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2009 Fiscal Year Final Research Report

Multifunctional Nano-Scanning Electrochemical Microscopy

Research Project

  • PDF
Project/Area Number 18101006
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Microdevices/Nanodevices
Research InstitutionTohoku University

Principal Investigator

MATSUE Tomokazu  Tohoku University, 大学院・環境科学研究科, 教授 (70173797)

Co-Investigator(Kenkyū-buntansha) MIZUTANI Fumio  兵庫県立大学, 大学院・物質理学研究科, 教授 (80118603)
YANAGISAWA Teruyuki  東北大学, 大学院・医学系研究科, 教授 (90133941)
SHIKU Hitoshi  東北大学, 大学院・環境科学研究科, 准教授 (10361164)
YASUKAWA Tomoyuki  兵庫県立大学, 大学院・物質理学研究科, 准教授 (40361167)
YAMADA Hiroshi  防衛大学校, 応用化学科, 准教授 (10545974)
Co-Investigator(Renkei-kenkyūsha) AKIBA Uichi  秋田大学, 工学資源学部, 准教授 (60184107)
Project Period (FY) 2006 – 2009
Keywordsマイクロ化学システム / 単一細胞計測
Research Abstract

The multifunctional micro/nano scanning electrochemical imaging system (NanoSECM) was developed and utilized for the single-cell diagnostics. We fabricated multifunctional micro/nano-structured probe electrodes and developed a novel feedback control between the electrode and sample surfaces at nanometer levels. By using NanoSECM with these new technologies, we successfully attained the fast and high resolution and simultaneous imaging of live cells to disclose the cell functions.

  • Research Products

    (30 results)

All 2010 2009 2008 2007 2006 2005 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (13 results) Book (4 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Simultaneous Noncontact Topography and Electrochemical Imaging by SEC M/SICM Featuring Ion Current Feedba ck Regulation.2010

    • Author(s)
      Y.Takahashi, A.I.Shev chuk, P.Novak, Y.Murakami, H.Shiku, Y.E.Korchev, T.Matsue
    • Journal Title

      J.Am.Chem.Soc. 132

      Pages: 10118-10126

    • Peer Reviewed
  • [Journal Article] Y. Takahashi, Y. Murakami, K. Nagamine, H. Shiku, S. Aoyagi, T. Yasukawa, M. Kanzaki, T. Matsue2010

    • Author(s)
      Topographic Imaging of Convoluted Live Cells by Scanning Ion Conductance Micr oscopy in a Standing Approach Mode
    • Journal Title

      Phys.Chem.Chem.Phys. 12

      Pages: 10012-17

    • Peer Reviewed
  • [Journal Article] Transfected Single-Cell Imaging by Scan ping Electrochemical Optical Microscopy with Shear Force Feedback Regulation.2009

    • Author(s)
      Y.Takahashi, H.Shiku, T.Murata, T.Yasukawa, T.Matsue
    • Journal Title

      Anal.Chem. 81

      Pages: 9674-9681

    • Peer Reviewed
  • [Journal Article] Electrochemical Single-Cell Gene Expression Assay Combining Dielectrophoretic M anipulation with Secreted Alkaline Phospha tase Reporter System.2009

    • Author(s)
      T.Murata, T.Yasukawa, H.Shiku, T.Matsue
    • Journal Title

      Biosens. Bioelectron. 25

      Pages: 913-919

    • Peer Reviewed
  • [Journal Article] Electrochemical Detection of Epidermal Growth Factor Receptors on a Single Living Cell Surface by Scanning Electrochemical Microscopy.2009

    • Author(s)
      Y.Takahashi, T.Miyamoto, H.Shiku, R.Asano, T.Yasukawa, I.Kumagai, T.Matsue
    • Journal Title

      Anal.Chem. 81

      Pages: 2785-2790

    • Peer Reviewed
  • [Journal Article] Takemoto J, Masumiya H, Nunoki K, Sato T, Nakagawa H, Ikeda Y, Arai, Yanagisawa Y2008

    • Author(s)
      Potentiation of potassium currents by beta-adrenoceptor agonists in human urinary bladder smooth muscle cells : a possible electrical mechanism of relaxation.
    • Journal Title

