Memory and transmission of genomic instability induced by ionizing

2008 Fiscal Year Final Research Report

• Project/Area Number: 18310040
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Osaka Prefecture University
• Principal Investigator: KODAMA Seiji Osaka Prefecture University, 産学官連携機構, 教授 (00195744)
• Co-Investigator(Renkei-kenkyūsha): SHIRAISHI Kazunori 大阪府立大学, 産学官連携機構, 助教 (40347513)
• Project Period (FY): 2006 – 2008
• Keywords: 放射線 / ゲノム不安定化 / 染色体異常 / 非標的影響 / 染色体移入 / 微小核融合法 / サブテロメア

Research Abstract

本研究は、放射線によるゲノム不安定化がいかにして子孫細胞に伝搬されるのかを明らかにするものである。放射線に被ばくしていないヒト染色体をマウス細胞に移入して得られた12種の細胞では、染色体構造異常頻度が10%を越える細胞は1つも無かった(0%)。一方、被ばくしたヒト染色体をマウス細胞に移入して得られた19種の細胞では、染色体構造異常が10%を越える細胞は8種(42%)みられた。また、予め再構成がある染色体は、被ばくすると不安定化しやすいことが分かった。以上の結果は、放射線により被ばく染色体に不安定化の原因が付与され、それ自身がゲノム不安定化を子孫細胞に伝搬すること、また、予め再構成が生じている染色体は被ばく後不安定化するポテンシャルが大きいことを示唆している。

Research Products

• [Journal Article] Introduction of a normal human chromosome 8 corrects abnormal phenotypes of Werner syndrome cells immortalized by expressing an hTERT gene (2009)
  • Author(s): Ariyoshi, K., Suzuki, K., Goto, M., Ishizaki, K., Watanabe, M., and Kodama, S.
  • Journal Title: J. Radiat. Res (in press)
  • Peer Reviewed
• [Journal Article] p^<53> status-dependent sensitization of human tumor cells to hyperthermia by plant flavonol (2008)
  • Author(s): Hamamoto, T., Suzuki, K., Yamauchi, M., Kodama, S., Sasaki, H., and Watanabe, M.
  • Journal Title: Int. J. Hyperthermia 24 Pages: 415-424
  • Peer Reviewed
• [Journal Article] Growth of persistent foci of DNA damage checkpoint factors is essential for amplification of G1 checkpoint signaling (2008)
  • Author(s): Yamauchi, M., Oka, Y., Yamamoto, M., Niimura, K., Uchida, M., Kodama, S., Watanabe, M., Sekine, I., Yamashita, S., and Suzuki, K.
  • Journal Title: DNA Repair 7 Pages: 405-417
  • Peer Reviewed
• [Journal Article] Different involvement of radical species in irradiated and bystander cells (2008)
  • Author(s): Harada, T., Kashino, G., Suzuki, K., Matsuda, N., Kodama, S. and Watanabe, M.
  • Journal Title: Int. J. Radiat. Biol 84 Pages: 809-814
  • Peer Reviewed
• [Journal Article] Transmission of genomic instability from a single irradiated human chromosome to the progeny of unirradiated cells (2007)
  • Author(s): Mukaida, N., Kodama, S., Suzuki, K., Oshimura, M., and Watanabe, M.
  • Journal Title: Radiat. Res Pages: 675-681
  • Peer Reviewed
• [Journal Article] Increased chromosome instability and accumulation of DNA double-strand breaks in Werner syndrome cells (2007)
  • Author(s): Ariyoshi, K., Suzuki, K., Goto, M., Watanabe, M., and Kodama, S.
  • Journal Title: J. Radiat. Res 48 Pages: 219-231
  • Peer Reviewed
• [Journal Article] Effective suppression of bystander effects by DMSO treatment of irradiated CHO cells (2007)
  • Author(s): Kashino, G., Prise, K. M., Suzuki, K., Matsuda, N., Kodama, S., Suzuki, M., Nagata, K., Kinashi, Y., Masunaga, S., Ono, K., and Watanabe, M.
