Anisotropic dynamics of ice growth promotion and its self-oscillation induced by the interfacial adsorption of biological macro molecules

2009 Fiscal Year Final Research Report

• Project/Area Number: 18204036
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics/Chemical physics
• Research Institution: Hokkaido University
• Principal Investigator: FURUKAWA Yoshinori Hokkaido University, 低温科学研究所, 教授 (20113623)
• Co-Investigator(Kenkyū-buntansha): SAZAKI Gen 北海道大学, 低温科学研究所, 准教授 (60261509)
• Co-Investigator(Renkei-kenkyūsha): KATAGIRI Chihiro 北海道大学, 低温科学研究所, 助教 (90002245) ; YOKOYAMA Etsuro 学習院大学, 計算機センター, 教授 (40212302) ; NADA Hiroki 独立行政法人産業技術総合研究所, 環境管理研究部門, 主任研究員 (90357682) ; KANEKO Fumitoshi 大阪大学, 大学院・理学(系)研究科, 准教授 (70214468)
• Project Period (FY): 2006 – 2009
• Keywords: 生物物理

Research Abstract

Experimental and theoretical studies of antifreeze proteins, which have the special function to prevent the freezing of living organisms under the subzero environment, were carried out. Direct evidence for the relation between the adsorption of proteins on the ice interfaces and the prohibition of crystal growth was first demonstrated directly, and it was made clear that the protein molecule adsorption proceeded in two stages depending on the conformation change of protein molecules. This adsorption mode was completely new and called as "two-step reversible adsorption". This adsorption model also explained the growth promotion of basal planes of ice and the self oscillations of growth rates.

