2017 Fiscal Year Research-status Report
Pre-emptive Delivery of Pain Specific Local Anaesthetic (QX-CAP) to Prevent Endodontic Postoperative Pain
| Project/Area Number |
17K18209
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | QX314 / Capsaicin / Endodontic pain / Trigeminal nerve / c-Fos |
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| Outline of Annual Research Achievements |
The aim of this study was to investigate the possibility of using the combination of QX314 & capsaicin (QX-CAP) in preventing postoperative neuropathic like pain after endodontic treatment. Therefore we need to establish a model of endodontic treatment in rats. In the last year, in a pilot study, we tried to establish a method to make a rat model with endodontic treatment procedure. We tried with pulp exposure of the rat`s lower central incisor using dental dril and followed by repeated stimulation of dental pulp using a endodontic file to mimic the endodontic procedure. We checked the c-Fos expression in the spinal trigeminal sub-nucleus caudalis (Vc). The c-Fos expression was increased in the trigeminal sub-nucleus caudalis, two hours following the repeated stimulation of dental pulp using a endodontic file that mimicked the endodontic procedure. We than investigated whether prior infiltration ofthe combination of QX314 & capsaicin (QX-CAP), in the oral mucosa labial side of the incisor reduced the c-Fos expression that was increased due to the endodontic procedure. We observed that pre-emptive delivery of the QX-CAP reduced the increased c-Fos expression in the spinal trigeminal sub-nucleus caudalis.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
A rat model was prepared to mimic the endodontic procedure. In the pilot study, c-Fos expression increased by the mimicked endodontic procedure as expected. The preemptive delivery of the combination of QX-CAP reduced the increased c-Fos expression in the spinal trigeminal sub-nucleus caudalis in the pilot study.
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| Strategy for Future Research Activity |
We need to elaborate the results of the pilot study by increasing the number of rats and investigate that how much c-Fos expression in the Vc will be reduced by preemptive delivery of the QX-CAP. We will check the c-Fos expression in the Vc following pre-emptive delivery of QX314 only. We will also check the c-Fos expression in the Vc following pre-emptive delivery of capsaicin only. We also need to investigate by doing same procedure in other groups of rats, like sham operated group, naive group, following pre-emptive delivery of lidocaine or saline.
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| Causes of Carryover |
The small remaining ammount will be used to buy research items in the next year.
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