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2009 Fiscal Year Annual Research Report

シロイヌナズナにおける形態形成と耐病性のクロストークに関する分子遺伝学的研究

Research Project

Project/Area Number 18207003
Research InstitutionNara Institute of Science and Technology

Principal Investigator

田坂 昌生  Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (90179680)

Co-Investigator(Kenkyū-buntansha) 打田 直行  奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40467692)
Keywordsシロイヌナズナ / CC-NB-LRR / 茎頂メリステム / UNI遺伝子 / サプレッサー / 相互作用因子
Research Abstract

これまで、茎頂分裂組織(SAM)および腋生分裂組織(AM)にユニークな異常を示すuni-1D変異体の解析を通じて、分裂組織の全く新しい制御機構の発見を目指した研究を行ってきた。本年度の成果は以下の通りである。ERECTA(ER)受容体キナーゼの機能欠損によりuni-1D変異体のSAMの異常が回復した。また、uni-ID変異体のSAMの異常にはSAM外部でのERの機能が重要であった。さらに、ERファミリー因子群の機能をすべて欠損させると、uni-1D変異体のAMの異常もまた抑圧された。これらを踏まえた解析の結果、野生型背景においても、分裂組織外でのERファミリーの機能が分裂組織の制御に関わることが示唆された。また、UNIタンパク質のCCドメインに結合する可能性が高いタンパク質として、26Sプロテアソームの構成タンパク質の一つであるRPT2aを酵母2ハオブリッド法で同定した。rpt2a変異体とuni-1Dの2重変異体を作成し解析した結果、rpt2a変異がuni-1D変異をサプレッスする事が明らかになった。この結果から、UNIとRPT2aはタンパク質相互作用を行いつつ協調して信号伝達を行う可能性が示唆された。野生型のUNIタンパク質を過剰発現すると恒常活性型の優性変異を持つuni-1Dと同じ表現型を示す。これを明らかにした形質転換植物の解析から、UNI遺伝子の3'UTRがタンパク質の生産性の向上に関連する事も明らかになった。

  • Research Products

    (26 results)

All 2010 2009 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (18 results) Remarks (1 results)

  • [Journal Article] Loss-of-function mutations of retromer large subunits suppress the phenotype of zig mutant that lacks Qb-SNARE VTI112010

    • Author(s)
      Hashiguchi Y., Niihama M., Takahashi T., Saito C., Nakano A., Tasaka M., Monita M.T.
    • Journal Title

      Plant Cell 22

      Pages: 159-172

    • Peer Reviewed
  • [Journal Article] Role of auxin efflux in apical hook development of Arabidopsis thaliana2010

    • Author(s)
      Zadnikova P., Petasek J., Marhavy P., Raz V., Vandenbussche F., Ding Z, Morita M.T., Tasaka M., Straeten D.V.D., Friml J., Benkova E.
    • Journal Title

      Development. 37

      Pages: 607-617

    • Peer Reviewed
  • [Journal Article] Arole for Arabidopsis PUCHI in floral meristem identity and bract suppression2009

    • Author(s)
      Karim M.R., Hirota k, kwiatkowska D., Tasaka M., Aida M.
    • Journal Title

      Plant Cell 21

      Pages: 1360-1372

    • Peer Reviewed
  • [Journal Article] Defects in dynamics and functions of actin filament in Arabidopsis caused by the dominant-negative actin fiz-1-induced fragmentation of actin filament2009

    • Author(s)
      Kato t., Monta.T.M.TasakaM.
    • Journal Title

      Plant Cell Physiol. 51

      Pages: 333-338

    • Peer Reviewed
  • [Journal Article] ZIP genes encode proteins involved in membrane trafficking of the TGN-PVC/vacuoles.2009

    • Author(s)
      Niihama M., Takemoto N., Hashiguchi Y., Tasaka M., Morita M.T.
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 2057-2068

    • Peer Reviewed
  • [Journal Article] Dynamic aspects of ion accumulation by vesicle traffic under salt stress in Arabidopsis2009

    • Author(s)
      Hamaji K., Nagira M., Yoshida K., Ohnishi M., Oda Y., Uemura T., Goh T., Sato M.H., Morita M.T., Tasaka M., Hasezawa S., Nakano A., Nishimura I.H., Maeshima M., Fukaki H., Mimura T.
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 2013-2033

    • Peer Reviewed
  • [Journal Article] Hormone interactions during lateral root formation2009

    • Author(s)
      Fukaki, H., Tasaka M.
    • Journal Title

      Plant Mol.Biol. 69

      Pages: 437-449

    • Peer Reviewed
  • [Presentation] 茎頂分裂組織か"肥大化するSHOOT MERISTEMLESS新奇アリルgorgonの解析2010

    • Author(s)
      Sho Takano, Mitsuru Niihama, Masao Tasaka, Mitsuhiro Aida
    • Organizer
      日本植物生理学会第51回大会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-21
  • [Presentation] 分裂組織外でのERECTAファミリーの機能が分裂組織に与える影響の解析2010

    • Author(s)
      Naoyuki Uchida, Masao Tasaka
    • Organizer
      日本植物生理学会第51回大会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-20
  • [Presentation] NPH3様タンパク質MAB4およびMELはPINタンパク質の局在を制御する2010

