2007 Fiscal Year Final Research Report Summary
Development of photoacoustic imaging with near-infrared pulse laser to improve depth resolution
| Project/Area Number |
18300153
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TAKAMATSU Tetsuro Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Professor (40154900)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAOKA Yoshiisa Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Assistant Professor (80405274)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Photoacoutic imaging / Bioimaging / Nonlinear optics / Multiphoton absorption / Infrared lights / Ultrafast light pulses / Medical and biological engineering / Vascular imaging |
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| Research Abstract |
Commercial imaging systems, such as computed tomography (CT) and magnetic resonance imaging (MRI), are frequently used powerful tools for observing structures deep within the human body. However, they cannot precisely visualized several-tens micrometer-sized structures for lack of spatial resolution. In this research, we proposed a multiphoton excitation-assisted photoacoustic tomography (MEAPAT) as a means of improving depth resolution. Since the multiphoton absorption occurs at only the focus point and the employed infrared pulses deeply penetrate living tissues, it enables us to extract characteristic features of structures embedded in the living tissue. When nanosecond pulses from a 1064-nm Nd:YAG laser were focused on Rhodamine B solution (absorption peak: 540 nm), the peak intensity of the generated photoacoustic signal detected by a 10 MHz transducer was proportional to the square of the input pulse energy. This result shows that the photoacoustic signals can be induced by the two-photon absorption of infrared nanosecond pulse laser and also can be detected by a commercial low-frequency MHz transducer. This means that MEAPAT overcomes the depth limitation of existing one-photon photoacoustic tomography caused by the need of a high-frequency transducer to improve depth resolution. Furthermore, in order to evaluate the depth resolution of MEAPAT, we compared the image of MEAPAT with that of one-photon photoacoustic imaging of a vascular phantom in a water bath. As a result, we found that the depth resolution of MEAPAT (1064 nm) is greater than that of one-photon photoacoustic imaging (532 nm).We conclude that evolving multiphoton-photoacoustic imaging technology renders feasible the investigation of biomedical phenomena at the deep layer in living tissue.
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[Journal Article] Imaging of anticancer agent distribution by a slit-scanning Raman microscope2008
Author(s)
Harada, Y., Ota, T., Dai, P., Yamaoka, Y., Hamada, K., Fujita, K., Takamatsu, T
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Journal Title
Proceedings of SPIE 6853
Pages: 685308-1-685308-5
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mucosal layer as major source of green autofluorescence in the colon under excitation by blue light2008
Author(s)
Nakano, K., Harada, Y., Yamaoka, Y., Miyawaki, K., Wakabayashi, N., Mitsufuji, S., Imaizumi, K., Takaoka, H., Nakaoka, M., Takamatsu, T
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Journal Title
Proceedings of SPIE 6853
Pages: 68531H-1-68531H-6
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Generation of reentrant arrhythmias by dominant-nagative inhibition of connexin43 in rat cultured myocyte monolayers2008
Author(s)
Nakagami, T., Tanaka, H., Dai, P., Tanabe, T., Math, H., Fujiwara, K., Lin, SF., Matsubara, H., Takamatsu, T
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Journal Title
Cardiovascular Research in press
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Location-dependent photogeneration of calcium waves in HeLa cells2006
Author(s)
Iwanaga, S., Kaneko, T., Fujita, K., Smith, N., Nakamura, O., Takamatsu, T., Kawata, S
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Journal Title
Cell Biochemistry and Biophysics 45
Pages: 167-176
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Mucosal layer as major source of green autofluorescence in the colon under excitation by blue light2008
Author(s)
Nakano, K., Harada, Y., Yamaoka, Y., Miyawaki, K., Wakabayashi, N., Mitsufuji, S., Imaizumi, K., Takaoka, H., Nakaoka, M., Takamatsu, T
Organizer
Photonics West 2008
Place of Presentation
San Jose
Year and Date
2008-01-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Imaging of anticancer agent distribution by a slit-scanning Raman microscope2008
Author(s)
Harada, Y., Ota, T., Dai, P., Yamaoka, Y., Hamada, K., Fujita, K., Takamatsu, T
Organizer
Photonics West 2008
Place of Presentation
San Jose
Year and Date
2008-01-19
Description
「研究成果報告書概要(欧文)」より
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