2010 Fiscal Year Final Research Report
Gene Manipulation of Huge DNA by Super Artificial Restriction Enzyme
| Project/Area Number |
18001001
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| Research Category |
Grant-in-Aid for Specially Promoted Research
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
Chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KOMIYAMA Makoto The University of Tokyo, 先端科学技術研究センター, 教授 (50133096)
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| Co-Investigator(Kenkyū-buntansha) |
ASANUMA Hiroyuki 名古屋大学, 大学院・工学研究科, 教授 (20282577)
SUMAOKA Jun 東京大学, 先端科学技術研究センター, 講師 (10280934)
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| Project Period (FY) |
2006 – 2009
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| Keywords | DNA / 制限酵素 / ペプチド核酸 / 遺伝子組換え / セリウム |
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| Research Abstract |
We developed man-made tools (super artificial restriction enzyme) which cut double-stranded DNA at desired site with desired site-specificity. Even huge genomic DNA (e.g., the genome of human beings composed of 3x10^9 base-pairs) was selectively hydrolyzed at one target site. The scission fragments were connected with foreign DNA using DNA ligase, and the recombinant DNA was successfully expressed in mammalian cells. Furthermore, the site-selective scissions by the super artificial restriction enzyme were satisfactorily recognized by the repair system in human cells and promoted the desired homologous recombination. High potential of the super artificial restriction enzyme for future applications has been indicated.
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Research Products
(29 results)
