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2010 Fiscal Year Final Research Report

Modification of plant gene expression by viral gene silencing suppressors

Research Project

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Project/Area Number 18108001
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Plant pathology
Research InstitutionHokkaido University

Principal Investigator

UYEDA Ichiro  Hokkaido University, 大学院・農学研究院, 教授 (10113523)

Co-Investigator(Kenkyū-buntansha) MASUTA Chikara  北海道大学, 大学院・農学研究院, 教授 (60281854)
NAKAHARA Kenji  北海道大学, 大学院・農学研究院, 助教 (90315606)
Project Period (FY) 2006 – 2010
Keywords病原体相互作用 / 病原性因子 / サイレンシング / ウイルス
Research Abstract

Plants defense against virus infection by Post Transcriptional Gene Silencing (PTGS). We analyzed how PTGS was involved in pathogenicity and obtained the following results. (1) Suppressor activity of viral HC-Pro gene is required for induction of necrotic symptoms. (2) rgs-CaM gene has affinity to many viral suppressor genes. Its interaction destabilizes bound proteins and consequently strengthen defense against virus infection by elevating PTGS activity. (3) Y satellite RNA of cucumber mosaic virus (Y-satRNA) induces bright yellow mosaic in tobacco instead of regular green mosaic. Molecular mechanism of how Y-satRNA induces yellow mosaic is elucidated.

  • Research Products

    (44 results)

All 2011 2010 2009 2008 2007 2006 Other

All Journal Article (20 results) (of which Peer Reviewed: 18 results) Presentation (23 results) Remarks (1 results)

  • [Journal Article] A viral satellite RNA induces yellow symptoms on tobacco by targeting a gene involved in chlorophyll biosynthesis using the RNA silencing machinery.2011

    • Author(s)
      Shimura H., Pantaleo V., Ishihara T., Myojo N., Inaba J., Sueda K., Burgyan J., Masuta C.
    • Journal Title

      PLoS Pathogens (in press)

    • Peer Reviewed
  • [Journal Article] Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector : Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A.majus.2011

    • Author(s)
      Kim B.M., Inaba J., Masuta C.
    • Journal Title

      Horticulture, Environment, and Biotechnology 52(in press)

    • Peer Reviewed
  • [Journal Article] HC-Pro, a potyvirus RNA silencing suppressor, cancels cycling of Cucumber mosaic virus in Nicotiana benthamiana plants.2010

    • Author(s)
      Fukuzawa N., Itchoda N., Ishihara T., Goto K., Masuta C., Matsumura T.
    • Journal Title

      Virus Genes 40

      Pages: 440-446

    • Peer Reviewed
  • [Journal Article] The C-terminal residues of the 2b protein of Cucumber mosaic virus are important for efficient expression in Escherichia coli and DNA binding.2010

    • Author(s)
      Sueda K., Shimura H., Meguro A., Uchida T., Inaba J., Masuta C.
    • Journal Title

      FEBS Letters 584

      Pages: 945-950

    • Peer Reviewed
  • [Journal Article] A progeny virus from a cucumovirus pseudorecombinant evolved to gain the ability to accumulate its RNA silencing suppressor leading to systemic infection in tobacco.2010

    • Author(s)
      Asaoka R., Shimura H., Arai M., Masuta C.
    • Journal Title

      Molecular Plant-Microbe Interactions 23

      Pages: 32-339

    • Peer Reviewed
  • [Journal Article] The P3 Protein of turnip mosaic virus can alone induce HR-like cell death in Arabidopsis thaliana carrying TuNI.2010

    • Author(s)
      Kim B., Suehiro N., Natsuaki T., Inukai T., Masuta C.
    • Journal Title

      Molecular Plant-Microbe Interactions 40

      Pages: 144-152

    • Peer Reviewed
  • [Journal Article] Involvement of the P1 cistron in overcoming eIF4E-mediated recessive resistance against Clover yellow vein virus in pea.2010

    • Author(s)
      Nakahara K.S., Shimada R., Choi H.S., Yamamoto H., Jun S., Uyeda I.
    • Journal Title

      Moleular Plant Microbe Interactions 23

      Pages: 1460-1469

    • Peer Reviewed
  • [Journal Article] Silencing by small RNAs is linked to endosomal trafficking2009

