ショウジョウバエfru遺伝子による脳神経系と行動の性決定機構に関する研究

2009 Fiscal Year Annual Research Report

• Project/Area Number: 18002012
• Research Institution: Tohoku University
• Principal Investigator: 山元 大輔 Tohoku University, 大学院・生命科学研究科, 教授 (50318812)
• Co-Investigator(Kenkyū-buntansha): 小金澤 雅之 東北大学, 大学院・生命科学研究科, 助教 (10302085) ; 木村 賢一 北海道教育大学, 教育学部, 教授 (80214873)
• Keywords: フェロモン受容体 / ChIP-on-chip / クロマチン / Caイメージング / 性転換 / 性モザイク / 求愛行動 / 雄特異的ニューロン

Research Abstract

1.脳と行動の雄化に関わるfru遺伝子ネットワークの解明
パートナータンパク質の同定
fruの機能低下変異体雄においてEcR(エクダイソン受容体)の働きをRNAiにより抑制すると、性的二型を示すmALニューロンの雄特異的な同側神経突起が失われ、部分的に雌化されることが明らかになった。しかし、反対側突起には変化は生じなかった。
一方fruの機能低下変異体雄においてlola(longitudinal lacking)の働きをRNAiにより抑制すると、mALニューロンの反対側突起先端部の形状が、雌様の分枝パターンを呈し、部分的な雌化が認められた。しかし、同側突起には変化がなかった。
このことから同側突起と反対側突起の性差が、独立の機構によって形成されると推察された。
標的遣伝子の同定
Hb遺伝子の転写物をRT-PCRによって検出したところ、fru突然変異体では雄特異的に発現量が低下していることが判明した。表現型レベルでHbがfruと相互作用を示すことを考慮すると、Hbがfruの下流で働く可能性が示唆される。
II.雄の性指向性を支配するニューロン群の同定
fru発現ニューロン全てに高温感受性dTrpAlチャンネルを発現させ、高温シフト刺激を与えたところ、一頭単独で実験容器に入れられた雄が、雌のいない状態で典型的な求愛行動を示した。
そこで、MARCM法を適用し、少数のfru発現ニューロン群にのみdTrpAlを発現させて同様の実験を行ったところ、求愛行動の一部を示す個体が得られた。これらの個体について、行動アッセイ後に脳を取り出し、dTrpAlを発現していたニューロンを同定した。その結果、雄特異的P1介在ニューロンにdTrpAlを発現していた個体が、有意に高い割合で求愛行動を示すことが明らかとなり、P1が雄の性行動のトリガーニューロンとして機能することが立証された。

Research Products

• [Journal Article] Phenotypic interactions of spinster with the genes encoding proteins for cell death control in Drosophila melanogaster (2010)
  • Author(s): Sakurai, A., Nakano, Y., Koganezawa, M., Yamamoto, D.
  • Journal Title: Arch.Insect.Biolchem.Physiol. 73 Pages: 119-127
  • Peer Reviewed
• [Journal Article] The shaping of male courtship posture by lateralized gustatory inputs to male-specific interneurons (2010)
  • Author(s): Koganezawa, M., Haba, D., Matsuo, T., Yamamoto, D.
  • Journal Title: Curr.Biol. 20 Pages: 1-8
  • Peer Reviewed
• [Presentation] 宿主の細胞機能を操作する Wolbachia タンパク質の同定 (2010)
  • Author(s): 大手学, 上山盛夫, 山元大輔
  • Organizer: 第54回日本応用動物昆虫学会大会
  • Place of Presentation: 千葉大学西千葉キャンパス
  • Year and Date: 2010-03-26
• [Presentation] Hunchback cooperates with fruitless to masculinize neurons in the Drosophila brain (2009)
  • Author(s): Goto, J., Mikawa, Y., Ito, H., Yamamoto, D.
  • Organizer: The 32^<nd> Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 20091209-20091212
• [Presentation] Recruitment of chromatin regulators Rpd3 and HP1 to fruitless-containing complex controls neural masculinization in Drosophila (2009)
  • Author(s): Ito, H., Koganezawa, M., Ote, M., Matsumoto, K., Hama, C., Yamamoto, D.
