Simultaneous visualization of transcriptional activity and 3D genomic structure

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K18363
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology; General anatomy (including histology/embryology)
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Ariyoshi Tetsuro 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (00782103)
• Project Period (FY): 2017-04-01 – 2019-03-31
• Keywords: RNA / 技術開発 / イメージング

Outline of Final Research Achievements

The investigator used Romanesco, a fluorogenic RNA previously developed by the investigator, and succeeded in enhancing the sensitivity in Romanesco-based live-cell RNA imaging. Visualizing endogenous gene transcriptional dynamics at single-cell resolution has been achieved and transcriptional oscillation at single-cell level has been discovered. Simultaneous imaging of gene transcriptional dynamics and genomic DNA structural changes was also successful.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究の成果により遺伝子の発現が一つ一つの細胞の中でどのように変化していくのか計測できる技術基盤が確立された。細胞ごとの遺伝子発現の違いは人体を始めとする複雑な生命構造を形作る基盤となるメカニズムであるので、今後本研究成果の応用により幅広い生命科学分野で様々な発見が生み出されるであろうと期待される。