2019 Fiscal Year Final Research Report
An investigation into the formation processes of collagen bundles in periodontal ligament by innovative imaging methods
| Project/Area Number |
17K17090
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Kurume University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | 歯根膜 / FIB-SEM / 3次元解析 / コラーゲン線維 / ボリュームイメージング / 定量解析 / 細胞性ネットワーク / 異種細胞間ネットワーク |
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| Outline of Final Research Achievements |
The periodontal ligament (PDL) has important functions besides tooth anchoring including proprioception, sensory detection, and repair of damaged tissue. However, little is known about how the regular-PDL fiber bundle arrays are formed, maintained, and remodeled over large distances from cementum to alveolar bone. We investigated the three-dimensional ultrastructureof PDL fibroblasts (PDLFs) and PDL collagen bundles by FIB-SEM tomography. We have shown that PDLFs have a flat shape with long processes or a wing-like shape, not spindle shape, while PDL bundles are a multiple-branched structure wrapped in thin sheets of PDLF cytoplasm. Furthermore, PDLFs form an extensive cellular network between the cementum and alveolar bone. Our previous histomorphological study revealed the osteocyte-osteoblast-PDL cellular network. We extended this knowledge and revealed the cementum-PDL-bone cellular network, which may orchestrate the remodeling and modification of periodontal tissue.
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| Free Research Field |
解剖学
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| Academic Significance and Societal Importance of the Research Achievements |
歯根膜は歯の周囲に存在し、コラーゲン線維（歯根膜線維）を主体とする組織であり、歯の機能に必須である。また、臨床的にも治療の予後に関わる重要な組織である。しかしながら、歯-骨間にわたる長い歯根膜線維の形成過程、線維の配向性はどのように決められるのか、力学的負荷による歯根膜線維の改変過程など、未だ不明な点が多い。本研究は、これまで技術的な問題から困難であったメゾスケールでの歯根膜細胞と歯根膜線維束の3次元微細構造を明らかにした。また、セメント質-歯根膜-骨における異種細胞間ネットワークの存在を形態学的に示した。本研究の結果は今後、歯の再生治療を検討していく上で、基盤的知見になり得ると考えられる。
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