Regulation of membrane traffic and ubiquitination by small GTPases

2008 Fiscal Year Final Research Report

• Project/Area Number: 18209003
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Kyoto University
• Principal Investigator: NAKAYAMA Kazuhisa Kyoto University, 薬学研究科, 教授 (40192679)
• Project Period (FY): 2006 – 2008
• Keywords: 小胞輸送 / メンブレントラフィック / 低分子量GTPase / ユビキチン / 細胞質分裂

Research Abstract

Ubiquitination serves as a signal for protein degradation by the proteasome as well as regulates various aspects of membrane trafficking. Furthermore, ubiquitination has been revealed to play a crucial role in cytokinesis. On the other hand, membrane trafficking is regulated by the Arf and Rab families of small GTPases. In this study, I have studied on regulation of membrane trafficking and cytokinesis by proteins that not only bind to the small GTPases but also undergo ubiquitination and/or ubiquitin binding, and identified a part of the regulatory mechanisms.

Research Products

• [Journal Article] Three homologous ArfGAPs participate in coat protein I-mediated transport. (2009)
  • Author(s): Saitoh, A., Shin, H.-W., Yamada, A., Waguri, S., Nakayama, K.
  • Journal Title: J. Biol. Chem. 284 Pages: 13948-13957
  • Peer Reviewed
• [Journal Article] Differential effects of depletion of ARL1 and ARFRP1 on membrane trafficking between the trans-Golgi network and endosomes. (2009)
  • Author(s): Nishimoto-Morita, K., Shin, H.-W., Mitsuhashi, H., Kitamura, M., Zhang, Q., Johannes, L., Nakayama, K.
  • Journal Title: J. Biol. Chem. 284 Pages: 10583-10592
  • Peer Reviewed
• [Journal Article] The retroendocytosis pathway of ABCA1/apoA-I contributes to HDL formation. (2009)
  • Author(s): Azuma, Y., Takada, M., Shin, H.-W., Kioka, N., Nakayama, K., Ueda, K.
  • Journal Title: Genes Cells 14 Pages: 191-204
  • Peer Reviewed
• [Journal Article] Redundant roles of BIG2 and BIG1, guanine-nucleotide exchange factors for ARFs, in membrane traffic between the TGN and endosomes. (2008)
  • Author(s): Ishizaki, R., Shin, H.-W., Mitsuhashi, H., Nakayama, K.
  • Journal Title: Mol. Biol. Cell 19 Pages: 2650-2660
  • Peer Reviewed
• [Journal Article] Recruitment of Tom1L1/Srcasm to endosomes and the midbody by Tsg101. (2008)
  • Author(s): Yanagida-Ishizaki, Y., Takei, T., Ishizaki, R., Imakagura, H., Takahashi, S., Shin, H.-W., Katoh, Y., Nakayama, K.
  • Journal Title: Cell Struct. Funct. 33 Pages: 91-100
  • Peer Reviewed
• [Journal Article] Functional involvement of TMF/ARA160 in Rab6-dependent retrograde membrane traffic (2007)
  • Author(s): Yamane, J., Kubo, A., Nakayama, K., Yuba-Kubo, A. Katsuno, T., Tsukita, S., Tsukita, S.
  • Journal Title: Exp. Cell Res. 313 Pages: 3472-3485
  • Peer Reviewed
• [Journal Article] Molecular basis for autoregulatory interaction between GAE domain and hinge region of GGA1. (2007)
  • Author(s): Inoue, M., Shiba, T., Ihara, K., Yamada, Y., Hirano, S., Kamikubo, H., Kataoka, M., Kawasaki, M., Kato, R., Nakayama, K., Wakatsuki, S.
  • Journal Title: Traffic 8 Pages: 904-913
  • Peer Reviewed
• [Journal Article] Activation of histidine decarboxylase through post-translational cleavage by caspase-9 in a mouse mastocytoma P-815. (2007)
  • Author(s): Furuta, K., Nakayama, K., Sugimoto, Y., Ichikawa, A., Tanaka, S.
  • Journal Title: J. Biol. Chem. 282 Pages: 13438-13446
  • Peer Reviewed
• [Journal Article] Structural basis for Rab11-dependent membrane recruitment of FIP3/Arfophilin-1. (2006)
  • Author(s): Shiba, T., Koga, H., Shin, H.-W., Kawasaki, M., Kato, R., Nakayama, K., Wakatsuki, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA 103 Pages: 15416-15421
  • Peer Reviewed
