2010 Fiscal Year Final Research Report
Remodeling on molecular mechanisms of pregnancy establishment and regulation
| Project/Area Number |
18108004
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IMAKAWA Kazuhiko The University of Tokyo, 大学院・農学生命科学研究科, 准教授 (00291956)
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| Co-Investigator(Renkei-kenkyūsha) |
TANAKA Tomo 東京大学, 大学院・農学生命科学研究科, 准教授 (90242164)
MANABE Noboru 東京大学, 大学院・農学生命科学研究科, 教授 (80243070)
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| Project Period (FY) |
2006 – 2010
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| Keywords | 動物 / 遺伝子 / 発現制御 / 妊娠 / リモデリング / インターフェロン / トロホブラスト細胞 / 着床 |
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| Research Abstract |
The goal of this study is to elucidate molecular mechanisms by which pregnancy is established in mammalian species. Among several bovine interferon tau (IFNT) genes, only two of them were expressed in vivo, one of IFNTs induced Mx2expression by endometrial cells. Addition of uterine flushing media mimicked the expression of IFNT and CDX2/EOMES transcription factors in the endometrial and trophoblast CT-1co-culture system. In the bovine uterus, attachment of elongated conceptus to the maternal epithelium induced changes in epigenetic status of IFNT gene: reduction in H3 acetylation and increase in H3 methylation. In addition, the conceptus expresses mesenchymal genes while keeping epithelial cell nature. Furthermore, several novel genes were identified during the pre-attachment period, which could be used to detect early pregnancy in the bovine species. Through these experimentations, novel mechanisms regulating pregnancy establishment could be established.
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[Journal Article] Quantitative analysis of retromer complex-related genes during embryo development in the mouse.2011
Author(s)
Park S-J, Huh J-W, Kim Y-H, Kim J-S, Song B-S, Kim S-H, Kim E, Lee S-R, Kim S-U, Kim H-S, Imakawa K, Chang K-T
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Journal Title
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[Journal Article] Induction of endogenous tau interferon gene transcription by CDX2 and high acetylation in bovine non-rophoblast cells.2009
Author(s)
Sakurai T, Sakamoto A, Muroi Y, Bai H, Nagaoka K, Tamura K, Takahashi T, Hashizume K, Sakatani M, Takahashi M, Godkin JD, Imakawa K.
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Journal Title
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[Journal Article] Up-regulation of NOD1 and NOD2 through TLR4 and TNF-alpha in LPS-treated murine macrophages.2006
Author(s)
Takahashi T, Isuzugawa K, Murase Y, Imai M, Yamamoto S, Iizuka M, Akira S, Bahr GM, Momotani E, Hori M, Ozaki H, Imakawa K.
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Journal Title
J.Vet.Med.Sci. 68
Pages: 471-478
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