  • Journal Title: J. Radiat. Res 48 Pages: 327-333
  • Peer Reviewed
• [Journal Article] Radiation induced bystander signals are independent of DNA damage and DNA repair capacity of the irradiated cells (2007)
  • Author(s): Kashino, G., Suzuki, K., Matsuda, N., Kodama, S., Ono, K., Watanabe, M., and Prise, K. M.
  • Journal Title: Mutat. Res 619 Pages: 134-138
  • Peer Reviewed
• [Journal Article] Early response of neural stem/progenitor cells after X-ray irradiation in vitro (2007)
  • Author(s): Kato, T., Kanemura, Y., Shiraishi, K., Miyake, J., Kodama, S., and Hara, M.
  • Journal Title: Neurorep 18 Pages: 895-900
  • Peer Reviewed
• [Journal Article] The chromosome network for biodosimetry in Japan (2007)
  • Author(s): Yoshida, M. A., Hayata, I., Tateno, H., Tanaka, K., Sonta, S., Kodama, S., Kodama, Y., and Sasaki, M. S.
  • Journal Title: Radiat. Measure 42 Pages: 1125-1127
  • Peer Reviewed
• [Journal Article] Correlation of malignant phenotypes of human tumour cell lines with augmented expression of Hsp72 protein measured by laser scanning cytometry (2007)
  • Author(s): Hamamoto, T., Suzuki, K., Kodama, S., Sasaki, H., Abe, K., Hayashi, T., Watanabe, M.
  • Journal Title: Int. J. Hyperthermia 23 Pages: 367-370
  • Peer Reviewed
• [Journal Article] Interstitial chromatin alteration causes persistent p^<53> activation involved in the radiation-induced senescence-like growth arrest (2006)
  • Author(s): Suzuki, M., Suzuki, K., Kodama, S., and Watanabe, M.
  • Journal Title: Biochem. Biophys. Res. Comm 340 Pages: 145-150
  • Peer Reviewed
• [Journal Article] Phosphorylated histone H2AX foci persist on rejoined mitotic chromosomes in normal human diploid cells exposed to ionizing radiation (2006)
  • Author(s): Suzuki, M., Suzuki, K., Kodama, S., and Watanabe, M.
  • Journal Title: Radiat. Res 165 Pages: 269-276
  • Peer Reviewed
• [Journal Article] Qualitative and quantitative analysis of phosphorylated ATM foci induced by low-dose ionizing radiation (2006)
  • Author(s): Suzuki, K., Okada, H., Yamauchi, M., Oka, Y., Kodama, S., and Watanabe, M.
  • Journal Title: Radiat. Res 165 Pages: 499-504
  • Peer Reviewed
• [Journal Article] Histone H2AX phosphorylation in normal human cells irradiated with focused ultrasoft X-rays : evidence for chromatin movement during repair (2006)
  • Author(s): Hamada, N., Shettino, G., Kashino, G., Valid, M., Suzuki, K., Kodama, S., Vojnovic, B., Folkard, M., Watanabe, M.
  • Journal Title: Radiat. Res 166 Pages: 31-38
  • Peer Reviewed
• [Journal Article] Non-specific detection of the centrosomes by antibodies recognizing phosphorylated ATM at serine 1981 (2006)
  • Author(s): Suzuki, K., Morimoto, M., Yamauchi, M., Yoshida, H., Kodama, S., Tsukamoto, K., and Watanabe, M.
