Phylogeographical study of dipterocarp species and development of molecular database for identification of its origin.

2009 Fiscal Year Final Research Report

• Project/Area Number: 18255010
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: 林学・森林工学
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: TSUMURA Yoshihiko Forestry and Forest Products Research Institute, 森林遺伝研究領域, 室長 (20353774)
• Co-Investigator(Kenkyū-buntansha): UENO Saneyoshi 森林総合研究所, 森林遺伝研究領域, 主任研究員 (40414479) ; TANI Naoki 国際農林水産業研究センター, 林業領域, 主任研究員 (90343798)
• Co-Investigator(Renkei-kenkyūsha): TOMARU Nobuhiro 名古屋大学, 生命農学研究科, 教授 (50241774) ; ALFRED Szmidt E. 九州大学, 理学部, 準教授 (70325497) ; IWATA Hiroyoshi 農業・生物特定産業技術研究機構, 中央農研, 主任研究院 (00355489)
• Project Period (FY): 2006 – 2009
• Keywords: フタバガキ科 / 遺伝構造 / 産地識別 / 系統地理 / 遺伝的多様性

Research Abstract

We have collected 920 individuals of 28 populations in Shorea leprosula throughout its distribution area such as Malaysia and Indonesia. In S.parvifolia, 404 individuals of 13 populations were collected from Malaysia and Indonesia. DNA markers for population samples were developed which are 40 loci of EST-SSR markers derived from cDNA sequence data. Intraspecific chloroplast DNA polymorphisms were screened of 17 regions of intergenic regions, and found 5 regions (trnD-trnT、petB intron、petL-psbE、psbJ-petA、rps16-trnK) were polymorphic in S.leprosula and 4 regions petL-psbE、psbJ-petA、rpl32-trnL、trnQ-rps16) in S.parvifolia. The result of nuclear DNA markers such as 34 loci of EST-SSRs showed clear genetic differentiation between Borneo and the other populations, although the Fst=0.0671 was low. The result of chloroplast DNA (cpDNA) polymorphism showed much clear genetic differentiation between Borneo and the other populations, and 21 cpDNA haplotypes was found and the coefficient of genetic differentiation, Fst value was quite high, 0.5851. These results suggest that there were two different origins of current tropical forest in Southeast Asia. According to cpDNA results, region identification was possible but the identification of each population would be difficult by only DNA data.

