2010 Fiscal Year Final Research Report
The Mechanism of Intracellular Transport and Kinesin Motors, KIFs : Structure, Function, Dynamics and Regulation
| Project/Area Number |
18002013
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| Research Category |
Grant-in-Aid for Specially Promoted Research
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HIROKAWA Nobutaka The University of Tokyo, 大学院・医学系研究科, 特任教授 (20010085)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKATA Takao 東京大学, 大学院・医学系研究科, 准教授 (50218004)
NODA Yasuko 東京大学, 大学院・医学系研究科, 講師 (50262019)
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| Research Collaborator |
KANAI Yoshimitsu 東京大学, 大学院・医学系研究科, 准教授 (80214427)
TAKEI Yosuke 東京大学, 大学院・医学系研究科, 准教授 (20272487)
OKADA Yasushi 東京大学, 大学院・医学系研究科, 助手 (50272430)
TANAKA Yosuke 東京大学, 大学院・医学系研究科, 助手 (90302661)
NITTA Ryo 東京大学, 大学院・医学系研究科, 助教 (40345038)
HOMMA Noriko 東京大学, 大学院・医学系研究科, 助教 (50345039)
MIKI Harukata 東京大学, 大学院・医学系研究科, 助教 (10361461)
OGAWA Tadayuki 東京大学, 大学院・医学系研究科, 特任助教 (40436572)
YAJIMA Hiroaki 東京大学, 大学院・医学系研究科, 特任研究員 (30376412)
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| Project Period (FY) |
2006 – 2010
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| Keywords | 分子モーター / キネシン / 細胞内輸送 / 微小管 |
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| Research Abstract |
Intracellular transport is fundamental for cellular functions in cells in general. We studied this mechanism focusing on the kinesin superfamily proteins (KIFs). The primary structures of 13 new KIFs were solved. Using molecular cell biology and molecular genetics we revealed that KIF4 is a key molecule determining activity dependent survival or death of juvenile neurons, that KIF26A is fundamental for development of enteric nervous system by acting as a suppressor for GDNF/Ret signaling, and that KIF17 plays a significant role by not only transporting NDMA receptors, but also controlling transcription and translation of NR2B and KIF17 through CREB phosphorylation and ubiquitin-proteasome dependent degradation of NR2A. We solved atomic structures during Mg^<++> and water release from Mg^<++>ADP so that we solved almost all states during ATP hydrolysis which gives us strong bases to understand how KIFs move along microtubules.
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Research Products
(26 results)