  • Journal Title: Cell Cycle 5 Pages: 1008-1009
  • Peer Reviewed
• [Presentation] ヒト線維芽細胞の老化過程におけるDNA二重鎖切断の蓄積 (2008)
  • Author(s): 有吉健太郎, 白石一乗, 鈴木啓司, 後藤眞, 渡邉正己, 児玉靖司
  • Organizer: 日本環境変異原学会第37回大会
  • Place of Presentation: 沖縄
  • Year and Date: 20081204-06
• [Presentation] Induction of chromosomal instability by non-targeted effect of ionizing radiation (2008)
  • Author(s): Seiji Kodama
  • Organizer: NIRS Workshop on Cytogenetic Biodosimetry for Asia and 46^<th> ISTC Japan Workshop NIRS-ISTC Workshop on Cytogenetic Biodosimetry
  • Place of Presentation: Chiba
  • Year and Date: 20081157-28
• [Presentation] DMSOによる放射線防護効果はDNA-PK依存的修復に依存する (2008)
  • Author(s): 菓子野元郎, 漆原あゆみね児玉靖司, 小林純也,劉勇, 鈴木実, 増永慎一郎, 木梨友子. 渡邉正己, 小野公二
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州市
  • Year and Date: 20081119-21
• [Presentation] DNA損傷チェックポイントは細胞老化を介して発がんを抑制する (2008)
  • Author(s): 児玉靖司
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州市
  • Year and Date: 20081119-21
• [Presentation] Selective segregation of chromosomes in neural stem cells : re-examination of the immortal strand hypothesis (2008)
  • Author(s): Seiji Kodama, and Kazunori Shiraishi
  • Organizer: The 7^<th> Japan-France Workshop on Radiation Biology
  • Place of Presentation: Chiba
  • Year and Date: 20081014-16
• [Presentation] Accumulation of DNA double-strand breaks in senescent Werner syndrome and normal fibroblast cells (2008)
  • Author(s): Kentaro Ariyoshi, Keiji Suzuki, Makoto Goto, Masami Watanabe, and Seiji Kodama
  • Organizer: Ataxia-Telangiectasia Workshop 2008
  • Place of Presentation: Shiga
  • Year and Date: 20080422-26
• [Presentation] A Role of Chromosomal Instability in Carcinogenesis and Cellular Senescence (2008)
  • Author(s): Seiji Kodama
  • Organizer: An International Symposium of Kyoto University RRI
  • Place of Presentation: Kumatori
  • Year and Date: 2008-12-18
• [Presentation] 放射線による遅延性染色体異常誘発効果はヒト6番染色体と8番染色体では異なる (2007)
  • Author(s): 縄田寿克, 白石一乗, 松本真里, 押村光雄, 児玉靖司
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 千葉
  • Year and Date: 20071114-17
• [Presentation] マウス神経幹細胞は古い鋳型DNA鎖を選択的に保持する (2007)
  • Author(s): 永木恵美, 白石一乗, 原正之, 児玉靖司
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 千葉
  • Year and Date: 20071114-17
• [Presentation] 神経幹細胞の放射線感受性 (2007)
  • Author(s): 白石一乗, 有吉健太郎, 縄田寿克, 渡邉早苗, 久保喜平, 米澤司郎, 児玉靖司
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 千葉
  • Year and Date: 20071114-17
• [Presentation] 照射SHE細胞中の遅発性長寿命ラジカルは過酸化水素から生成する (2007)
  • Author(s): 宮崎将芳, 原田明, 児玉靖司, 渡邉正己, 熊谷純
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 千葉
  • Year and Date: 20071114-17
• [Presentation] 放射線誘導長寿命ラジカルの突然変異誘導への関与 (2007)
  • Author(s): 田苗祐二, 菓子野元郎, 熊谷純, 鈴木啓司, 児玉靖司, 渡邉正己
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 千葉
  • Year and Date: 20071114-17
• [Presentation] 放射線発がん初期過程のジェネテイクスとエピジェネテイクス (2007)
  • Author(s): 鈴木啓司, 児玉靖司, 渡邉正己
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 千葉
  • Year and Date: 20071114-17
• [Presentation] 放射線発がんの原因となる染色体異数化を誘導する機構 (2007)
  • Author(s): 渡邉正己, 吉居華子, 鈴木啓司, 児玉靖司
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 千葉
  • Year and Date: 20071114-17
• [Presentation] 放射線誘発mitotic catastropheによる細胞死の分子メカニズム (2007)
