Scx regulates differentiation of PDL cells into osteoblasts under tensile force loading

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K17305
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: Kawatsu Masayoshi 東北大学, 歯学研究科, 大学院非常勤講師 (70712925)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: scleraxis / 歯根膜細胞 / 機械的刺激

Outline of Final Research Achievements

I examined effect of Scx on tensile force-induced osteoblast differentiation and its underlying mechanisms. In the mouse experimental tooth movement model, expression of Scx,TGF-β1, p-Smad3 increased on the tension side PDL. I loaded tensile force onto cultured PDL cells. The in vitro data showed TGF signaling regulated tensile force-induced Scx expression. Scx knockdown in PDL cells upregulated ALP expression, while inhibited ephrin A2 expression.

Free Research Field

矯正歯科

Academic Significance and Societal Importance of the Research Achievements

本研究により、メカニカルストレスが負荷された環境におけるScxによるPDL細胞の骨芽細胞分化の抑制的制御と、その分子メカニズムが明らかになった。これらの成果は、歯根膜細胞シートを用いた歯周組織再生治療方法の開発へとつながる基盤的知見となり得る。