Etiological mechanism of lifestyle-related diseases caused by disordered daily rhythms

2009 Fiscal Year Final Research Report

• Project/Area Number: 18300228
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied health science
• Research Institution: Chiba University
• Principal Investigator: TAKIGUCHI Masaki Chiba University, 大学院・医学研究院, 教授 (40179578)
• Co-Investigator(Kenkyū-buntansha): HIWASA Takaki 千葉大学, 大学院・医学研究院, 准教授 (30260251) ; TAMURA Yutaka 千葉大学, 大学院・医学研究院, 准教授 (50263174) ; IWASE Katuro 千葉大学, 大学院・医学研究院, 講師 (80322030) ; MATUMOTO Eriko 千葉大学, 大学院・医学研究院, 技術職員 (20422256)
• Project Period (FY): 2006 – 2009
• Keywords: 生活習慣病

Research Abstract

Expression of the gene for sterol regulatory element-binding protein-1 (SREBP-1), a transcription factor pivotal in lipid biosynthesis, achieved a peak in the dark feeding period of the daily light/dark cycle in the liver of mice fed with a standard diet, and a peak in the light fasting period with high-carbohydrate, high-fat, and high-protein diets, underlining the importance of nutrients in shaping the daily rhythm. Expression of genes for the ornithine cycle, an enzyme system for ammonia detoxification, achieved a peak in the feeding period with a high-protein diet, and a peak in the late fasting to early feeding periods with a low-protein (high-carbohydrate) diet, suggesting activation of the expression both in the feeding and fasting periods because of requirement for detoxifying ammonia derived form amino acid degradation.

