2009 Fiscal Year Final Research Report
Study on the structure and funciotion of supramolecular flagellar motor
| Project/Area Number |
18207011
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
HOMMA Michio Nagoya University, 大学院・理学研究科, 教授 (50209342)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Seiji 名古屋大学, 大学院・理学研究科, 助教 (70420362)
|
|---|
| Project Period (FY) |
2006 – 2009
|
| Keywords | べん毛 / エネルギー変換 / メンブレン / ナノバイオ / 分子モーター |
|---|
| Research Abstract |
The proteins, PomA, PomB, MotX and MotY have been identified as the motor proteins that are necessary for the flagella rotation. The T ring newly found by us is necessary for the assembly. The structure of the component protein, MotY, of the T ring is determined with a 2.9 Å resolution. We proposed the molecule dynamic model of the flagella motor proteins to assemble as a functional motor. We showed that the C ring structure was easily dissociated from the flagella base body. Furthermore we found that FlgT was the basal body component to construct a ring outside of the LP ring. The ring was named an H ring. The new ring may contribute on the Vibro motor function as a strong shaft to support the high-speed rotation of the motor.
|
-
-
-
[Journal Article] Stator assembly and activation mechanism of the flagellar motor by the periplasmic region of MotB.2009
Author(s)
Kojima, S., Imada, K., Sakuma, M., Sudo, Y., Kojima, C., Minamino, T., Homma, M., Namba, K.
-
Journal Title
Mol. Micro. 73(4)
Pages: 710-718
Peer Reviewed
-
-
-
-
-
[Journal Article] Systematic Cys mutagenesis of Flgl, the flagellar P-ring component of Escherichia coli.2008
Author(s)
Hizukuri, Y., Kojima, S., Yakushi, T., Kawagishi, I., Homma, M.
-
Journal Title
Microbiology 154(3)
Pages: 810-817
Peer Reviewed
-
[Journal Article] Insights into the stator assembly of the Vibrio flagellar motor from the crystal structure of MotY.2008
Author(s)
Kojima, S., Shinohara, A., Terashima, H., Yakushi, T., Sakuma, M., Homma, M., Namba, K., Imada, K.
-
Journal Title
Proc. Nat. l Acad. Sci. USA. 105(22)
Pages: 7696-7701
Peer Reviewed
-
[Journal Article] Crystallization and preliminary X-ray analysis of MotY, a stator component of the Vibrio alginolyticus polar flagellar motor.2007
Author(s)
Shinohara, A., Sakuma, M., Yakushi, T., Kojima, S., Namba, K., Homma, M., Imada, K.
-
Journal Title
Acta. Cryst. 63(2)
Pages: 89-92
Peer Reviewed
-
-
-
[Journal Article] Roles of the intramolecular disulfide bridge in MotX and MotY, the specific proteins for sodium-driven motors in Vibrio spp.2006
Author(s)
Yagasaki, J., Okabe, M., Kurebayashi, R., Yakushi, T., Homma, M.
-
Journal Title
I Bacteriol. 188(14)
Pages: 5308-5314
Peer Reviewed
-
-
[Journal Article] The Vibrio motor proteins, MotX and MotY, are associated with the basal body of Na+-driven flagella and required for stator formation2006
Author(s)
Terashima, H., Fukuoka, H., Yakushi, T., Kojima, S., Homma, M.
-
Journal Title
M. Mol. Micro. 62(4)
Pages: 1170-1180
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
