Configuration of cell axes for the development of cell authentication system

2018 Fiscal Year Final Research Report

• Project/Area Number: 17K19507
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical structure and function and related fields
• Research Institution: Hokkaido University
• Principal Investigator: Ohba Yusuke 北海道大学, 医学研究院, 教授 (30333503)
• Project Period (FY): 2017-06-30 – 2019-03-31
• Keywords: 細胞形態 / 細胞内小器官 / 細胞認証

Outline of Final Research Achievements

In order to set a "template" for authenticating cells and a "coordinate axis" for describing it, parameters regarding the arrangement of each organelle were obtained. For this purpose, an organelle marker library was constructed using various fluorescent proteins. It was found that the facilitation of the folding of fluorescent proteins might negatively affect the localization of the organelle markers. In addition, by analyzing the arrangement of organelles using the library, it was found that the centroids of the endosomal system formed a certain line in cells.

Free Research Field

生理学、細胞生物学

Academic Significance and Societal Importance of the Research Achievements

本研究成果により、これまで経験則にのみ基づいていた細胞の形態認識に客観的な指標を与え、生物を題材とした研究ゆえのheterogeneityを説明するような知見が提供されることが期待できる。