  • Organizer: The 32^<nd> Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 20091209-20091212
• [Presentation] Drosophila fruitless specifies the sex-specific neuronal projection pattern in sensory neurons (2009)
  • Author(s): 木村賢一, 漆崎彬, 松田理恵, 山元大輔
  • Organizer: The 32^<nd> Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Pacifico Yohohama
  • Year and Date: 20091209-20091212
• [Presentation] An attempt at identifying target genes of fruitless in Drosophila (2009)
  • Author(s): Nomoto, S., Toba, G., Nojima, T., Terashima, J., Kurata, S., Yamamoto, D.
  • Organizer: The 32^<nd> Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 20091209-20091212
• [Presentation] Multidendritic sensory neurons in the adult Drosophila abdomen : origins, dendritic remodeling, and life-long maintenance of dendritic arbors (2009)
  • Author(s): 下野耕平, 木村賢一, 藤本梓, 津山泰一, 碓井理夫, 上村匡
  • Organizer: The 32^<nd> Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 20091209-20091212
• [Presentation] Sexual dimorphism in Drosophila gustatory sensory neurons (2009)
  • Author(s): 漆崎彬, 松田理恵, 山元大輔, 木村賢一
  • Organizer: The 32^<nd> Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 20091209-20091212
• [Presentation] The expression of MSin1/Spns1 during the formation of somites (2009)
  • Author(s): Yanagisawa, H., Toyoda, M., Nakano, Y., Kawano, H., Sango, K., Yamamoto, D.
  • Organizer: The 32^<nd> Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 20091209-20091212
• [Presentation] Pheromonal signal shapes courtship song of Drosophila (2009)
  • Author(s): 小金澤雅之, 山元大輔
  • Organizer: The 7^<th> International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
  • Place of Presentation: Fukuoka
  • Year and Date: 20091103-20091104
• [Presentation] ショウジョウバエ運動神経系の性差形成-雄特異筋を支配する運動神経 (2009)
  • Author(s): 木村賢一, 山元大輔
  • Organizer: 第34回日本比較内分泌学会大会・日本比較生理生化学会第31回大会合同大会
  • Place of Presentation: 千里ライフサイエンスセンター, 大阪
  • Year and Date: 20091022-20091024
• [Presentation] MARCM-based analysis of mating behavior of Drosophila (2009)
  • Author(s): Yamamoto, D.
  • Organizer: Janelia Farm Conference : Neural Circuits Controlling Sexual Behavior
  • Place of Presentation: Washington, USA
  • Year and Date: 20091011-14
• [Presentation] Functional dissection of the neural circuitry controlling male courtship by the manipulation of single neuron activities (2009)
  • Author(s): 小金澤雅之, 山元大輔
  • Organizer: Janelia Farm Conference : Improving the Toolkit for Drosophila Neurogenetics
  • Place of Presentation: Washington, USA
  • Year and Date: 20091004-20091007
• [Presentation] Visualizing central neuron activities that can initiate male courtship (2009)
  • Author(s): Kohatsu, S., Yamamoto, D.
  • Organizer: Cold Spring Harbor Laboratory Neurobiology of Drosophila
  • Place of Presentation: New York, USA
  • Year and Date: 20090929-20091003
• [Presentation] Identification of a single motoneuron that induces a male-specific muscle in Drosophila (2009)
  • Author(s): Nojima, T., Kimura, K-I., Matsumoto, K., Nomoto, S., Yamamoto, D.
  • Organizer: Cold Spring Harbor Laboratory Neurobiology of Drosophila
  • Place of Presentation: New York, USA
  • Year and Date: 20090929-20091003
• [Presentation] Spinster gene function is required in a subset of brain neurons for acquiring sexual receptivity in female Drosophila (2009)
  • Author(s): Sakurai, A., Nakano, Y., Koganezawa, M., Yamamoto, D.
