2009 Fiscal Year Final Research Report
Development of New Chemistry-based Methods for Protein Modification and Engineering That can be Applied under Crude Cellular Conditions
| Project/Area Number |
18205020
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyoto University |
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Principal Investigator |
HAMACHI Itaru Kyoto University, 大学院・工学研究科, 教授 (90202259)
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| Co-Investigator(Kenkyū-buntansha) |
OJIDA Akio 京都大学, 大学院・工学研究科, 講師
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| Project Period (FY) |
2006 – 2009
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| Keywords | 細目表・生物有機化学 / 細目表以外・ケミカルパイオロジー |
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| Research Abstract |
Chemistry-based selective labeling of an intended target protein under very complicated crude systems like cell, tissues or in vivo is an important and challenging project in chemical biology for understanding and controlling the protein functions under such really working conditions. However, it is absolutely difficult because of many obstacles. My group in this research grant successful developed three distinct and new methods to label and modify a protein of interest under crude conditions like cell lysates, live cell or in vivo by elaborately combining the sophisticated molecular recognition events with orthogonal and selective organic reactions applicable even in aqueous medium.
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[Journal Article] Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound2009
Author(s)
S. Kiyonaka, K. Kato, M. Nishida, K. Mio, T. Numaga, Y. Sawaguchi, T. Yoshida, M. Wakamori, E. Mori, T. Numata, M. Ishii, H. Takemoto, A. Ojida, K. Watanabe, A. Uemura, H. Kurose, T. Morii, T. Kobayashi, Y. Sata, C. Sato, I. Hamachi, Y. Mori
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Journal Title
Proc. Natl. Acad. Sci. USA 106
Pages: 5400-5405
Peer Reviewed
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[Journal Article] Molecular Characterization of Flubendiamide Sensitivity in the Lepidopterous Ryanodine Receptor Ca2+ Release2009
Author(s)
K. Kato, S. Kiyonaka, Y. Sawaguchi, M. Tohnishi, T. Masaki, N. Yasokawa, Y. Mizuno, E. Mori, K. Inoue, I. Hamachi, H. Takeshima, Y. Mori
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Journal Title
Channel Biochemistry 48
Pages: 10342-10352
Peer Reviewed
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