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2008 Fiscal Year Final Research Report

Environmental Assessment Based on Synoptic Molecular Taxonomy of Soil Bacterial DNA

Research Project

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Project/Area Number 18310009
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental dynamic analysis
Research InstitutionYokohama National University

Principal Investigator

ITOH Kiminori  Yokohama National University, 大学院・工学研究院, 教授 (40114376)

Co-Investigator(Kenkyū-buntansha) AMEMIYA Takashi  横浜国立大学, 大学院・工学研究院, 准教授 (60344149)
Project Period (FY) 2006 – 2008
Keywords環境計測
Research Abstract

環境中で重要な機能を担うにも関わらず、その詳細が不明な土壌細菌群集に関して、実験的・理論的な解析を行った。実験的には、DNA を高い分離能の検出できる二次元電気泳動法と、異なるDNA の集合を全体的に見るための総観的分子分類学に基づいて、新しい評価法を開発した。二次元電気泳動では、それぞれの細菌種を、1 つ1 つのスポットとして得ることができるため、全体の見通しが良い。理論的には、多様性が出現する機構を、「食う・食われる」の関係を表す食物網から検討すると共に、生態系中の非線形な相互作用による動態の解析を行い、典型的な湖汚染であるアオコ(シアノバクテリアの集団)の季節変動や日内変動などの挙動を明らかにした

  • Research Products

    (34 results)

All 2009 2008 2007 2006

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (16 results) Book (3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Identification and Validation of suitable Reference Genes for Quantitative Expression of xylA and xylE Genes in Pseudomonas putida mt-22009

    • Author(s)
      Qing Chang, Takashi Amemiya, Jianbo Liu, Xianjing Xu, and Kiminori Itoh
    • Journal Title

      Journal of Bioscience and Bioengineering (印刷中)

    • Peer Reviewed
  • [Journal Article] Noise-triggered regime shifts in a simple aquatic model2009

    • Author(s)
      H. Serizawa, T. Amemiya, K. Itoh
    • Journal Title

      Ecological Complexity (印刷中)

    • Peer Reviewed
  • [Journal Article] Patchiness and bistability in the comprehensive cyanobacterial model(CCM)2009

    • Author(s)
      Hiroshi Serizawa ; Takashi Amemiya ; Kiminori Itoh
    • Journal Title

      Ecological Modelling 220

      Pages: 764-773

    • Peer Reviewed
  • [Journal Article] The top-down mechanism for body mass -abundance scaling2008

    • Author(s)
      A. G. Rossberg, R. Ishii, T. Amemiya, and K. Itoh
    • Journal Title

      Ecology 89(2)

      Pages: 567-580

    • Peer Reviewed
  • [Journal Article] Two-Dimensional DNA Gel Electrophoresis Mapping2008

    • Author(s)
      Guohua Liu, Takashi Amemiya, and Kiminori Itoh
    • Journal Title

      a Novel Approach to Diversity Analysis of Bacterial Communities in Environmental Soil J. Bioscience and Bioengineering 105

      Pages: 127-133

    • Peer Reviewed
  • [Journal Article] Mathematical modeling of colony formation in algal blooms2008

    • Author(s)
      H. Serizawa, T. Amemiya, T. Enomoto, A. G. Rossberg, and K. Itoh
    • Journal Title

      phenotypic plasticity in cyanobacteria, Ecological Research 23

      Pages: 841-850

    • Peer Reviewed
  • [Journal Article] Computer simulations of seasonal outbreak and diurnal vertical migration of cyanobacteria2008

    • Author(s)
      Hiroshi Serizawa, Takashi Amemiya, Axel G. Rossberg, and Kiminori Itoh
    • Journal Title

      Limnology 9

      Pages: 185-194

    • Peer Reviewed
  • [Journal Article] Spatial distribution of phytoplankton in a mathematical model2008

    • Author(s)
      Hiroshi Serizawa, Takashi Amemiya, and Kiminori Itoh
    • Journal Title

      Journal of Biosciences 33

      Pages: 391-403

    • Peer Reviewed
  • [Journal Article] Stability and dynamical behavior in a lake-model and implications for regime shifts in real lakes ecological modeling2007

    • Author(s)
      T. Amemiya, T. Enomoto, A. G. Rossberg, T. Yamamoto, Y. Inamori, and K. Itoh
    • Journal Title