Research Products

• [Journal Article] Characterization of 12 microsatellite loci for important tropical tree species Shorea maxwelliana and S. laevis (Dipterocarpaceae) (2010)
  • Author(s): Masuda, S., N. Tani, M. Ohtani, Y. Tsumura
  • Journal Title: Conservation Genetics Resources (in press)
  • Peer Reviewed
• [Journal Article] Molecular database for classifying Shorea species (Dipterocarp aceae) and techniques for checking the legitimacy of timber and wood products. (2010)
  • Author(s): Tsumura, Y., T. Kado, K. Yoshida, H. Abe, M. Ohtani, Y. Taguchi, Y. Fukue, N. Tani, S. Ueno, K. Yoshimura, K. Kamiya, K. Harada, Y. Takeuchi, B. Diway, R. Finkeldey, M. Na'iem, S. Indrioko, K.K.S. Ng, N. Muhammad, S.L. Lee
  • Journal Title: ournal of Plant Research (in press)
  • Peer Reviewed
• [Journal Article] EST-SSRsisolated from Shorea leprosula and their transferability to 36 species within the Dipterocarpaceae. (2009)
  • Author(s): Ng, K.K.S., S.L. Lee, Y. Tsumura, S. Ueno, C.H. Ng, C.T. Lee
  • Journal Title: Molecular Ecology Resources 9 Pages: 393-398
  • Peer Reviewed
• [Journal Article] Paternity analysis-based inference of pollen dispersal patterns, male fecundity variation and influence of flowering tree densities in two dipteroc arp species. (2009)
  • Author(s): Tani, N., Tsumura, Y., Kado, T., Taguchi, Y., Lee, S.L., Muhammad, N., Ng. K.K.S., Numata, S., Nishimura, S., Konuma, A., Okuda, T.
  • Journal Title: Annals of Botany 104 Pages: 1421-1434
  • Peer Reviewed
• [Journal Article] Density dependent selfing and its effects on seed performance in a tropicalcanopy tree species, Shorea acuminata (Dipterocarpaceae). (2008)
  • Author(s): Naito Y., M. Kanzaki, H. Iwata, K. Obayashi, S.L. Lee, N. Muhammad, T. Okuda, Y. Tsumura
  • Journal Title: Forest Ecology and Management 256 Pages: 375-383
  • Peer Reviewed
• [Journal Article] Size-related flowering and fecundity in the tropical canopy tree species (2007)
  • Author(s): Naito, Y., M. Kanzaki, S. Numata, K. Obayashi, A. Konuma, S. Nishimura, S. Ohta, Y. Tsumura, T. Okuda, Lee S.L., N. Muhammad
  • Journal Title: Shorea acuminate (Dipterocarpac eae) during two consecutive general flowerings 121 Pages: 33-42
  • Peer Reviewed
• [Journal Article] Effects of flowering tree-density on mat ing system and gene flow in Shorea lepr osula (Dipterocarpaceae) in Peninsular Malaysia. (2007)
  • Author(s): Fukue, Y., T. Kado, S.L. Lee, K.K.S. Ng, N. Muhammad, Y. Tsumura
  • Journal Title: Journal of Plant Research 120 Pages: 413-420
  • Peer Reviewed
• [Presentation] マレー半島におけるフタバガキ科樹木Shorea maxwellianaの遺伝子流動の年次変動 (2010)
  • Author(s): 増田真佑, 谷尚樹, 大谷雅人, 上野真義, Norwati Muhammad, Soon Leong Lee, 津村義彦
  • Organizer: 日本生態学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-03-17
• [Presentation] Molecular database for classifying Shorea species (Dipterocarpaceae) and searching the origin of timber in some Shorea species. (2009)
  • Author(s): Tsumura Y., T. Kado, K. Yoshida, H. Abe, M. Ohtani, Y. Taguchi, Y. Fukue, N. Tani, S. Ueno, K. Yoshimura, K. Kamiya, K. Harada, Y. Takeuchi, B. Diway, R. Finkeldey, M. Na' iem, S. Indrioko, K.K.S. Ng, N. Muhammad, S.L. Lee
  • Organizer: International Forest Genetic Resources Symposium-Conservation and sustainable utilization towards climate change mitigation and adaptation
  • Place of Presentation: Kuala Lumpur
  • Year and Date: 2009-10-06
• [Presentation] 東南アジア熱帯雨林における花粉散布研究 (2009)
  • Author(s): 谷尚樹、角友之、深澤圭太、田口由里子、LeeS。L.、NgK.K.S.、M. Norwati、R. b. Azizi、沼田真也、西村千、小沼明弘、奥田敏統、新山馨、津村義彦
  • Organizer: 日本森林学会
  • Place of Presentation: 京都大学
  • Year and Date: 2009-03-27
• [Presentation] マイクロサテライトマーカーを用いたフタバガキ科Shorea maxwellianaの遺伝子流動 (2009)
  • Author(s): 増田真佑・谷尚樹・上野真義・Norwati Muhammad・Soon Leong Lee・津村義彦
  • Organizer: 日本森林学会
  • Place of Presentation: 京都大学
  • Year and Date: 2009-03-27
• [Presentation] 違法伐採抑止のためのフタバガキ科Shorea属種識別DNAデータベースの構築 (2007)
  • Author(s): 津村義彦, 角友之, 福江陽子, 谷直樹, 柴田正志, 上谷浩一, 原田光, 竹内やよい, Diway Bibian, Lee Soon Leong, Norwati Muhammad, Finkeldey Reiner
  • Organizer: 日本森林学会
  • Place of Presentation: 九州大学
  • Year and Date: 20070402-20070404