      Pharmacology 81

      Pages: 251-258

    • Peer Reviewed
  • [Journal Article] Fabrication of a shear force-based ion-se lective capillary probe for scanning electro chemical microscopy.2008

    • Author(s)
      H.Yamada, Y.Ikut a Y, T.Koike T, T.Matsue
    • Journal Title

      Chem.Lett. 37

      Pages: 392-393

    • Peer Reviewed
  • [Journal Article] Measurement of Gene Expression from Single Adherent Cells and Spheroids Colle cted Using Fast Electrical Lysis.2007

    • Author(s)
      Y Nashimoto, Y.Takahashi, T.Yamakawa, Y.Torisawa, T.Yasukawa, T.Ito-Sasaki, Mi Yokoo, H.Abe, H.Shiku, H.Kambara, T.Matsue
    • Journal Title

      Anal.Chem. 79

      Pages: 6823-6830

    • Peer Reviewed
  • [Journal Article] Microcontact printed diaphorase monolay er on glass characterized by atomic force microscopy and scanning electrochemical microscopy2007

    • Author(s)
      H.Q.Luo, H.Shiku, A.Kumagai, Y.Takahashi, T.Yasukawa, T.Matsue
    • Journal Title

      Electrochemistry Communications 9

      Pages: 2703-2708

    • Peer Reviewed
  • [Journal Article] Electrochemical screening of recombinant protein solubility in Escherichia coli using scanning electrochemical microscopy (SECM)2007

    • Author(s)
      K.Nagamine, S.Onodera, A.Kurihara, T.Yasukawa, H.Shiku, R.Asano, I.Kumagai, T.Matsue
    • Journal Title

      Biotechnology and bioengineering 96

      Pages: 1008-1013

    • Peer Reviewed
  • [Journal Article] Topographic, Electrochemical, and O ptical Images Captured Using Standing Approach Mode Scanning Electrochemic al/Optical Microscopy.2006

    • Author(s)
      Y.Takahashi, Y.Hirano, T.Yasukawa, H.Shiku, H.Yamada, T.Matsue
    • Journal Title

      Langmuir 22

      Pages: 10299-10306

    • Peer Reviewed
  • [Presentation] Integrated micro/nano electrochemical systems for bioanalysis2009

    • Author(s)
      T.Matsue
    • Organizer
      NIMS WEEK 2009
    • Place of Presentation
      エポカルつくば
    • Year and Date
      2009-07-22
  • [Presentation] Cav channels and their modifiers : molecular identification of the binding sites.2009

    • Author(s)
      T.Yana gisawa
    • Organizer
      10th International Symposium on Mechaisms of Vasodilatation
    • Place of Presentation
      松島
    • Year and Date
      2009-06-01
  • [Presentation] Electrochemical Detection of Gene Expression in a Single Living Cell2008

    • Author(s)
      T.Matsue
    • Organizer
      CRC Symposium
    • Place of Presentation
      札幌
    • Year and Date
      2008-10-29
  • [Presentation] Characterization of Cellular Functions with Scanning Electrochemical Microcopy2008

    • Author(s)
      T.Matsue
    • Organizer
      Electrochemistry and Biomedical technology Symposium
    • Place of Presentation
      ソウル(韓国)
    • Year and Date
      2008-10-06
  • [Presentation] Single-cell analysis of nucleic acids and proteins in gene introduction system2008

    • Author(s)
      H.Shiku, D.Okazaki, T.Yamakawa, H.Akita, H.Harashima, T.Matsue
    • Organizer
      The 3 rd International Workshop on approaches to Single-Cell Analysis
    • Place of Presentation
      ETH Zurich, Switzerland
    • Year and Date
      2008-09-11
  • [Presentation] Electrochemical Analysis of Yeast Cells Expressing Beta-Galactosidase within a Poly (dimethylsiloxane) Microwell Array2008

    • Author(s)
      H.Shiku, S.Goto, K.Nagamine, T.Yasukawa, T.Itayama, T.Matsue
    • Organizer
      The 5th Workshop on Scanning Electrochemical Microscopy
    • Place of Presentation
      Blue Mountain Lake (米国)
    • Year and Date
      2008-08-24
  • [Presentation] Integrated Electrochemical Devices for Detection of Gene Expression2008

