Study for screening enzymes involved in biosynthesis of ornithine-derived alkaloids, and microbial production

2019 Fiscal Year Final Research Report

• Project/Area Number: 17K18002
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bioorganic chemistry; Applied microbiology
• Research Institution: University of Shizuoka
• Principal Investigator: Hasebe Fumihito 静岡県立大学, 食品栄養科学部, 助教 (30781801)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: アルカロイド / 微生物生産 / トロピノン

Outline of Final Research Achievements

In this study, we attained the microbial production of plant-specific alkaloids, Tropinone and N-Methyl-Δ1-pyrrolinium cation(N-mpyr), by co-expressing Putrescine N-methyltransferase (PMT) from Nicotiana tabacum, Putrescine aminotransferase (YgjG) from Escherichia coli, CHSB from Solanum lycopersicum (SlCHSB), CYP82M3 from Atropa belladonna L., and CYP reductase from Lotus japonicus. We also achieved the increased production of Tropinone and N-mpyr by using E. coli, which disrupted two genes, puuC and ydcW, as a host. Furthermore, we revealed that SlCHSB synthesizes 3-oxoglutaric acid and does not recognize N-mpyr.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

本研究は、植物に特異な代謝産物であり、医薬品として利用されているスコポラミンやアトロピンといったトロパンアルカロイドの重要な鍵中間体であるトロピノンやN-メチル-Δ1-ピロリニウムカチオン(N-mpyr)の微生物生産を達成した。本研究成果は、植物の生育に非依存的なトロパンアルカロイドの安価大量生産の実現に寄与すると考えている。