    • Author(s)
      Lee Y.S., Pressman S., Andress A.P., Kim K., White J.L., Cassidy J.J., Li1 X., Lubel1 K., Lim D.H., Cho I.S., Nakahara K., Preall1 J. B., Bellarel P., Sontheimer1 E.J., Carthew R.W.
    • Journal Title

      Nature Cell Biology 11

      Pages: 1150-1156

    • Peer Reviewed
  • [Journal Article] Eukaryotic initiation vactor 4E controls cyv 2 resistance in pea.2009

    • Author(s)
      Andrade M., Abe Y., Nakahara K. S., Uyeda I.
    • Journal Title

      J Gen Plant Pathol. 75

      Pages: 241-249

  • [Journal Article] Activation of the salicylic acid signaling pathway enhances Clover yellow vein virus virulence in susceptible pea cultivars.2009

    • Author(s)
      Atsumi G., Kagaya U., Kitazawa H., Nakahara K.S., Uyeda I.
    • Journal Title

      Moleular Plant Microbe Interactions 22

      Pages: 166-175

  • [Journal Article] A strategy for screening an inhibitor of viral silencing suppressors, which attenuates symptom development of plant viruses.2008

    • Author(s)
      Shimura H., Fukagawa T., Meguro A., Yamada H., Oh-hira M., Sano S., Masuta C.
    • Journal Title

      FEBS Letters 582

      Pages: 4047-4052

    • Peer Reviewed
  • [Journal Article] Down-regulation of flavonoid 3'-hydroxylase gene expression by virus-induced gene silencing in soybean reveals the presence of a threshold mRNA level associated with pigmentation in pubescence.2008

    • Author(s)
      Nagamatsu A., Masuta C., Matsuura H., Kitamura K., Abe J., Kanazawa A.
    • Journal Title

      Journal of Plant Physiology 5

      Pages: 778-790

    • Peer Reviewed
  • [Journal Article] Degree of RNA silencing and ability of a viral suppressor vary depending on the cell species in a protoplast system.2008

    • Author(s)
      Shimura H., Kogure Y., Goto K., Masuta C.
    • Journal Title

      Journal of General Plant Pathology 74

      Pages: 326-330

    • Peer Reviewed
  • [Journal Article] Point mutations in helper component protease of clover yellow vein virus are associated with the attenuation of RNA-silencing suppression activity and symptom expression in broad bean.2008

    • Author(s)
      Yambao M.L.M., Yagihashi H., Sekiguchi H., Sekiguchi T., Sasaki T., Sato M., Atsumi G., Tacahashi Y., Nakahara K.S., Uyeda I.
    • Journal Title

      Archives Virology 153

      Pages: 105-115

    • Peer Reviewed
  • [Journal Article] A.Functional analysis of soybean genes involved in flavonoid biosynthesis by virus-induced gene silencing.2007

    • Author(s)
      Nagamatsu A., Masuta C., Senda M., Matsuura H., Kasai A., Hong J.-S., Kitamura K., Abe J., Kanazawa A.
    • Journal Title

      Plant Biotechnology Journal 5

      Pages: 778-90

    • Peer Reviewed
  • [Journal Article] Charactrization of silencing suppressor 2b of Cucumber mosaic virus based on examination of its small RNA-binding abilities.2007

    • Author(s)
      Goto K., Kobori T., Kosaka Y., Natsuaki T., Masuta C.
    • Journal Title

      Plant Cell Physiology 48

      Pages: 1050-1060

    • Peer Reviewed
  • [Journal Article] Genetic analysis of lethal tip necrosis induced by Clover yellow vein virus infection in pea.2007

    • Author(s)
      Ravelo G., Kagaya U., Inukai T., Sato M., Uyeda I.
    • Journal Title

      J.Gen.Plant Pathol. 73

      Pages: 59-65

    • Peer Reviewed
  • [Journal Article] Two resistance modes to Clover yellow vein virus in pea characterized by a green fluorescent protein-tagged virus.2007

    • Author(s)
      Andrade M., Sato M., Uyeda I.
    • Journal Title

      Phytoathology 97

      Pages: 544-550

    • Peer Reviewed
  • [Journal Article] Development of Cucumber mosaic virus as a vector modifiable for different host species to produce therapeutic proteins.2007

    • Author(s)
      Matsuo K., Hong J.-S., Tabayashi N., Ito A., Masuta C., Matsumura T.
    • Journal Title

