Elucidation of the functions of Ywhag and Ywhae in bone development using their knockout mice

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K17094
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: JIANG Qing 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (00790007)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Keywords: 骨代謝学

Outline of Final Research Achievements

Wild-type and Ywhag-/-, Ywhae-/- samples were collected at E15.5 and E18.5. We stained whole skeletons with alcian blue and alizarin red to identify cartilage and calcified tissues, respectively. Ywhag-/- showed similar level compared with wild-type, while Ywhae-/- embryos showed mild dwarfism, size of Ywhae-/- skeletons was smaller than wild-type.
Due to embryonic lethality of Ywhae-/- mice, wild-type and Ywhag-/-, Ywhae+/- samples were obtained from the mice at 3 weeks and 8 weeks of age. Body weight and bone volume of Ywhag-/- mice and Ywhae+/- mice were lower than that of wild-type mice, especially at 3-6 weeks of age.
The survival rate of Ywhag-/- mice at 8 weeks of age was lower than Ywhag+/+ and Ywhag+/- mice. Survival rate of Ywhae+/- mice was lower than that of wild-type.

Free Research Field

医歯薬学

Academic Significance and Societal Importance of the Research Achievements

Elucidation of the molecular mechanisms of 14-3-3s will hopefully provide novel insights into the therapy of many diseases, such as osteoporosis, and is useful for new drugs development for osteoporosis and bone regeneration.