  • Author(s): 鈴木啓司, 山内基弘, 児玉靖司, 渡邉正己
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 千葉
  • Year and Date: 20071114-17
• [Presentation] 放射線生物学で意味ある線量域における点突然変異誘発遅発性長寿命ラジカルのESR直接観測 (2007)
  • Author(s): 熊谷純, 原田明, 宮崎将芳, 金森竜一, 吉川枝里, 児玉靖司, 渡邉正己
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 千葉
  • Year and Date: 20071114-17
• [Presentation] Transmission of genomic instability from a single irradiated human chromosome to the progeny of unirradiated cells (2007)
  • Author(s): Seiji Kodama, Naoki Mukaida, Hisakatsu Nawata, Kentaro Ariyoshi, Sanae Watanabe, Kazunori Shiraishi, Keiji Suzuki, Mitsuo Oshimura, and Masami Watanabe
  • Organizer: 13th International Congress of Radiation Research
  • Place of Presentation: San Francisco
  • Year and Date: 20070708-12
• [Presentation] Network for cytogenetic biodosimetry in Japan (2007)
  • Author(s): Japan, Mitsuaki A. Yoshida, Isamu Hayata, Hiroyuki Tateno, Kimio Tanaka, Shunichi Sonta, Seiji Kodama, Yoshiaki Kodama, and Masao S. Sasaki
  • Organizer: 13th International Congress of Radiation Research
  • Place of Presentation: San Francisco
  • Year and Date: 20070708-12
• [Presentation] Main route of radiation-carcinogenesis is DNA damage-independent pathway (2007)
  • Author(s): Masami Watanabe, Hanako Yoshii, Kimiko Watanabe, Keiji Suzuki, Seiji Kodama, and Jun Kumagai
  • Organizer: 13th International Congress of Radiation Research
  • Place of Presentation: San Francisco
  • Year and Date: 20070708-12
• [Presentation] Direct ESR measurement of novel slow-releasing radicals those might be responsible for delayed mutation induction in 4 Gy g-irradiated Syrian golden hamster embryonic cells (2007)
  • Author(s): Jun Kumagai, Akira Harada, Ryuichi Kanamori, Eri Yoshikawa, Masayoshi Miyazaki, Seiji Kodama, and Masami Watanabe
  • Organizer: 13th International Congress of Radiation Research
  • Place of Presentation: San Francisco
  • Year and Date: 20070708-12
• [Presentation] Increased chromosome instability and accumulation of DNA double-strand breaks in Werner syndrome cells (2007)
  • Author(s): Kentaro Ariyoshi, Kazunori Shiraishi, Keiji Suzuki, Makoto Goto, Masami Watanabe, and Seiji Kodama
  • Organizer: 13th International Congress of Radiation Research
  • Place of Presentation: San Francisco
  • Year and Date: 20070708-12
• [Presentation] Amplification of ATM-dependent checkpoint signals coupled with DNA double strand break repair (2007)
  • Author(s): Keiji Suzuki, Motohiro Yamauchi, Seiji Kodama, and Masami Watanabe
  • Organizer: 13th International Congress of Radiation Research
  • Place of Presentation: San Francisco
  • Year and Date: 20070708-12
• [Presentation] Amplification of G1 checkpoint signaling by growth of IR-induced foci (2007)
  • Author(s): Motohiro Yamauchi, Yasuyoshi Oka, Seiji Kodama, Masami Watanabe, and Keiji Suzuki
  • Organizer: 13th International Congress of Radiation Research
  • Place of Presentation: San Francisco
  • Year and Date: 20070708-12
• [Presentation] 非標的染色体変異と発がん (2007)
  • Author(s): 児玉靖司
  • Organizer: 日本環境変異原学会公開シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2007-05-26
• [Presentation] 放射線発がん-直接と間接の相違- (2007)
  • Author(s): 児玉靖司
  • Organizer: 京都大学原子炉実験所第41回学術講演会
  • Place of Presentation: 大阪