Research Products

• [Journal Article] Activation of NFAT signal by p53-K120R mutant (2009)
  • Author(s): Shinmen, N., Koshida, T., Kumazawa, T., Sato, K., Shimada, H., Matsutani, T., Iwadate, Y., Takiguchi, M., Hiwasa, T.
  • Journal Title: FEBS Lett. 583 Pages: 1916-1922
  • Peer Reviewed
• [Journal Article] Insight into estrogenicity of phytoestrogens using in silico simulation (2009)
  • Author(s): Sugiyama, H., Kumamoto, T., Suganami, A., Nakanishi, W., Sowa, Y., Takiguchi, M., Ishikawa, T., Tamura, Y.
  • Journal Title: Biochem.Biophys.Res.Commun. 379 Pages: 139-144
  • Peer Reviewed
• [Journal Article] Hepatoblast-like cells enriched from mouse embryonic stem cells in medium without glucose, pyruvate, arginine, and tyrosine (2008)
  • Author(s): Tomizawa, M., Toyama, Y., Ito, C., Toshimori, K., Iwase, K., Takiguchi, M., Saisho, H., Yokosuka, O.
  • Journal Title: Cell Tissue Res. 333 Pages: 17-27
  • Peer Reviewed
• [Journal Article] Expression of the WT1 gene-KTS domain isoforms suppresses the invasive ability of human lung squamous cell carcinoma cells (2008)
  • Author(s): Moriya, S., Takiguchi, M., Seki, N.
  • Journal Title: Int.J.Oncol. 32 Pages: 349-356
  • Peer Reviewed
• [Journal Article] Multifactorial regulation of daily rhythms in expression of the metabolically responsive gene Spot14 in the mouse liver (2007)
  • Author(s): Ishihara, A., Matsumoto, E., Horikawa, K., Kudo, T., Sakao, E., Nemoto, A., Iwase, K., Sugiyama, H., Tamura, Y., Shibata, S., Takiguchi, M.
  • Journal Title: J.Biol.Rhythms 22 Pages: 324-334
  • Peer Reviewed
• [Journal Article] Altered gene expression in the subdivisions of the amygdala of Fyn-deficient mice as revealed by laser capture microdissection and mKIAA cDNA array analysis (2006)
  • Author(s): Kai, N., Iwase, K., Imai, K., Nakahira, E., Soma, M., Ohtsuka, S., Yagi, T., Kobayashi, K., Koga, H., Takiguchi, M., Yuasa, S.
  • Journal Title: Brain Res. 1073-1074 Pages: 60-70
  • Peer Reviewed
• [Journal Article] Up-regulation of genes for oxidative phosphorylation and protein turnover in diabetic mouse retina (2006)
  • Author(s): Adachi-Uehara, N., Kato, M., Nimura, Y., Seki, N., Ishihara, A., Matsumoto, E., Iwase, K., Ohtsuka, S., Kodama, H., Mizota, A., Yamamoto, S., Adachi-Usami, E., Takiguchi, M.
  • Journal Title: Exp.Eye Res. 83 Pages: 849-857
  • Peer Reviewed
• [Journal Article] Enhancement of chemosensitivity toward peplomycin by calpastatin-stabilized NF-kappaB p65 in esophageal carcinoma cells : possible involvement of Fas/Fas-L synergism (2006)
  • Author(s): Liu, T. L., Shimada, H., Ochiai, T., Shiratori, T., Lin, S. E., Kitagawa, M., Harigaya, K., Maki, M., Oka, M., Abe, T., Takiguchi, M., Hiwasa, T.
  • Journal Title: Apoptosis 11 Pages: 1025-1037
  • Peer Reviewed
• [Journal Article] Activation of genes for growth factor and cytokine pathways late in chondrogenic differentiation of ATDC5 cells (2006)
  • Author(s): Osawa, A., Kato, M., Matsumoto, E., Iwase, K., Sugimoto, T., Matsui, T., Ishikura, H., Sugano, S., Kurosawa, H., Takiguchi, M., Seki, N.
  • Journal Title: Genomics 88 Pages: 52-64
  • Peer Reviewed
• [Journal Article] Relationship between pancreatic secretory trypsin inhibitor and early recurrence of intrahepatic cholangiocarcinoma following surgical resection (2006)
  • Author(s): Tonouchi, A., Ohtsuka, M., Ito, H., Kimura, F., Shimizu, H., Kato, M., Nimura, Y., Iwase, K., Hiwasa, T., Seki, N., Takiguchi, M., Miyazaki, M.
  • Journal Title: Am.J.Gastroenterol. 101 Pages: 1601-1610
  • Peer Reviewed
• [Presentation] Ca^<2+>およびcAMPによるセクレトグラニンII遺伝子の協調的発現制御の解析 (2009)
  • Author(s): 岩瀬克郎
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] マウス肝臓におけるオルニチンサイクル酵素遺伝子の日周発現リズムの調節機構 (2009)
  • Author(s): 松本絵里子
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] マウス肝臓におけるSREBP-1c遺伝子発現の概日時計と栄養要因による制御 (2008)
  • Author(s): 松本絵里子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] マウス肝臓におけるオルチニンサイクル酵素遺伝子発現の日周リズム (2008)
  • Author(s): 玉井咲貴
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] マウス肝臓における代謝応答性 Spot14 遺伝子の発現の日周リズムに見られる多因子制御 (2007)
  • Author(s): 松本絵里子
  • Organizer: 第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] マウス視交叉上核において光刺激によって制御される遺伝子の解析 (2007)
  • Author(s): 根本あや子
  • Organizer: 第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] cAMP response element binding protein (CREB) Ser133 の脱リン酸化機構の解析 (2007)
  • Author(s): 吉村俊太朗
  • Organizer: 第80回日本生化学会大会・第30回日本分子生物学会年会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-10
• [Presentation] Daily rhythm of SREBP gene expression in the mouse liver (2006)
  • Author(s): Matsumoto, E.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-21
• [Presentation] Regulation of secretoneurin gene expression by neurotransmitter (2006)
  • Author(s): Iwase, K.
  • Organizer: 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: Kyoto
  • Year and Date: 2006-06-20
• [Patent(Industrial Property Rights)] 微量mRNA及びcDNAの増幅方法 (2006)
  • Inventor(s): 瀧口正樹
  • Industrial Property Rights Holder: 独立行政法人科学技術振興機構
  • Industrial Property Number: 特許特許第3853161号
  • Acquisition Date: 2006-09-15