• [Journal Article] Monoubiquitylation of GGA3 by hVPS18 regulates its ubiquitin-binding ability. (2006)
  • Author(s): Yogosawa, S., Kawasaki, M., Wakatsuki, S., Kominami, E., Shiba, Y., Nakayama, K., Kohsaka, S., Akazawa, C.
  • Journal Title: Biochem. Biophys. Res. Commun. 350 Pages: 82-90
  • Peer Reviewed
• [Journal Article] Membrane orientation of the precursor 74-kDa form of L-histidine decarboxylase. (2006)
  • Author(s): Furuta, K., Ichikawa, A., Nakayama, K., Tanaka, S.
  • Journal Title: Inflamm. Res. 55 Pages: 185-191
  • Peer Reviewed
• [Journal Article] AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors. (2006)
  • Author(s): Ishizaki, R., Shin, H.-W., Iguchi-Ariga, S.M.M., Ariga, H., Nakayama, K.
  • Journal Title: Genes Cells 11 Pages: 949-959
  • Peer Reviewed
• [Journal Article] Recruitment of clathrin onto endosomes by the Tom1-Tollip complex. (2006)
  • Author(s): Katoh, Y., Imakagura, H., Futatsumori, M., Nakayama, K.
  • Journal Title: Biochem. Biophys. Res. Commun. 341 Pages: 143-149
  • Peer Reviewed
• [Presentation] CRESCent:紡錘体極中心体のまわりに形成されるリサイクリングエンドソームのクラスター (2010)
  • Author(s): 中山和久
  • Organizer: 第115回日本解剖学会全国学術集会シンポジウム
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-28
• [Presentation] CRESCent: Cluster of Recycling Endosomes Transiently Formed around Spindle Pole Centrosome. (2009)
  • Author(s): Takatsu, H., Ueda, T., Waguri, S., Takahashi, S., Kondo, Y., Shin, H. -W., Nakayama, K.
  • Organizer: 49th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: San Diego
  • Year and Date: 2009-12-07
• [Presentation] RabファミリーとArfファミリーの低分子量GTPaseによる細胞質分裂の調節 (2009)
  • Author(s): 中山和久, 高橋千絵, 武井朋美, 上田智子, 高津宏之, 申惠媛
  • Organizer: 第82回日本生化学会大会シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] 分裂間期および細胞分裂時のFIP3/FIP4の局在化におけるRab11およびArf6の役割 (2009)
  • Author(s): 高橋千絵, 武井朋美, 古賀裕士, 申惠媛, 中山和久
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] 細胞質分裂時のArf6およびEFA6(Arf6 GEF)の局在変化 (2009)
  • Author(s): 上田智子, 武井朋美, 申惠媛, 阪上洋行, 中山和久
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] 細胞質分裂における低分子量GTPase Arf6の機能の解析 (2009)
  • Author(s): 武井朋美, 高橋千絵, 申惠媛, 中山和久
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-02
• [Presentation] 細胞分裂時におけるリサイクリングエンドソームの動態 (2009)
  • Author(s): 高津宏之, 上田智子, 近藤由美香, 申惠媛, 中山和久
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-02
• [Presentation] ArfGAP1, 2, 3はCOPI被覆小胞を介する輸送経路で働く (2009)
  • Author(s): 齋藤明奈, 申惠媛, 山田茜, 和栗聡, 中山和久
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-02
• [Presentation] メンブレントラフィックにおけるArfaptin/PORの機能研究 (2009)
  • Author(s): 満智秋, 申恵媛, 中山和久
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-02
• [Presentation] リソソーム分解経路における低分子量GTPase ARF3の機能 (2009)
  • Author(s): 申惠媛, 近藤由美香, 中山和久
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-02
• [Presentation] タンパク質の細胞膜への輸送とエンドサイトーシスの調節 (2009)
  • Author(s): 中山和久
  • Organizer: 第82回日本薬理学会年会シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2009-03-18
• [Presentation] Arfのグアニンヌクレオチド交換因子BIG1とBIG2のトランスゴルジ網への局在機構の解析 (2008)
  • Author(s): 高島皓平, 申惠媛, 中山和久
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 細胞内小胞輸送における低分子量GTPase Rab14の役割 (2008)
  • Author(s): 山本昆明, 申惠媛, 中山和久
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] 低分子量GTPaseによるリサイクリングエンドソーム機能と細胞質分裂の調節 (2008)
  • Author(s): 中山和久, 高橋千絵, 武井朋美, 高津宏之, 申惠媛
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] ARL1 and ARFRP1 independently regulate membrane trafficking‘to' and‘from' the TGN (2008)
  • Author(s): Shin, H. -W., Nishimoto, K., Nakayama, K.
  • Organizer: The 2008 Golgi meeting
  • Place of Presentation: Pavia, Italy
  • Year and Date: 2008-09-07
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/physchem/