2020 Fiscal Year Final Research Report
Mechanism of non-infectious pulpitis caused by HMGB1,endogenous protein, and its clinical application
Project/Area Number |
17K17118
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Conservative dentistry
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
Yamamoto Mioko 東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (50732749)
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Project Period (FY) |
2017-04-01 – 2021-03-31
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Keywords | HMGB1 |
Outline of Final Research Achievements |
To investigate the involvement of a nuclear protein called high mobility group box 1 (HMGB1), which is involved in non-infectious inflammatory diseases, in pulp inflammation, necrotic cell supernatants were prepared using mouse dental papilla cells (MDP) and added to MDP. The results showed that MDP inhibited cell proliferation, increased gene expression of the pro-inflammatory cytokine Interleukin-6 (IL6) and IL6 protein production, and also released HMGB1 protein. In addition, when HMGB1 protein was added to MDP, gene expression of IL6 and HMGB1 was increased. This suggests that HMGB1 in NCS may be involved in the inflammation of dental pulp.
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Free Research Field |
歯内治療
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Academic Significance and Societal Importance of the Research Achievements |
歯を長期にわたって口腔内で保存するためには歯髄の保存が重要である。歯髄炎・歯髄壊死は歯髄を失う原因である。通常の歯髄炎はう蝕によっておこる。しかし、う蝕のない歯においても歯髄炎や歯髄壊死を認めることがあり非感染性の関与が疑われる。High mobility group box 1(HMGB1)と呼ばれる核内タンパク質はダメージを受けた細胞から放出され炎症を修飾する。この研究ではHMGB1と歯髄細胞の炎症との関連について調べた。この研究は歯髄における非感染性の炎症のメカニズムの一端を解明する一助となり将来歯髄炎を抑制する治療戦略の創生につながることが期待できる。
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