2010 Fiscal Year Final Research Report
Studies on vertebrate development by the transposon-mediated Gal4 enhancer trap method
| Project/Area Number |
18101008
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
基礎ゲノム科学
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
KAWAKAMI Koichi National Institute of Genetics, 個体遺伝研究系, 教授 (70195048)
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| Project Period (FY) |
2006 – 2010
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| Keywords | ゲノム機能 / 発生遺伝学 |
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| Research Abstract |
We successfully developed the Gal4 enhancer trap and gene trap methods with the Tol2 transposable element, and generated more than 1,000 transgenic fish lines that express Gal4 in spatially and temporally restricted fashions during embryonic development. By using these transgenic fish, we disclosed mechanisms of organogenesis including the lateral line, lymphatic system, lens, retina and notochord. Further, we analyzed behavioral abnormalities caused by inhibition of spinal sensory- and inter-neurons and a specific subset of the olfactory sensory neurons. Furthermore, we visualized firing of motor circuits by calcium imaging. Thus, we established the basis for studies of developmental biology and neuroscience in a vertebrate.
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[Journal Article] The habenula is crucial for experience-dependent modification of fear responses in zebrafish.2010
Author(s)
Agetsuma M., Aizawa H., Aoki T., Nakayama R., Takahoko M., Goto M., Sassa T., Amo R., Shiraki T., Kawakami K., Hosoya T., Higashijima, S., Okamoto H.
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Journal Title
Nature Neuroscience 13
Pages: 1354-1356
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[Journal Article] zebrafish gene trap and enhancer trap database.2010
Author(s)
Kawakami K., Abe G., Asada T., Asakawa K., Fukuda R., Ito A., Lal P., Mouri N., Muto A., Suster M.L., Takakubo H., Urasaki A., Wada H., Yoshida M. zTrap
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Journal Title
BMC Developmental Biology 10
Pages: 105
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[Journal Article] Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions.2010
Author(s)
Yamamoto M., Morita R., Mizoguchi T., Matsuo H., Isoda M., Ishitani T., Chitnis A.B., Matsumoto K., Crump J.G., Hozumi K., Yonemura S., Kawakami K., Itoh M.
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Journal Title
Development 137
Pages: 2527-2537
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[Journal Article] Insertional mutagenesis by the Tol2 transposon-mediated enhancer trap approach generated mutations in two developmental genes : tcf7 and synembryn-like.2008
Author(s)
Nagayoshi S., Hayashi E., Abe G., Osato N., Asakawa K., Urasaki A., Horikawa K., Ikeo, K., Takeda H., Kawakami K.
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Journal Title
Development 135
Pages: 159-169
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[Journal Article] A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart.2007
Author(s)
Seguchi O., Takashima S., Yamazaki S., Asakura M., Asano Y., Shintani Y., Wakeno M., Minamino T., Kondo H., Furukawa H., Nakamaru K., Naito, A., Takahashi T., Ohtsuka T., Kawakami K., Isomura T., Kitamura S., Tomoike H., Mochizuki N., Kitakaze M.
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Journal Title
The Journal of Clinical Investigation 117
Pages: 2812-2824
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