2018 Fiscal Year Annual Research Report
Isothermal DNA Sequencing by Diffusion Current in a MoS2 Nanopore
| Project/Area Number |
17K17682
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| Research Institution | The University of Tokyo |
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Principal Investigator |
徐 偉倫 東京大学, 大学院工学系研究科(工学部), 助教 (50771549)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Keywords | DNA sequencing / Two-dimensional material / Biosensing / Diffusiophoresis / Diffusion current / Nanopore |
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| Outline of Annual Research Achievements |
We have proposed a new method for DNA sequencing using solid-state nanopores. Prior to this research, no structural information of a DNA molecule had ever been read for solid-state nanopore resistive pulse sensing using an applied external electric field, mainly due to (i) fast DNA translocation speed, (ii) high temperature fluctuation and (iii) insufficient spatial resolution. These problems have been successfully resolved using our new method by replacing the electric field with a solute concentration gradient that provides diffusion current for isothermal DNA structure detection. We have obtained multi-levels of current blockage signals of dsDNA oligonucleotides indicating various nucleotide bases (first time in history). The results revive hopes for solid-state nanopore DNA sequencing.
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| Remarks |
Based on the research outcome of this project, the principal investigator received the Young Scientist Award of the year 2018 from International Association of Advanced Materials in Stockholm, Sweden.
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