Research Products

• [Journal Article] 氷結晶成長を支配するタンパク質-氷点下に生きる生物はいかにして凍結を回避するか- (2010)
  • Author(s): 古川義純, Salvador Zepeda, 宇田幸弘
  • Journal Title: 日本物理学会誌 65 Pages: 98-103
  • Peer Reviewed
• [Journal Article] Apparatus for single ice crystal growth from the melt (2009)
  • Author(s): Salvador Zepeda, Shunichi Nakatsubo, Yoshinori Furukawa
  • Journal Title: Rev. Sci. Inst. 80 Pages: 115102
  • Peer Reviewed
• [Journal Article] Growth of ice disk: Dependence of critical thickness for ice disk instability on supercooling of water. (2009)
  • Author(s): E. Yokoyama, R.F. Sekerka, Y. Furukawa
  • Journal Title: Journal of Physical Chemistry B113 Pages: 4733-4738
  • Peer Reviewed
• [Journal Article] Directly probing the antifreeze protein kinetics at the ice/solution interface. (2008)
  • Author(s): Salvador Zepeda, Yukihiro Uda, Yoshinori Furukawa
  • Journal Title: ournal of Japanese Society for Crystal Growth 35 Pages: 151-160
  • Peer Reviewed
• [Journal Article] In-situ observation of antifreeze glycoprotein kinetics at the ice surface reveals a two-step reversible adsorption mechanism (2008)
  • Author(s): Zepeda, Salvador, Yokoyama, Etsuro, Uda, Yukihiro, Katagiri, Chihiro, Furukawa, Yoshinori
  • Journal Title: Crystal Growth & Design 8 Pages: 3666-3672
  • Peer Reviewed
• [Journal Article] Growth inhibition mechanism of an ice-water interface by a mutant of winter flounder antifreeze protein: a molecular dynamics study (2008)
  • Author(s): Hiroki Nada, Yoshinori Furukawa
  • Journal Title: Journal of Physical Chemistry B112 Pages: 7111-7119
  • Peer Reviewed
• [Journal Article] Adsorption-Induced Conformational Changes of Antifreeze Glycoproteins at the Ice/Water Interface (2007)
  • Author(s): Yukihiro Uda, Salvador Zepeda, Fumitoshi Kaneko, Yoshiki Matsuura, Yoshinori Furukawa
  • Journal Title: Journal of Physical Chemistry B111 Pages: 14335-14361
  • Peer Reviewed
• [Journal Article] Growth kinetics on interface between (2021) plane of ice and water investigated by a molecular dynamics simulation (2007)
  • Author(s): H. Nada, Y. Furukawa
  • Journal Title: Proceedings on International Conference on Physics and Chemistry of Ice Pages: 443-450
  • Peer Reviewed
• [Journal Article] Diffusion, incorporation, and segregation of antifreeze proteins at the ice/solution interface (2007)
  • Author(s): S. Zepeda, H. Nakaya, Y. Uda, E. Yokoyama, Y. Furukawa
  • Journal Title: Proceedings on International Conference on Physics and Chemistry of Ice Pages: 669-676
  • Peer Reviewed
• [Presentation] Ice Crystal Growth-From Space Experiment to Biological Aspect. (2009)
  • Author(s): Y. Furukawa
  • Organizer: The 3rd UT Horiba International Symposium and The 11th ISSP International Symposium (ISSP-11) on Hydrogen and Water in Condensed Matter Physics
  • Place of Presentation: Chiba(Plenary lecture)
  • Year and Date: 2009-10-20
• [Presentation] Antifreeze (glyco-) proteins- How do they affect ice crystal growth? (2009)
  • Author(s): Y. Furukawa
  • Organizer: 17th American Conference on Crystal Growth and Epitaxy (ACCGE-17)
  • Place of Presentation: Lake Geneva, WI(Invited lecture)
  • Year and Date: 2009-08-12
• [Presentation] Ice crystal growth-from space experiment to antifreeze- (2009)
  • Author(s): Y. Furukawa
  • Organizer: 46th Annual Meeting of the Society for Cryobiology (CRYO2009)
  • Place of Presentation: Sapporo(Plenary lecture)
  • Year and Date: 2009-07-20
• [Presentation] Reversible adsorption of antifreeze glycoproteins and ice growth (2008)
  • Author(s): Y. Furukawa
  • Organizer: NORDITA Symposium on Ice & Water in the Universe: from Astrobiology to Terrestrial Bodies
  • Place of Presentation: Stockholm(Invited lecture)
  • Year and Date: 2008-09-23
• [Presentation] Behavior of antifreeze protein and glycoprotein at the ice/solution interface and ice growth kinetics during one-directional growth (2007)
  • Author(s): Furukawa, Y., Zepeda, S., Nakaya, H., Uda, Y., Yokoyama, E.
  • Organizer: American Chemical Society Fall 2007 National Meeting&Exposition
  • Place of Presentation: Boston
  • Year and Date: 2007-08-20
• [Presentation] Diffusion, Adsorption and Incorporation of Antifreeze Protein Molecules at Growing Ice Interface (2007)
  • Author(s): Y. Furukawa
  • Organizer: Graduate School Initiative Young Brains 21 Program-:Frontier of Crystal Growth Science (Tohoku Univ. )
  • Place of Presentation: Sendai(Invited lecture)
  • Year and Date: 2007-02-10
• [Book] Effects of additives on ice crystal growth in supercooled water, Recent Development of Chemistry and Photochemistry in Ice, Ed N. Takenaka, (2008)
  • Author(s): Y. Furukawa, K. Nagashima
  • Total Pages: 27-48
  • Publisher: Transworld Research Network
• [Remarks] 北海道新聞2009年1月7日「研究室探訪:自然現象を読み解く鍵」
• [Remarks] Zepeda Salvador:日本結晶成長学会奨励賞「不凍タンパク質の氷/水界面吸着と氷結晶成長抑制機構の解明」(2009年11月13日受賞)
• [Remarks] 灘浩樹:日本結晶成長学会論文賞「新しい水分子ポテンシャルモデルの開発とこれに基づく一連の氷結晶成長機構の分子レベルの解明」(2008年11月11日受賞)