Towards comprehensive analysis of genetic interaction of E. coli

2008 Fiscal Year Final Research Report

• Project/Area Number: 18201038
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 基礎ゲノム科学
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: MORI Hirotada Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (90182203)
• Research Collaborator: BARRY L. Wanner Purdue University, 教授
• Project Period (FY): 2006 – 2008
• Keywords: 遺伝的相互作用 / 遺伝的ネットワーク / geneticinteraction / 合成致死 / 大腸菌 / 接合 / バーコード

Research Abstract

網羅的に遺伝的ネットワークを解明することを目的に、2重欠失株作製の系の開発と解析の評価を行った. 2重欠失の為の既存の欠失株ライブラリーにさらにもう1種類の欠失株ライブラリーの構築、単一欠失の接合による2重化のツールの開発、2重化のhighthroughput化、解析システムのそれぞれの開発を行った. 新規欠失株ライブラリーには、20ntのbarcodeを挿入し、創薬等のhighthroughputスクリーニングへの道も開いた.

Research Products

• [Journal Article] High-throughput (2008)
  • Author(s): Typas, A., R.J. Nichols, D.A. Siegele, M.Shales, S.R. Collins, B. Lim, H. Braberg, N.Yamamoto, R. Takeuchi, B.L. Wanner, H. Mori, J.S. Weissman, N.J. Krogan, and C.A. Gross
  • Journal Title: quantitative analyses of genetic interactions in E. coli. Nat Methods 5 Pages: 781-7
  • Peer Reviewed
• [Journal Article] J.F. Greenblatt, and A. Emili. eSGA : E (2008)
  • Author(s): Butland, G., M. Babu, J.J. Diaz-Mejia, F.Bohdana, S. Phanse, B. Gold, W. Yang, J.Li, A.G. Gagarinova, O. Pogoutse, H. Mori, B . L . Wa n n e r , H.Lo, J.Wasniewski, C.Christopolous, M. Ali, P. Venn, A. Safavi-Naini, N. Sourour, S. Caron, J.Y. Choi, L. Laigle, A. Nazarians-Armavil, A. Deshpande, S. Joe, K.A. Datsenko, N. Yamamoto, B.J. Andrews,C. Boone, H. Ding, B. Sheikh, G. Moreno-Hagelseib
  • Journal Title: coli synthetic genetic array analysis. Nat Methods 5 Pages: 789-95
  • Peer Reviewed
• [Presentation] Systems Biology of E. coli, Institute of Biomedical Sciences (2007)
  • Author(s): Mori Hirotda
  • Organizer: Fudan Universityほか3件
  • Place of Presentation: Shanghai, Republic of China
  • Year and Date: 2007-11-16
• [Presentation] Systems Approach Towards Understanding of E. coli Central Metabolism-Quantiatative Measerement of Transcription and Translation of Glycolytic and TCA Cycle Enzymes (2007)
  • Author(s): Mori Hirotada
  • Organizer: 2nd ASM Conference on Integrating Metabolism and Genomics(IMAGE2)ほか28件
  • Place of Presentation: Quebec, Canada
  • Year and Date: 2007-05-03
• [Presentation] Datsenko K.A.&Wanner B.L., Systems approach towards understanding of E (2007)
  • Author(s): Mori H., Dose H., Ikegami Y., Yamamotoya T.,Matsuno H., Narifusa Y., Nakahigashi K., Baba T.
  • Organizer: coli central metabolism- Quantitative measurement of transcription and translation of glycolytic and TCA cycle enzymes, IMAGE2(30-03)
  • Place of Presentation: Quebec, Canada
  • Year and Date: 2007-04-05
• [Presentation] 大腸菌のシステム生物学
  • Author(s): 森浩禎
  • Organizer: 21世紀大腸ほか28件
  • Place of Presentation: ポスター発表
• [Book] Single-Gene Knockout Mutant Collection in Escherichia coli K-12. Methods Mol Biol ; 416 (2008)
  • Author(s): Baba, T. and H. Mori
  • Total Pages: 171-81
  • Publisher: The Construction of Systematic In-Frame
• [Book] Single-Gene Knockout Mutant Collection of Escherichia coli K-12. Methods Mol Biol ; 416 (2008)
  • Author(s): Baba, T., H.C. Huan, K. Datsenko, B.L. Wanner, and H. Mori
  • Total Pages: 183-94
  • Publisher: The Applications of Systematic In-Frame
• [Remarks] データベース
  • URL: http://ecoli.naist.jp