Construction of enzymatic polymers for efficient sequential enzymatic reactions in liposomes

2023 Fiscal Year Final Research Report

• Project/Area Number: 17KK0107
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Gunma University (2018-2023); Kanagawa Institute of Industrial Sclence and Technology (2017)
• Principal Investigator: Kamiya Koki 群馬大学, 大学院理工学府, 助教 (70612315)
• Project Period (FY): 2018 – 2023
• Keywords: 酵素 / 逐次反応 / DNAナノテクノロジー / バイオセンサ

Outline of Final Research Achievements

The formation of enzyme-DNA complexes was confirmed by dynamic light scattering and electrophoresis after mixing three distinct enzyme-DNAs. These complexes demonstrated an increased yield of the final product. The subsequent introduction of three miRNAs to the complexes reduced their particle size, as established by dynamic light scattering. Transmission electron microscopy provided evidence of the complexes’ disintegration. Additionally, the presence of the three miRNAs decreased the amount of the final product.

Free Research Field

生体関連化学

Academic Significance and Societal Importance of the Research Achievements

１つの酵素に2本以上の1本鎖DNAを付加させることにより、酵素がネットワーク的に結合することで酵素複合体が形成された。miRNAやRNase等の外部刺激によって、複合体の形成や解離の制御に成功した。これは、酵素による産生物のON/OFFに繋がる。また、この酵素複合体システムは高効率な物質産生のみならず、疾患診断にも応用可能である。