2019 Fiscal Year Annual Research Report
The roles of PIGA in epileptic encephalopathy and mental retardation
| Project/Area Number |
17K18362
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| Research Institution | University of Tsukuba |
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Principal Investigator |
張 王其 筑波大学, 人間系, 助教 (20525604)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Keywords | PIGA, / epilepsy, / E/I balance, / hippocampus, / thalamus, / ataxia, / learning and memory, / synapse |
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| Outline of Annual Research Achievements |
By using conditional knockout system, we successfully obtained mice with the ablation of PIGA specifically in inhibitory neurons(IKO), in forebrain excitatory neurons(EKO) and in thalamus neurons(TKO).The behavioral, EEG, histology and electrophysiology analysis showed that IKO and EKO but not TKO mice performed poorly in learning and memory task. IKO and EKO also showed obvious ataxia. It is notable that the susceptibility to kainic acid induced seizures was significantly increased in all three types of mutants. The synaptic density was changed in specific hippocampal subregions of all the mutants. There was a significant E/I balance change in IKO and EKO mice. In addition, AAV mediated PIGA expression in hippocampus could significantly suppress Kainic induced seizure in IKO and EKO.
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Research Products
(17 results)