      Ecological Modelling 206

      Pages: 54-62

    • Peer Reviewed
  • [Journal Article] Some properties of the speciation model for foodweb structure Mechanisms for degree distributions and intervality2006

    • Author(s)
      A.G. Rossberg, H. Matsuda, T. Amemiya, K. Itoh
    • Journal Title

      Journal of Theoretical Biology 238

      Pages: 401-415

    • Peer Reviewed
  • [Journal Article] Food Webs : Experts Consuming Families of Experts2006

    • Author(s)
      Axel G. Rossberg ; Hiroyuki Matsuda ; Takashi Amemiya ; Kiminori Itoh
    • Journal Title

      Food webs : Experts consuming families of experts, J. Theoretical Biology 241(3)

      Pages: 552-563

    • Peer Reviewed
  • [Journal Article] Estimating trophic link density from quantitative but incomplete diet data2006

    • Author(s)
      Axel G. Rossberg, K Yanagi, Takashi Amemiya, Kiminori Itoh
    • Journal Title

      Journal of Theoretical Biology 243

      Pages: 261-272

    • Peer Reviewed
  • [Journal Article] Development of a two-dimensional electrophoresis method to study soil bacterial diversity2006

    • Author(s)
      Takumi Isshi, Rajendran Narasimmalu, Takashi Amemiya and Kiminori Itoh
    • Journal Title

      Soil Science and Plant Nutrition 52

      Pages: 601-609

    • Peer Reviewed
  • [Presentation] A Rapid Analysis for Environmental Bacterial Community Structure based on Selective Primer Pairs-Two dimensional DNA Gel Electrophoresis Mapping2009

    • Author(s)
      G.-H. Liu, T. Amemiya, K. Itoh
    • Organizer
      FEMS 2009-3rd Congress of European Microbiologists
    • Place of Presentation
      Gothernborg(Sweden)(発表予定)
    • Year and Date
      20090628-0702
  • [Presentation] キチン培地による殺藻細菌の単離-スキムミルク培地との比較-2009

    • Author(s)
      柴田賢一, 雨宮隆, 伊藤公紀
    • Organizer
      H21 年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2009-03-29
  • [Presentation] Understanding nonlinearity in ecosystems for benefitting sustainable ecosystem services2009

    • Author(s)
      Amemiya T
    • Organizer
      International Conference on Sustainability Science 2009 (ICSS2009)
    • Place of Presentation
      The University of Tokyo (Tokyo)
    • Year and Date
      2009-02-05
  • [Presentation] 湖沼の富栄養化によるアオコ(藍藻類)のパターン形成2009

    • Author(s)
      雨宮隆, 芹沢浩, 伊藤公紀
    • Organizer
      第18回非線形反応と協同現象研究会
    • Place of Presentation
      同志社大学(京都)
    • Year and Date
      2009-01-10
  • [Presentation] Relative Quantification of Gene Expression During Biodegradation to Select Reliable Reference Genes2008

    • Author(s)
      Qing Chang, Takashi Amemiya, Jianbo Liu and Kiminori Itoh
    • Organizer
      24^<th> Annual International Conference on Soils, Sediments and Water
    • Place of Presentation
      University of Massachusetts in Amherst, (USA)
    • Year and Date
      20081020-23
  • [Presentation] Influence of Organic Matter Sources on Bacterial Biomass in The Surface Sediments of Coastal Lagoon Environments Stable Isotope and Quantitative PCR Analyses2008

    • Author(s)
      S. Tsuboi, Y. Urushigawa, K. Seto, Takashi Amemiya, Kiminori Itoh
    • Organizer
      The 12^<th> International Symposium on Microbial Ecology、Cairns
    • Place of Presentation
      Austraria
    • Year and Date
      20080817-22
  • [Presentation] 細菌DNA の二次元電気泳動法の開発とその生態リスク評価への応用2008

    • Author(s)
      劉国華, 原田智幸, 雨宮隆, 伊藤公紀
    • Organizer
      日本微生物生態学会第24 回大会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2008-11-26
  • [Presentation] 顕微FT-IR によるアオコ(Microcystis 属)の生体内分子組成の解析2008

