Trial of jaw bone regeneration based on Notch signal regulation of iPS cells

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K17148
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Tohoku University
• Principal Investigator: Kamano Yuya 東北大学, 大学病院, 助教 (70757260)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Keywords: iPS細胞 / Notchシグナル / 骨芽細胞分化

Outline of Final Research Achievements

We succeeded in establishing NICD1-miPS cells, which can regulate Notch signal, which is important for neural crest cell differentiation, in a doxycycline concentration-dependent manner. This enabled us to examine the role of Notch signaling in osteoblast differentiation of iPS cells.
Moreover, as a result of performing osteoblast differentiation using the established cell line, in the group in which the Notch signal was constitutively expressed, osteoblast differentiation was slightly suppressed. Further studies are planned in the future to clarify the suppression mechanism.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

現在までにおいて、iPS細胞の骨芽細胞分化におけるNotchシグナルの役割は十分に分かっていない。今回樹立した細胞を用いることにより、iPS細胞の骨芽細胞分化におけるNotchシグナルの解明が容易になると考えられる。また、Notchシグナルは神経堤細胞分化に重要とされており、顎骨は神経堤細胞由来の骨であることから、iPS細胞を用いた顎骨再生のメカニズム解明に役立つと考えられる。