  • Year and Date: 2007-01-22
• [Presentation] Werner症候群細胞におけるDNA二重鎖切断の蓄積亢進によるテロメア不安定化 (2006)
  • Author(s): 吉健太郎, 渡邉早苗, 白石一乗, 鈴木啓司, 後藤真, 渡邉正己, 児玉靖司
  • Organizer: 日本環境変異原学会
  • Place of Presentation: 堺
  • Year and Date: 20061120-21
• [Presentation] 電離放射線による次世代マウスでの遣伝的不安定性誘導は精子期に生じる (2006)
  • Author(s): 白石一乗, 丹羽太貫, 児玉靖司
  • Organizer: 日本環境変異原学会
  • Place of Presentation: 堺
  • Year and Date: 20061120-21
• [Presentation] DNA-PKによるテロメアDNA末端安定性の制御 (2006)
  • Author(s): 吉田弘美, 鈴木啓司, 児玉靖司, 渡邉正己
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 札幌
  • Year and Date: 20060906-08
• [Presentation] DNA二重鎖切断修復過程と共役したDNA損傷シグナルの増幅 (2006)
  • Author(s): 鈴木啓司, 山内基弘, 児玉靖司, 渡邉正己
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 札幌
  • Year and Date: 20060906-08
• [Presentation] ウェルナー症候群細胞におけるテロメア脆弱性 (2006)
  • Author(s): 有吉健太郎, 児玉靖司, 白石一乗, 鈴木啓司, 後藤真, 渡邉正己
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 札幌
  • Year and Date: 20060906-08
• [Presentation] ATM特異的阻害によるDNA損傷シグナル増幅のG1アレストにおける役割の解明 (2006)
  • Author(s): 山内基弘, 鈴木啓司, 山本将史, 内田素行, 新村浩一, 児玉靖司, 渡邉正己
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 札幌
  • Year and Date: 20060906-08
• [Presentation] マウス放射線適応応答は2つの機構によりもたらされる (2006)
  • Author(s): 白石一乗, 有吉健太郎, 縄田寿克, 渡邉早苗, 久保喜平, 米澤司郎, 児玉靖司
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 札幌
  • Year and Date: 20060906-08
• [Presentation] 酸化ストレスに対する鋭敏な指標としてのテロメアシグナル異常 (2006)
  • Author(s): 児玉靖司, 渡邉早苗, 有吉健太郎, 白石一乗, 縄田寿克, 渡邉正己
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 札幌
  • Year and Date: 20060906-08
• [Presentation] 哺乳類細胞中の長寿命ラジカルと重イオン照射効果 (2006)
  • Author(s): 原田明, 熊谷純, 江原将文, 上野昭子, 野島久美恵, 児玉靖司, 渡邉正己
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 札幌
  • Year and Date: 20060906-08
• [Presentation] Telomere biology : implications for radiation carcinogenesis (2006)
  • Author(s): Seiji Kodama, Kentaro Ariyoshi, Sanae Watanabe, Kazunori Shiraishi,, Masami Watanabe
  • Organizer: The Second Nagasaki Symposium of International and Consortium for Medical Care of Hibakusha Radiation Life Science
  • Place of Presentation: Nagasaki
  • Year and Date: 20060726-28
• [Presentation] Growth of IR-induced foci and G1 checkpoint (2006)
  • Author(s): Motohiro Yamauchi, Keiji Suzuki, Seiji Kodama, Masami Watanabe
  • Organizer: The Second Nagasaki Symposium of International Consortium for Medical Care of Hibakusha and Radiation Life Science
  • Place of Presentation: Nagasaki
  • Year and Date: 20060726-28
• [Presentation] 放射線発がん機構の新説-DNAは標的ではない-
  • Author(s): 渡邉正己, 吉井華子, 渡邉喜美子, 鈴木啓司, 児玉靖司
  • Organizer: 日本環境変異原学会
  • Place of Presentation: 堺
  • Year and Date: 10061120-21
• [Book] 「どうして放射線によってがんが起こるのか」みんなのくらしと放射線(分担)(編)「みんなのくらしと放射線」 (2008)
  • Author(s): 児玉靖司
  • Total Pages: 54-66
  • Publisher: 大阪公立大学共同出版会
• [Book] エックス線の生体に予える影響」エックス線取扱の基礎・演習(分担)((編)飯田敏行) (2007)
  • Author(s): 児玉靖司
  • Total Pages: 29-42,89-104
  • Publisher: 電子科学研究所
• [Book] 放射線による遣伝的影響はどのように推定されるのか・最近の放射線による遺伝的リスクの評価法は従来のものとどこが違うのか」低線量放射線と健康影響(分担), (編)(土居雅広, 神田玲子, 米原英典, 吉永信治, 島田義也) (2007)
  • Author(s): 児玉靖司
  • Total Pages: 151-152,162-164
  • Publisher: 医療科学社
• [Remarks] 大阪府立大学・産学官連機構・先端科学イノベーションセンター・放射線生産科学研究室のホームページ
  • URL: http://chokai.riast.osakafu-u.ac.jp%7Ehousya6/home.html