    • Author(s)
      H.Shiku
    • Organizer
      3rd German-Italian-Japanese Meeting of Electrochemists
    • Place of Presentation
      Taormina, Itaria
    • Year and Date
      2008-05-25
  • [Presentation] Integrated Electrochemical Cellular Systems for Gene Engineering.2008

    • Author(s)
      T.Matsue
    • Organizer
      6th Asian Conference on Electrochemistry in Taipei
    • Place of Presentation
      Taipei
    • Year and Date
      2008-05-12
  • [Presentation] 微小イオン選択性電極を探針としたシアフォース制御走査型電気化学顕微鏡による形状・局所カリウムイオン濃度の同時測定2008

    • Author(s)
      山田弘,宮澤春菜,小池亨
    • Organizer
      電気化学会第75回大会
    • Place of Presentation
      山梨大学
    • Year and Date
      2008-03-31
  • [Presentation] Electrochemical Monitoring of Gene Expression with Cellular Devices2007

    • Author(s)
      T.Matsue
    • Organizer
      The 9th Asian Conferences on Analytical Sciences
    • Place of Presentation
      Jeju, Korea(基調講演)
    • Year and Date
      2007-11-06
  • [Presentation] Dual-Capillary Micro-fluidic Probe to collect mRNA from a single abherent cells2007

    • Author(s)
      H.Shiku, T.Yamakawa, Y.Nashimoto, Y.Takagasgu, Y.Torisawa, T.Yasukawa, T.Ito-Sasaki, M.Yokoo, H.Abe, H.Kambara, T.Matsue
    • Organizer
      The International Forum on Post-Genome Technologies
    • Place of Presentation
      中国
    • Year and Date
      2007-09-09
  • [Presentation] 集積型細胞デバイスを用いた遺伝子発現の検出2007

    • Author(s)
      末永智一
    • Organizer
      第15回化学とマイクロシステム研究会
    • Place of Presentation
      仙台
    • Year and Date
      2007-05-25
  • [Presentation] マイクログルコースセンサをプローブとした電気化学顕微鏡の開発2005

    • Author(s)
      安川智之,水谷文雄
    • Organizer
      表面技術協会第ll7回講演大会
    • Place of Presentation
      千葉
    • Year and Date
      2005-03-12
  • [Book] 化学フロンティア「切り拓き,サイエンスを牽引する分析最前線(微生物機能を利用したマイクロバイオチップの開発)2008

    • Author(s)
      長峯邦明,末永智一
    • Total Pages
      127-133
    • Publisher
      化学同人
  • [Book] 先進化学センサ(細胞機能を利用するマイクロバイオセンサ)2008

    • Author(s)
      井上(安田)久美,珠玖仁,末永智一
    • Total Pages
      243-252
    • Publisher
      (株)ティー・アイ・シィー
  • [Book] Handbook of Bioelectrochemistry(Whole-Cell Biosensors)2008

    • Author(s)
      H. Shiku. K. Nagamine, T. Kaya, T. Yasukawa, T. Matsue
    • Total Pages
      Chapter 7(249-266)
    • Publisher
      John Wiley
  • [Book] バイオセンサ・ケミカルセンサ事典(マイクロフルーイディックシステムを用いた細胞デバイス)2007

    • Author(s)
      鳥澤勇介,安川智之,珠玖仁,末永智一
    • Publisher
      テクノシステム
  • [Remarks] ホームページ等

    • URL

      http://www.che.tohoku.ac.jp/~bioinfo/

  • [Patent(Industrial Property Rights)] 走査型電気化学イオンコンダクタンス顕微鏡測定法,走査型電気化学イオンコンダクタンス顕微鏡,その探針および探針の製造方法2009

    • Inventor(s)
      高橋康史,末永智一,珠玖仁,村上有美,長峯邦明,アンドリュー・シェヴチャク,ユーリ・コルチェフ
    • Industrial Property Rights Holder
      国立大学法人東北大学,インペリアル・イノベーションズ・リミティッド
    • Industrial Property Number
      特願2009-140602
    • Filing Date
      2009-06-11

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Published: 2012-01-26   Modified: 2016-04-21  

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