2019 Fiscal Year Final Research Report
Probing the effect of degenerated retina potentiation on the synaptogenesis after iPSC-derived retinal transplantation
| Project/Area Number |
17K16994
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Tu Hung-Ya 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (10780835)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | transplantation / organoid / retinal degeneration / amacrine cell / electrophysiology / ganglion cell |
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| Outline of Final Research Achievements |
While fundamental properties of the normal retinal wiring are largely revealed in the past several decades, other counterintuitive but intriguing properties have recently been explored from diseased retinal models. The inner retinal hyperactivity has been commonly seen in deafferented retinas, in which many of the residual retinal ganglion cells are found exhibiting rhythmic membrane potential oscillation and spontaneous spiking activity. However, whether the inner retinal hyperactivity would affect the reconstruction of retinal circuitry remains unclear. The present study is therefore aimed to probe the effect of pharmacological suppression of inner retinal hyperactivity on retinal reconstruction after stem cell-derived retinal transplantation. By MEA recording, the systematic functional evaluation method of transplanted retinas has been established and used to compare the light responsiveness in transplanted mice treated with or without inner retinal hyperactivity blockade.
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| Free Research Field |
retinal neurobiology
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| Academic Significance and Societal Importance of the Research Achievements |
Although the present study is still preliminary with much room for improvement technically, the results indicate the potential of host retina manipulation to optimize retinal transplantation therapy, and lead to the idea for a more thorough investigation in the following KAKENHI study.
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