    • Author(s)
      安海日, 柴田賢一, 雨宮隆, 伊藤公紀
    • Organizer
      日本微生物生態学会第24 回大会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2008-11-26
  • [Presentation] 実下水処理場におけるリン除去への影響因子 : 微生物群集変化の長期モニタリングに着目して2008

    • Author(s)
      砂田尚孝, 雨宮隆, 伊藤公紀
    • Organizer
      日本微生物生態学会第24回大会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2008-11-26
  • [Presentation] Desulfitobacterium sp. PCE1株によるテトラクロロエチレン分解の分子生物学的研究2008

    • Author(s)
      坪井隼, Narasimmalu Rajendran, 雨宮隆, 伊藤公紀
    • Organizer
      日本微生物生態学会第24回大会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2008-11-26
  • [Presentation] 大腸菌を用いたバイオアドソープションによる重金属除去2008

    • Author(s)
      濱島隆浩, 雨宮隆, 伊藤公紀
    • Organizer
      日本微生物生態学会第24回大会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2008-11-26
  • [Presentation] Activities of algicidal bacteria and their influences on microbial communities2008

    • Author(s)
      Shibata K, Amemiya T, Itoh K
    • Organizer
      JST Presto Symposium on Mathematical Sciences towards Environmental Problems
    • Place of Presentation
      Hokkaido University (Sapporo)
    • Year and Date
      2008-06-11
  • [Presentation] 二次元電気泳動法による環境細菌の多様性解析2007

    • Author(s)
      大木智行・原田知幸・雨宮隆・伊藤公紀
    • Organizer
      日本化学会第87春季年会
    • Place of Presentation
      関西大学(大阪)
    • Year and Date
      20070325-28
  • [Presentation] 藍藻類Microcyst is sp.の細胞内分子組成と生理生態の関連性2007

    • Author(s)
      渡辺悠介・榎本隆寿・雨宮隆・伊藤公紀
    • Organizer
      日本化学会第87春季年会
    • Place of Presentation
      関西大学(大阪)
    • Year and Date
      20070325-28
  • [Presentation] リアルタイムRT-PCR 法を用いたToluene 誘導下におけるtodC1遺伝子発現の相対定量2007

    • Author(s)
      劉建波, Narasimmalu Rajendran, 伊藤公紀, 雨宮隆
    • Organizer
      日本化学会第87春季年会
    • Place of Presentation
      関西大学(大阪)
    • Year and Date
      20070325-28
  • [Presentation] Two-Dimensional DNA Electrophoresis Mapping : Comparison of Dominant Bacteria in Trichloroethylene(TCE)-Contaminated Sites2007

    • Author(s)
      G.H. Liu, T. Amemiya, K. Itoh
    • Organizer
      日本化学会第87春季年会
    • Place of Presentation
      関西大学(大阪)
    • Year and Date
      20070325-28
  • [Book] Mathematical models for dynamics and management of algal blooms in aquatic ecosystems2009

    • Author(s)
      Amemiya T, Serizawa H, Ssakajo T, Itoh K
    • Publisher
      in Aquatic Ecosystem Research Trends. Nova Scientific Publishers, Inc. (in press)
  • [Book] よみがえれ! 研究者魂-研究はアイデア, 寄り道, セレンディピティだ-2009

    • Author(s)
      雨宮隆, 他
    • Publisher
      丸全株式会社(印刷中)
  • [Book] 生態環境リスクマネジメントの基礎2007

    • Author(s)
      浦野紘平・松田裕之共編、伊藤公紀ら(17名)著
    • Total Pages
      157-172.185-194
    • Publisher
      オーム社
  • [Patent(Industrial Property Rights)] DNA 検出センサおよびDNA 検出方法2007

    • Inventor(s)
      伊藤公紀、菊池晋平
    • Industrial Property Rights Holder
      国立大学法人横浜国立大学
    • Industrial Property Number
      特許,特願2007-212151
    • Filing Date
      2007-08-16
  • [Patent(Industrial Property Rights)] 光導波路型DNA センサおよびDNA 検出方法2006

    • Inventor(s)
      伊藤公紀、片岡淳司
    • Industrial Property Rights Holder
      国立大学法人横浜国立大学
    • Industrial Property Number
      特許,特願2008-70214
    • Filing Date
      2006-09-13

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Published: 2010-06-10   Modified: 2016-04-21  

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