas is conducted to compare the light responsiveness in transplanted mice treated with or without AII activity blockade.
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