2018 Fiscal Year Annual Research Report
Elucidation of the functions of Ywhag and Ywhae in bone development using their knockout mice
| Project/Area Number |
17K17094
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| Research Institution | Nagasaki University |
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Principal Investigator |
姜 晴 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (00790007)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Keywords | Ywhag / Ywhae / survival rate / dwarfism |
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| Outline of Annual Research Achievements |
Wild-type and Ywhag-/-, Ywhae-/- samples were collected at E15.5 and E18.5. We stained whole skeletons with alcian blue and alizarin red to identify cartilage and calcified tissues, respectively. Ywhag-/- showed similar level compared with wild-type, while Ywhae-/- embryos showed mild dwarfism, size of Ywhae-/- skeletons was smaller than wild-type.
Due to embryonic lethality of Ywhae-/- mice, wild-type and Ywhag-/-, Ywhae+/- samples were obtained from the mice at 4 weeks and 8 weeks of age. Body weight and bone volume of Ywhag-/- mice and Ywhae+/- mice were lower than that of wild-type mice, especially at 4-6 weeks of age.
The survival rate of Ywhag-/- mice at 8 weeks of age was lower than Ywhag+/+ and Ywhag+/- mice. Survival rate of Ywhae+/- mice was lower than that of wild-type.
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