Integrated studies on signaling and physiological function of green tea catechin through the catechin receptor

2009 Fiscal Year Final Research Report

• Project/Area Number: 18208012
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Kyushu University
• Principal Investigator: TACHIBANA Hirofumi Kyushu University, 大学院・農学研究院, 准教授 (70236545)
• Co-Investigator(Kenkyū-buntansha): FUJIMURA Yoshinori 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (20390304)
• Project Period (FY): 2006 – 2009
• Keywords: EGCG / 緑茶 / 受容体 / シグナリング / 67LR / カテキン / 機能性

Research Abstract

67-kDa laminin receptor (67LR) has been identified as a cell surface receptor for green tea polyphenol (-)-epigallocatechin-3-gallate(EGCG)that mediates the anticancer activity of EGCG. Eukaryotic translation elongation factor 1A (eEF1A) and myosin phosphatase targeting subunit 1(MYPT1) have been identified as components responsible for the anticancer activity of EGCG in vivo. We identified functional motif on the extracellular domain of 67LR for exerting EGCG action. We also found that 67LR mediates anti-allergic and anti-inflammatory activities of EGCG, and clarified the molecular basis for the EGCG action though the receptor.

Research Products

• [Journal Article] Green tea polyphenol epigallocatechin-3-gallate signaling pathway through 67-kDa laminin receptor (2008)
  • Author(s): Umeda, D., Yano, S., Yamada, K., Tachibana, H.
  • Journal Title: J. Biol. Chem. 283 Pages: 3050-3058
  • Peer Reviewed
• [Journal Article] The impact of the 67 kDa laminin receptor on both cell-surface binding and anti-allergic action of tea catechins (2008)
  • Author(s): Fujimura, Y., Umeda, D., Yamada, K., Tachibana, H
  • Journal Title: Arch. Biochem. Biophys 476 Pages: 133-138
  • Peer Reviewed
• [Journal Article] The 67kDa laminin receptor as a primary determinant of anti-allergic effects of O-methylated EGCG (2007)
  • Author(s): Fujimura, Y., Umeda, D., Yano, S., Maeda-Yamamoto, M., Yamada, K., Tachibana, H.
  • Journal Title: Biochem. Biophys. Res. Commun. 364 Pages: 79-85
  • Peer Reviewed
• [Journal Article] TLR 4 signaling inhibitory pathway induced by green tea polyphenol epigallocatechin 3 gallate through 67-kDa laminin receptor
  • Author(s): Byun, E.H., Fujimura, Y., Yamada, K., Tachibana, H.
  • Journal Title: J. Immunol. (in press)
  • Peer Reviewed
• [Presentation] 緑茶カテキンのケミカルバイオロジー (2009)
  • Author(s): 立花宏文
  • Organizer: 日本カテキン学会基調講演
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-09
• [Presentation] 緑茶カテキンの標的分子研究からみえてきたこと (2009)
  • Author(s): 立花宏文
  • Organizer: 日本農芸化学会大会シンポジウム
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-03-28
• [Presentation] 緑茶カテキン受容体67LRを介したカテキンの機能性発現機構 (2008)
  • Author(s): 立花宏文
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-03-17
• [Presentation] Green tea polyphenol EGCG signaling through the 67kDa laminin receptor (2007)
  • Author(s): Tachibana, H.
  • Organizer: 3rd International Conference on Polyphenols and Health
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2007-11-28
• [Book] Food Factors for Health Promotion (2009)
  • Author(s): Tachibana, H., (Yoshikawa, T. ed.)
  • Total Pages: 156-169
• [Book] 栄養学研究の最前線 (2008)
  • Author(s): 立花宏文(小川正、河田照雄、寺尾純二編集)
  • Total Pages: 93-107
• [Book] 食品機能性の科学 (2008)
  • Author(s): 立花宏文(食品機能性の科学編集委員会編集)
  • Total Pages: 323-327
• [Patent(Industrial Property Rights)] エピガロカテキンガレート誘導体及びそれを含む医薬組成物 (2010)
  • Inventor(s): 立花宏文, 高橋孝志, 田中浩二
  • Industrial Property Rights Holder: 国立大学法人九州大学,国立大学法人東京工業大学
  • Industrial Property Number: 特許・特願2010-028974
  • Filing Date: 2010-02-12
• [Patent(Industrial Property Rights)] ガロイルカテキン類の抗酸化活性の促進方法 (2008)
  • Inventor(s): 立花宏文
  • Industrial Property Rights Holder: 国立大学法人九州大学
  • Industrial Property Number: 特許・特願2008-332205
  • Filing Date: 2008-12-26