      Planta 225

      Pages: 277-286

    • Peer Reviewed
  • [Journal Article] Rapid induction of transcriptional and post-transcriptional gene silencing using a novel Cucumber mosaic virus vector.2006

    • Author(s)
      Otagaki S., Arai M., Takahashi A., Goto K., Hong J.-S., Masuta C., Kanazawa, A.
    • Journal Title

      Plant Biotechnology 23

      Pages: 259-265

    • Peer Reviewed
  • [Presentation] Counterdefense against Viral RNAi Suppressors in Tobacco.2011

    • Author(s)
      中原健二, 他
    • Organizer
      Keystone Symposia on Molecular and Cellular Biology
    • Place of Presentation
      Monterey, California, USA
    • Year and Date
      20110322-20110325
  • [Presentation] キュウリモザイクウイルスYサテライトRNAが誘導する黄化症状の分子メカニズムの解析2011

    • Author(s)
      志村華子、増田税, 他
    • Organizer
      平成23年度日本植物病理学会大会
    • Place of Presentation
      府中市、東京農工大学
    • Year and Date
      2011-03-27
  • [Presentation] Effect of rgs-CaM overexpression on PVY accumulation in transgenic tobacco plants.2010

    • Author(s)
      Yamada S., Nakahara K., Uyeda I. 他
    • Organizer
      Keystone symposia (RNA silencing mechanisms inplants)
    • Place of Presentation
      Santa Fe, New Mexico, USA
    • Year and Date
      20100221-20100226
  • [Presentation] Screening of antiviral agents to inhibit RNA silencing suppressors of plant and animal viruses.2010

    • Author(s)
      Fujiwara A., Masuta C., 他
    • Organizer
      Antivirals Congress
    • Place of Presentation
      Amsterdam, Nederland
    • Year and Date
      2010-11-07
  • [Presentation] Cucumber mosaic virus vector has the ability to induce inheritable transcriptional gene silencing of endogenes in petunia.2009

    • Author(s)
      稲場純一、増田税, 他
    • Organizer
      The 1st Japan-Korea joint symposium
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      20091029-20091031
  • [Presentation] Two separated regions in virus genome contribute to high virulence expression of Clover yellow vein virus 90-1 Br-2 isolate in peas.2009

    • Author(s)
      Atsumi G., Nakahara K., Uyeda I. 他
    • Organizer
      The 1st Japan-Korea joint symposium
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      20091028-20091031
  • [Presentation] Comparison of the 2b proteins from two isolates of Tomato aspermy virus in silencing suppressor activity and viral systemic movement.2009

    • Author(s)
      Shimura H., Masuta C. 他
    • Organizer
      International Congress of Virology, The INTERNATIONAL UNION OF MICROBIOLOGICAL SOCIETIES
    • Place of Presentation
      Istanbul, ISTANBUL CONVENTION AND EXHIBITION CENTRE
    • Year and Date
      20090810-20090815
  • [Presentation] キュウリモザイクウイルスサテライトRNAが植物のRNAサイレンシングへ及ぼす影響の解析2009

    • Author(s)
      志村華子、増田税, 他
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形大学小白川キャンパス
    • Year and Date
      20090326-20090328
  • [Presentation] サブグループIとIIのキュウリモザイクウイルスの2bタンパク質のサイレンシング抑制活性の比較2009

    • Author(s)
      後藤一法、増田税, 他
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形大学小白川キャンパス
    • Year and Date
      20090326-20090328
  • [Presentation] キュウリモザイクウイルスサテライトRNAが植物のRNAサイレンシングへ及ぼす影響の解析2009

    • Author(s)
      志村華子、増田税, 他
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形市、山形大学
    • Year and Date
      20090326-20090328
  • [Presentation] サブグループIとIIのキュウリモザイクウイルスの2bタンパク質のサイレンシング抑制活性の比較2009

    • Author(s)
      後藤一法、増田税, 他
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形市、山形大学
    • Year and Date
      20090326-20090328
  • [Presentation] エンドウPI 226564系統においてクローバ葉脈黄化ウイルスの強毒性を決定するウイルス因子の探索2009

    • Author(s)
      厚見剛、中原健二、上田一郎, 他
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形市、山形大学
    • Year and Date
      20090326-20090328
  • [Presentation] クローバ葉脈黄化ウイルスはP1に点変異を生じることにより翻訳開始因子をコードするcyv-2遺伝子によるエンドウ劣性抵抗性を打破する2009

