2019 Fiscal Year Final Research Report
Development of coupled assay-based fluorescent probes to monitor post-translational modification reactions in live cells
| Project/Area Number |
17K19477
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pharmaceutical Sciences and related fields
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Komatsu Toru 東京大学, 大学院薬学系研究科(薬学部), 特任助教 (40599172)
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| Project Period (FY) |
2017-06-30 – 2020-03-31
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| Keywords | ケミカルバイオロジー / 創薬化学 |
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| Outline of Final Research Achievements |
We have developed the methodology of "live" coupled assay that is designed to monitor the activities of enzymes in live cell context. We have successfully developed the systems below to reveal the potential of our methodology in understanding and controlling pathogenic enzymatic functions.
(1) Discovery of compounds to control pathogenic S-adenosylmethionine levels of colorectal tumor cells (JACS 2020).
(2) Development of enzymomics methodologies of monitoring and controlling altered amino acid metabolisms of tumor cells.
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| Free Research Field |
ケミカルバイオロジー
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| Academic Significance and Societal Importance of the Research Achievements |
本研究課題を通じて，生きた細胞内の代謝活性を評価する新たな方法論の確立に成功し，その有用性を世界に発信することができた（JACS 2020）．
また，本研究を通じて見出された (1) 茶カテキンの細胞内メチル化状態の制御活性，(2) 既知の抗がん剤による細胞内代謝活性制御機構の発見，のいずれもが社会的にも重要なインパクトを与える研究成果であり，特に前者については，研究代表者の所属機関らによるプレスリリースがおこなわれ，医療系のニュースサイトなどで取り上げられた．
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