2009 Fiscal Year Final Research Report
Design of Base-discriminating Fluorescent Nucleosides and Their Application to Gene Analysis and SNP Genotyping
| Project/Area Number |
18201026
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Nihon University |
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Principal Investigator |
SAITO Isao Nihon University, 工学部, 教授 (20026082)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Yoshio 日本大学, 工学部, 准教授 (40385985)
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| Project Period (FY) |
2006 – 2009
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| Keywords | DNAデバイス / DNAチップ / 遺伝子検出 / 遺伝子診断 / SNP検出 |
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| Research Abstract |
We have designed novel base-discriminating fluorescent (BDF) nucleosides which are successfully used for SNP genotyping and gene detection. Quencher and end free molecular beacons (MB) were discovered for the first time. These low cost MB were used for the gene detection in a cell. We have also succeeded to design environmentally sensitive fluorescent nucleosides that emits strong fluorescence at different wavelengths depending upon a local environment around the nucleobase.
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