    • Author(s)
      中原健二、上田一郎, 他
    • Organizer
      平成21年度日本植物病理学会大会
    • Place of Presentation
      山形市、山形大学
    • Year and Date
      20090326-20090328
  • [Presentation] クローバ葉脈黄化ウイルスの病原性に関わる宿主タンパク質の同定2008

    • Author(s)
      山田翔太、中原健二、上田一郎, 他
    • Organizer
      日本ウイルス学会北海道支部 第42回夏期シンポジウム
    • Place of Presentation
      ニセコ町、ホテルニセコいこいの村
    • Year and Date
      20080726-20080727
  • [Presentation] RNAサイレンシングは植物組織の着色に必要なフラボノイド合成系遺伝子のmRNA量の閾値を明らかにする2008

    • Author(s)
      永松敦、増田税, 他
    • Organizer
      日本分子生物学会第31回年会
    • Place of Presentation
      神戸市、神戸ポートアイランド
    • Year and Date
      2008-12-12
  • [Presentation] クローバ葉脈黄化ウイルスの感染で誘導されるエンドウ遺伝子の発現2008

    • Author(s)
      山田翔太、中原健二、上田一郎, 他
    • Organizer
      平成20年度日本植物病理学会大会北海道部会
    • Place of Presentation
      札幌市、北海道農業研究センター
    • Year and Date
      2008-10-17
  • [Presentation] RNAサイレンシング抑制遺伝子による外来遺伝子の発現促進について2007

    • Author(s)
      中原健二、上田一郎, 他
    • Organizer
      第41回日本ウイルス学会北海道支部夏期シンポジウム
    • Place of Presentation
      小樽、かんぽの宿朝里荘
    • Year and Date
      20070823-20070824
  • [Presentation] プロトプラストを使用したウイルスのサイレンシングサプレッサーの機能解析2007

    • Author(s)
      志村華子、増田税, 他
    • Organizer
      平成19年度日本植物病理学会大会
    • Place of Presentation
      宇都宮、宇都宮大学農学部
    • Year and Date
      20070328-20070330
  • [Presentation] エンドウにおいてクローバー葉脈黄化ウイルスが誘導する全身性の細胞死とRNA silencing suppressor活性の関係2007

    • Author(s)
      厚見剛、中原健二、上田一郎, 他
    • Organizer
      平成19年度日本植物病理学会大会
    • Place of Presentation
      宇都宮、宇都宮大学農学部
    • Year and Date
      20070328-20070330
  • [Presentation] エンドウにおけるクローバー葉脈黄化ウイルスの病原性にはRNA silencing suppressor活性が関与する2007

    • Author(s)
      厚見剛、中原健二、上田一郎, 他
    • Organizer
      第55回日本ウイルス学会
    • Place of Presentation
      札幌、札幌コンベンションセンター
    • Year and Date
      2007-10-22
  • [Presentation] キュウリモザイクウイルスベクターを用いたペチュニアの内在性遺伝子プロモーターへのメチル化誘導2007

    • Author(s)
      稲場純一、増田税, 他
    • Organizer
      平成19年度日本植物病理学会北海道部会
    • Place of Presentation
      札幌、北方圏センター
    • Year and Date
      2007-10-12
  • [Presentation] キュウリモザイクウイルスされライトRNAは宿主RNAサイレンシングを促進する2007

    • Author(s)
      小暮勇樹、増田税, 他
    • Organizer
      平成19年度日本植物病理学会北海道部会
    • Place of Presentation
      札幌、北方圏センター
    • Year and Date
      2007-10-12
  • [Presentation] Clover yellow vein virus expresses virulence by salicylic acid-mediated pathway in pea.2007

    • Author(s)
      Atsumi G., Nakahara S., Uyeda I. 他
    • Organizer
      XIII International Congress on Molecular Plant-Microbe Interactions
    • Place of Presentation
      イタリア、ソレント、ホテルヒルトン
    • Year and Date
      2007-07-25
  • [Remarks] ホームページ

    • URL

      http://www.agr.hokudai.ac.jp/ikushu/byougen/saito/purojekuto_yan_jiu.html

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Published: 2012-01-26   Modified: 2014-06-30  

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