  • Organizer: Cold Spring Harbor Laboratory Neurobiology of Drosophila
  • Place of Presentation: New York, USA
  • Year and Date: 20090929-20091003
• [Presentation] ショウジョウバエ前肢味覚感覚ニューロンにおける性差(1)中枢投射パターン (2009)
  • Author(s): 漆崎彬, 松田理恵, 山元大輔, 木村賢一
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡大学
  • Year and Date: 20090917-20090919
• [Presentation] ショウジョウバエ前肢味覚感覚ニューロンにおける性差(2)性決定因子による性差形成 (2009)
  • Author(s): 木村賢一, 漆崎彬, 松田理恵, 山元大輔
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡大学
  • Year and Date: 20090917-20090919
• [Presentation] Sexually dimorphic fruitless-expressing interneurons shape male courtship posture in Drosophila (2009)
  • Author(s): 小金澤雅之, 山元大輔
  • Organizer: The 32^<nd> Annual Meeting the Japan Neuroscience Society
  • Place of Presentation: Nagoya
  • Year and Date: 20090916-20090918
• [Presentation] Identification of the motoneurons that induce a male-specific muscle in Drosophila (2009)
  • Author(s): Nojima, T., Matsumoto, K, Nomoto, S., Yamamoto, D.
  • Organizer: The 32^<nd> Annual Meeting the Japan Neuroscience Society
  • Place of Presentation: Nagoya
  • Year and Date: 20090916-20090918
• [Presentation] フェロモン受容体Gr32a発現ニューロンはショウジョウバエの求愛行動の制御に関与する (2009)
  • Author(s): 小金澤雅之, 山元大輔
  • Organizer: 日本味と匂学会第43回大会
  • Place of Presentation: 旭川
  • Year and Date: 20090902-20090904
• [Presentation] The possible involvement of hunchback and Kruppel in the fruitless-dependent masculinization of neurons (2009)
  • Author(s): Goto, J., Mikawa, Y., Ito, H., Yamamoto, D.
  • Organizer: The 2^<nd> Brain Science Summer Retreat "How to research unsolved problems"(Tohoku Neuroscience GCOE)
  • Place of Presentation: Tohoku University, Sendai
  • Year and Date: 20090725-20090726
• [Presentation] Btk29A-dependent tyrosine phosphorylation of Armadillo/β-catenin is essential for soma-to-germ signaling in Drosophila oogenesis (2009)
  • Author(s): Hamada, N., Yamamoto, D.
  • Organizer: The 2^<nd> Brain Science Summer Retreat "How to research unsolved problems"(Tohoku Neuroscience GCOE)
  • Place of Presentation: Tohoku University, Sendai
  • Year and Date: 20090725-20090726
• [Presentation] Roles of chromatin regulators HP1 and Rpd3 on the formation of sexually dimorphic neuronal circuitry in the Drosophila CNS (2009)
  • Author(s): Ito, H., Koganezawa, M., Ote, M., Matsumoto, K., Tateno, M., Hama, C., Yamamoto, D.
  • Organizer: The 2^<nd> Brain Science Summer Retreat "How to research unsolved problems"(Tohoku Neuroscience GCOE)
  • Place of Presentation: Tohoku University, Sendai
  • Year and Date: 20090725-20090726
• [Presentation] Female sexual receptivity regulated by a subset of brain neurons that require spinster gene function (2009)
  • Author(s): Sakurai, A., Yamamoto, D.
  • Organizer: The 2^<nd> Brain Science Summer Retreat "How to research unsolved problems"(Tohoku Neuroscience GCOE)
  • Place of Presentation: Tohoku University, Sendai
  • Year and Date: 20090725-20090726
• [Presentation] The possible involvement of hunchback and Kruppel in the fruitless-dependent masculinization of neurons (2009)
  • Author(s): Goto, J., Mikawa, Y., Ito, H., Yamamoto, D.
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
  • Year and Date: 20090706-20090708
• [Presentation] Identification of a motoneuron innervating the male specific muscle(Muscle of Lawrence) (2009)
  • Author(s): 木村賢一, 山元大輔
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
  • Year and Date: 20090706-20090708
• [Presentation] Genetic evidence that proteins for RNA metabolism are essential for long-term courtship memory (2009)
  • Author(s): Kimura, S., Sunouchi, K., Nomoto, S., Sakakibara, Y., Yamamoto, D.
