2017 Fiscal Year Research-status Report
Dentin regeneration by dental pulp stem cells using nephronectin
| Project/Area Number |
17K17139
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
唐 佳 北海道医療大学, 歯学部, 助教 (80755992)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Keywords | Nephronectin / dentin regeneration / iMatrix411 / iMatrix511 |
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| Outline of Annual Research Achievements |
The purpose of this study was to investigate the effects of nephronectin (Npnt) in human dental pulp stem cells (hDPSCs). The results found Npnt mediates hDPSCs adhesion and spreading partially via RGD motif. Npnt enhanced the mRNA expression of ITGA1, ITGA4, ITGA7 and ITGB1 on day five. Npnt downregulated DSPP but significantly upregulated BSP at day 28. Moreover, Npnt and FCOL1 accelerated the matrix mineralization in hDPSCs. The findings implicated Npnt would be favorable to recruit hDPSCs and conducive to mineralization in hDPSCs. The combination of Npnt with hDPSCs may offer a promising approach for hard tissue regeneration.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The hard tissue forming capacity of Npnt in hDPSCs has already been demonstrated by our research results.
It is thus believed our research is progressing smoothly.
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| Strategy for Future Research Activity |
In the future work, we intend to synthesize several artificial RGD-containing peptides derived from Npnt amino acid sequence and compare the hard tissue inducing capacities among them, in an effort to determine the most promising one to be applied in the in vivo animal study.
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| Causes of Carryover |
It is hard to use exactly the same amount that was allocated in the first fiscal year, the PI plans to use the remaining 3,086 together with the amount allocated for the next fiscal year (2018-2019) for procurement of experiment chemicals.
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