Basic study to establish "humanized animal model" by improvement and modification of severely immunodeficient NOG mouse

2008 Fiscal Year Self-evaluation Report

• Project/Area Number: 18100005
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Central Institute for Experimental Animals
• Principal Investigator: ITO Mamoru Central Institute for Experimental Animals, 免疫研究室, 室長 (00176364)
• Project Period (FY): 2006 – 2009
• Keywords: 実験動物 / 免疫不全マウス / NOGマウス / ヒト化モデル / 発生・分化

Research Abstract

我々が開発した高度免疫不全NOD/SCID/γC^(null)(NOG)マウスは、ヒトの細胞、組織や臓器を高生着させるマウスである。このマウスに様々なヒト因子やサイトカイン等を分泌させることなど、さらに改良を加えることによって、「ヒト化動物モデル」の作出のための基盤となる高度免疫不全マウスを作製する。

Research Products

• [Journal Article] Highly sensitive model for xenogenic GVHD using severe immunodeficient NOG mice (2009)
  • Author(s): Ito, R., I. Katano, K. Kawai, H. Hirata, T. Ogura, T. Kamisako, T. Eto, and M. Ito.
  • Journal Title: Transplantation 87 Pages: 1654-1658
  • Peer Reviewed
• [Journal Article] NOD/Shi-scid IL2rgamma (null) (NOG) micemore appropriate for humanized mouse models (2008)
  • Author(s): Ito, M., K. Kobayashi, and T. Nakahata.
  • Journal Title: Curr Top Microbiol Immunol 324 Pages: 53-76
  • Peer Reviewed
• [Journal Article] Establishment of a humanized model of liver using NOD/Shi-scid IL2Rgnull mice (2008)
  • Author(s): Suemizu, H., M. Hasegawa, K. Kawai, K. Taniguchi, M. Monnai, M. Wakui, M. Suematsu, M. Ito, G. Peltz, and M. Nakamura.
  • Journal Title: Biochem Biophys Res Commun 377 Pages: 248-252
  • Peer Reviewed
• [Journal Article] A new humanized mouse model of Epstein-Barrvirus infection that reproduces persistent infection, lymphoproliferative disorder, and cell-mediated and humoral immune responses (2008)
  • Author(s): Yajima, M., K. Imadome, A. Nakagawa, S. Watanabe, K. Terashima, H. Nakamura, M. Ito, N. Shimizu, M. Honda, N. Yamamoto, and S. Fujiwara.
  • Journal Title: J Infect Dis 198 Pages: 673-682
  • Peer Reviewed
• [Journal Article] Quiescent human hematopoietic stem cells in the bone marrow niches organize the hierarchical structure of hematopoiesis (2008)
  • Author(s): T., Y. Muguruma, S. Yumino, Y. Sheng, T. Uno, H. Matsuzawa, M. Ito, S. Kato, T. Hotta, and K. Ando.
  • Journal Title: Stem Cells 26 Pages: 3228-3236
  • Peer Reviewed
• [Presentation] 「ヒト幹細胞分化と維持モデル」シンポジウム「ヒト化動物の現状と今後」 (2009)
  • Author(s): 安藤潔、八幡崇、六車ゆかり
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮
  • Year and Date: 20090514-16
• [Presentation] 「ヒト血液幹細胞移植マウスのウイルス病原性解析への利用」シンポジウム「ヒト化動物の現状と今後」 (2009)
  • Author(s): 小柳義夫、佐藤佳、伊藤守
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮
  • Year and Date: 20090514-16
• [Presentation] Mouse models as "humanized mice" (2009)
  • Author(s): Ito M.
  • Organizer: 2^<nd> International workshop of humanized mice
  • Place of Presentation: Holland
  • Year and Date: 20090403-06
• [Presentation] C-kit変異をもつ重度免疫不全NOG-Wvマウスはヒト細胞の高い生着性を示す (2008)
  • Author(s): 片野いくみ
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 20081201-20081203
• [Presentation] Dendritic cells have a suppressive effect on xeno-GVHD in novel immunodeficient NOG mice (2008)
  • Author(s): 伊藤亮治
  • Organizer: 第10回国際樹状細胞シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 20081001-20081005
• [Remarks] 1. 朝日新聞記事「平成20年9月15日」
• [Remarks] 2. 朝日新聞「平成18年10月20日」
• [Remarks] 3. 日経産業新聞「平成18年10月11日」
• [Remarks]
  • URL: http://www.ciea.or.jp
• [Patent(Industrial Property Rights)] Methods of constructing mouse suitable for the take, differentiation and proliferation of heterogenous cells, mouse constructed by the method and use of the mouse (2007)
  • Inventor(s): M, Ito, et.al.
  • Industrial Property Rights Holder: Central Institute for Experimental Animals
  • Industrial Property Number: EP 1,338,198 B1
  • Acquisition Date: 2007-10-28
  • Overseas
• [Patent(Industrial Property Rights)] Methods of producing a mouse suitable for the engraftment, differentiation and proliferation of heterologous cells, mouse produced by this method and use of the mouse (2006)
  • Inventor(s): M, Ito, et.al.
  • Industrial Property Rights Holder: Central Institute for Experimental Animals
  • Industrial Property Number: US 7,145,055 B2
  • Acquisition Date: 2006-12-05
  • Overseas
• [Patent(Industrial Property Rights)] 異種細胞の生着、分化および増殖に適したマウスの作出方法、該方法により作出されたマウスならびにそのマウスの用途 (2005)
  • Inventor(s): 伊藤守, 他9名
  • Industrial Property Rights Holder: 財団法人実験動物中央研究所
  • Industrial Property Number: 日本特許第3753321号
  • Acquisition Date: 2005-11-16