    • Author(s)
      Masahiko Furutani, Shuhei Yoshida, Norihito Sakamoto, Masao Tasaka
    • Organizer
      日本植物生理学会第51回大会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-20
  • [Presentation] Ring type E3 ligase SGR9は重力受容に重要なアミロプラスト動態制御に関与する2010

    • Author(s)
      森田(寺尾)美代, 中村守貴, 田坂昌生
    • Organizer
      日本植物生理学会第51回大会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-20
  • [Presentation] Molecular genetic study of gravity perception in Arabidopsis shoots2009

    • Author(s)
      Masao Tasaka
    • Organizer
      Memorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
    • Place of Presentation
      京都
    • Year and Date
      2009-12-03
  • [Presentation] Analysis of amyloplast dynamics involved in gravity sensing using a novel centrifuge microscope2009

    • Author(s)
      Masatsugu Toyota, Masao Tasaka, Miyo Terao Morita
    • Organizer
      Manorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
    • Place of Presentation
      京都
    • Year and Date
      2009-12-02
  • [Presentation] Polar localized NPH3-like proteins MEL regulate auxin- related morphogenesis by the control of PIN localization2009

    • Author(s)
      Masahiko Furutani, Shuhei Yoshida, Takahito Kajiwara, Masao Tasaka
    • Organizer
      Memorial Symposium for the 25th International Prize for Biology "Biology of Sensing"
    • Place of Presentation
      京都
    • Year and Date
      2009-12-02
  • [Presentation] シロイヌナズナ重力受容における液胞機能に関わるPLA1様蛋白質SGR2の解析2009

    • Author(s)
      森田(寺尾)美代、安藤鮎美、倉増紀代子、加藤壮英、田坂昌生
    • Organizer
      第82回日本生化学会
    • Place of Presentation
      兵庫
    • Year and Date
      2009-10-22
  • [Presentation] MAB4/ENP family proteins involved in auxin-regulated morphogenesis in Arabidopsis2009

    • Author(s)
      Tasaka M.
    • Organizer
      The 9th International Plant Molecular Biology
    • Place of Presentation
      St. Louis USA
    • Year and Date
      2009-10-06
  • [Presentation] CC-NB-LRRの活性化が茎頂分裂組織に影響を与える機構の解析2009

    • Author(s)
      Naoyuki Uchida, Kadunan Igari, Masao Tasaka
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      山形
    • Year and Date
      2009-09-18
  • [Presentation] Analysis of influences of CC-NB-LRR-related signaling on meristem regulation2009

    • Author(s)
      Uchida N
    • Organizer
      Japan-German Symposium on Evolution and Development
    • Place of Presentation
      Cologne Germany
    • Year and Date
      2009-08-25
  • [Presentation] The MAB4/ENP family genes involved in auxin-regulated morphogenesis2009

    • Author(s)
      Masahiko Furutani, Shuhei Yoshida, Masao Tasaka
    • Organizer
      The 20th International Conference on Arabklopsis Research
    • Place of Presentation
      Edinburgh UK
    • Year and Date
      2009-07-25
  • [Presentation] SHOOT GRAVTTROPISM 9, a novel RING finger protein, is involved in statolith dynamics by modulating interaction between F-actin and amyloplasts.2009

    • Author(s)
      Miyo T.Morita, Moritaka Nakamura, Masao Tasaka
    • Organizer
      Plant Biology 2009
    • Place of Presentation
      Hawaii USA
    • Year and Date
      2009-07-21
  • [Presentation] The MAB4/ENP family genes involved in auxin-regulated morphogenesis2009

    • Author(s)
      Masahiko Funitani, Shuhei Yoshida, Masao Tasaka
    • Organizer
      ACPD 2009
    • Place of Presentation
      Prague Czech Republic
    • Year and Date
      2009-07-11
  • [Presentation] Control mechanisms for activation of a novel CC-NBS-LRR protein, UNI-mediated signals that induce both SA-dependent defense and CK-dependent morphological signals2009

    • Author(s)
      Kwi Mi Chung, Kadunari Igari, Masao Tasaka
    • Organizer
      The 20th International Conference on Arabidopsis Research
    • Place of Presentation
      Edinburgh UK
    • Year and Date
      2009-07-02
  • [Presentation] Investigation of mechanism for gravity sensing in Arabidopsis inflorescence stems using a novel centrifuge microscope2009

    • Author(s)
      Masatsugu Toyota, Masao Thsaka, Miyo Terao Morita
    • Organizer
      Plant Biology 2009
    • Place of Presentation
      Hawaii USA
    • Year and Date
      2009-07-02
  • [Presentation] The MAB4/ENP family genes involved in auxin-regulated morphogenesis2009

    • Author(s)
      Masahiko Fumtani, Shuhei Yoshida, Masao Tasaka
    • Organizer
      Society for Developmental Bioligy 68th Annual Meeting
    • Place of Presentation
      California USA
    • Year and Date
      2009-07-01
  • [Presentation] Signaling triggered by activation of CC-NB-LRR-related UNI affects SAM activity in anon-cell-autonomous manner involving ERECTA receptor kinase2009

    • Author(s)
      Naoyukd Uchida, Kadunari Igari, Masao Tasaka
    • Organizer
      The 20th International Conference on Arabidopsis Research
    • Place of Presentation
      Edinburgh UK
    • Year and Date
      2009-07-01
  • [Remarks]

    • URL

      http://bsw3.naist.jp/keihatsu/keihatsu.html

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Published: 2011-06-16   Modified: 2016-04-21  

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