Induction of Intercalary regeneration in chicken limb buds

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K19400
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Biology of Cells to Organisms, and related fields
• Research Institution: Okayama University
• Principal Investigator: SATOH AKIRA 岡山大学, 異分野融合先端研究コア, 准教授 (90512004)
• Project Period (FY): 2017-06-30 – 2019-03-31
• Keywords: 四肢再生

Outline of Final Research Achievements

Our previous studies clearly shown that Fgf2+Fgf8+Bmp7 are the regeneration inducers in urodele amphibians. The identified regeneration inducers can induce regeneration responses in varied organs and multiple species. In the present study, we tried to apply our defined molecules onto chicken limb buds in order to withdraw potential regeneration ability. Amphibian limb regeneration takes so called a intercalation mechanism. Chicken limb buds do not have the intercalary regeneration mechanism. However, we demonstrated that the ectopic Fgf2+Fgf8 expression in a chicken limb bud resulted in induction of interacalary responses. We also performed cell lineage analysis using a chick-quail chimera analysis. Our results indicate that the conserved regeneration mechanism can induced by Fgf2+Fgf8 inputs even in non-regenerative animals.

Free Research Field

再生生物学

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、両生類型の再生メカニズムが羊膜類で聞こうとしては残存していることを示した初めての成果として意義深いものと考える。その意義に関しては総説として発表し、今後の研究展開の指針とした。また、社会的なメッセージとしては、単に両生類のバイオロジーとして帰結するのではなく、よりヒトに近い動物に応用することを「実働」している研究の姿勢を示すことで、市井の方々に基礎研究の重要性を認識してもらう事ができた。