2010 Fiscal Year Final Research Report
Studies of Bioorganicchemistry on Microbial and Plant Peptide Factor
| Project/Area Number |
18101009
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Nagoya University |
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Principal Investigator |
SAKAGAMI Youji 名古屋大学, 生命農学研究科, 教授 (80107408)
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| Co-Investigator(Kenkyū-buntansha) |
OJIKA Makoto 名古屋大学, 大学院・生命農学研究科, 教授 (50152492)
KONDO Tatsuhiko 名古屋大学, 大学院・生命農学研究科, 助教 (30362289)
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| Project Period (FY) |
2006 – 2010
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| Keywords | ペプチド / 翻訳後修飾 / 枯草菌 / フェロモン / クオラムセンシング / イソプレノイド / CLV3 / 気孔 |
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| Research Abstract |
1. We performed the structure elucidation and structure-activity relationship studies on the ComX pheromone which plays an essential role in quorum-sensing of Bacillus subtilis. We also established an in vitro system to detect the characteristic posttranslational modification on the ComX precursor to investigate the mechanisms and the universality of the modification.
2. We optimized the purification techniques to identify novel plant peptide hormones derived from putative precursor genes. Using the methods, we identified a posttranslationally modified peptide, MCLV3, which regulates the function of shoot apical meristem of Arabidopsis thaliana. In addition, we elucidated the structure of the mature form of stomagen, which is a mesophyll-derived factor controlling the stomatal density on the epidermis of Arabidopsis.
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[Journal Article] Geranyl modification on the tryptophan residue of ComX_<RO-E-2> pheromone by a cell-free system2011
Author(s)
Fumitada ; Ishihara, Ayako ; Kurata, Keiko ; Nakagawa, Aya ; Okada, Masahiro ; Kitamura, Shigeyuki ; Kanamaru, Kyoko ; Masuda, Yuichi ; Murakami, Kazuma ; Irie, Kazuhiro ; Sakagami, Youji
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Journal Title
FEBS Letters
Volume: 586(2)
Pages: 174-179
Peer Reviewed
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[Journal Article] Stomatal density is controlled by a mesophyll-derived signaling molecule2010
Author(s)
Kondo, Tatsuhiko ; Kajita, Ryoko ; Miyazaki, Aya ; Hokoyama, Mayumi ; Nakamura-Miura, Touko ; Mizuno, Satoko ; Masuda, Yuichi ; Irie, Kazuhiro ; Tanaka, Yuki ; Takada, Shinobu ; Kakimoto, Tatsuo ; Sakagami, Youji
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Journal Title
Plant and Cell Physiology
Volume: 51(1)
Pages: 1-8
Peer Reviewed
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[Presentation] Stomatal density is controlled by a mesophyll-derived signaling molecule2012
Author(s)
Kondo, T., Kajita, R., Miyazaki, A., Hokoyama, M., Nakamura-Miura, T., Mizuno S., Masuda, Y., Irie, K., Tanaka, Y., Takada, S., Kakimoto, T., Sakagami, Y.
Organizer
第53回日本植物生理学会年会PCP
Place of Presentation
京都
Year and Date
20120316-18
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[Presentation] Geranyl modification on the tryptophan residue of ComX_<RO-E-2> pheromone by cell free system2010
Author(s)
Tsuji, F., Ishihara, A., Nakagawa, A., Masuda, Y., Sugimoto, T., Irie, T., Sakagami, Y
Organizer
The International Chemical Congress of Pacific Basin Societies(Pacifichem)
Place of Presentation
Honolulu, USA
Year and Date
20101115-20
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[Presentation] Stomatal density is controlled by a mesophyll-derived signaling molecule2010
Author(s)
Sakagami, Y., Kondo, T., Kajita, R., Miyazaki, A., Hokoyama, M., Nakamura-Miura, T., Mizuno S., Masuda, Y., Irie, K., Tanaka, Y., Takada, S., Kakimoto, T.
Organizer
The Federation of European Societies of Plant Biology(FESPB)
Place of Presentation
Valencia, Spain
Year and Date
20100705-09
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[Presentation] Stomatal density is controlled by a mesophyll-derived signaling molecule2010
Author(s)
Kondo, T., Kajita, R., Miyazaki, A., Hokoyama, M., Nakamura-Miura, T., Mizuno S., Masuda, Y., Irie, K., Tanaka, Y., Takada, S., Kakimoto, T., Sakagami, Y
Organizer
20th International Conference on Plant Growth Substances
Place of Presentation
Tarragona, Spain
Year and Date
20100628-0701
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