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
  • Year and Date: 20090706-20090708
• [Presentation] Regulation of Drosophila oogenesis by Btk29A, a Tec family of tyrosine kinase (2009)
  • Author(s): Kuwada, Y., Hamada, N., Sato, K., Yamamoto, D.
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
  • Year and Date: 20090706-20090708
• [Presentation] A possible role of EcR in the development of fruitless-expressing neurons in Drosophila melanogaster (2009)
  • Author(s): Masuda, T., Ito, H., Yamamoto, D.
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
  • Year and Date: 20090706-20090708
• [Presentation] Identification of the motoneurons required for the formation of the male-specific muscle in Drosophila (2009)
  • Author(s): Nojima, T., Matsumoto, K., Nomoto, S., Yamamoto, D.
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
  • Year and Date: 20090706-20090708
• [Presentation] The analysis of female sexual receptivity with special reference to spinster, a mutation that causes enhanced mate refusal in female Drosophila (2009)
  • Author(s): Sakurai, A., Nakano, Y., Koganezawa, M., Yamamoto, D.
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
  • Year and Date: 20090706-20090708
• [Presentation] Tracing synaptic connections in the Drosophila nervous system by GRASP (2009)
  • Author(s): Sato, S., Toba, G., Yamamoto, D.
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
  • Year and Date: 20090706-20090708
• [Presentation] Mutlidendritic sensory neurons in the adult Drosophila abdomen : origins, dendritic remodeling, and life-long maintenance of dendritic arbors (2009)
  • Author(s): 下野耕平, 木村賢一, 藤本梓, 津山泰一, 碓井理夫, 上村匡
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
  • Year and Date: 20090706-20090708
• [Presentation] A search for the genomic sequence that defines species differences in a fru-dependent male-specific character (2009)
  • Author(s): Takayanagi, S., Lukacsovich, T., Ote, M., Toba, G., Yamamoto, D.
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
  • Year and Date: 20090706-20090708
• [Presentation] A putative gustatory receptor required for efficient courtship in Drosophila melanogaster (2009)
  • Author(s): Watanabe, K., Toba, G., Yamamoto, D.
  • Organizer: The 9^<th> Japanese Drosophila Research Conference
  • Place of Presentation: Kakegawa
  • Year and Date: 20090706-20090708
• [Presentation] 行動の性差を生み出す脳と遺伝子の機構 (2009)
  • Author(s): 山元大輔
  • Organizer: 第49回生命科学 夏の学校 in 神戸
  • Place of Presentation: 神戸セミナーハウス
  • Year and Date: 2009-08-28
• [Presentation] 接触フェロモン受容によるショウジョウバエ求愛行動の制御 (2009)
  • Author(s): 小金澤雅之, 山元大輔
  • Organizer: 平成21年度日本動物学会東北支部会
  • Place of Presentation: 宮城教育大学, 仙台
  • Year and Date: 2009-08-01
• [Presentation] 性行動の引き金を引く脳細胞を同定する (2009)
  • Author(s): 山元大輔
  • Organizer: 第2回東北大学バイオフォーラム～脳科学の革新的な発展を目指して～
  • Place of Presentation: 東京ステーションコンファレンス(サピアタワービルディング)
  • Year and Date: 2009-06-05
• [Book] 面白いほどよくわかる脳と心 (2010)
  • Author(s): 山元大輔(監修)
  • Total Pages: 222
  • Publisher: 日本文芸社
• [Book] 脳を刺激する習慣 (2009)
  • Author(s): 山元大輔
  • Total Pages: 182
  • Publisher: PHP研究所
• [Book] 最新応用昆虫学 (2009)
  • Author(s): 嶋田透, 嶋田正和, 竹田敏, 鎮西康雄, 寺山守, 山元大輔
  • Total Pages: 264
  • Publisher: 朝倉書店