Physicochemical and biochemical study of radiation induced DNA damage

2008 Fiscal Year Final Research Report

• Project/Area Number: 18310041
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: YOKOYA Akinari Japan Atomic Energy Agency, 先端基礎研究センター, 研究主幹 (10354987)
• Co-Investigator(Kenkyū-buntansha): FUJII Kentaro 独立行政法人日本原子力研究開発機構, 先端基礎研究センター, 研究員 (00360404) ; WATANABE Ritsuko 独立行政法人日本原子力研究開発機構, 原子力機構基礎工学部門, 研究副主幹 (10360439) ; AKAMATSU Ken 独立行政法人日本原子力研究開発機構, 原子力機構基礎工学部門, 研究副主幹 (70360401) ; SHIKAZONO Naoya 独立行政法人日本原子力研究開発機構, 先端基礎研究センター, 研究主幹 (10354961) ; URUSHIBARA Ayumi 独立行政法人日本原子力研究開発機構, 先端基礎研究センター, 任期付研究員 (80391275) ; NOGUCHI Miho 独立行政法人日本原子力研究開発機構, 先端基礎研究センター, 研究員 (40455283)
• Research Collaborator: O'NEILL Peter University of Oxford, Prof. ; NIKJOO Hooshang NASA, Dr.
• Project Period (FY): 2006 – 2008
• Keywords: 放射線生物影響 / クラスターDNA損傷 / LET / 電子常磁性共鳴(EPR) / シンクロトロン放射 / DNA鎖切断 / 塩基損傷 / 塩基除去修復酵素

Research Abstract

放射線による生物影響の主要な要因のひとつとされる難修復性のDNA多重損傷(クラスターDNA損傷)は、空間電離密度の指標であるLETが100keV/μm以上のイオンビーム照射時に顕著に生じること、さらに大型シンクロトロン放射施設から得られる高いエネルギー分解能の軟X線を用いてDNA分子中の特定元素の内殻電離効果を調べた結果、酸素のK殻励起エネルギーを超えると塩基損傷頻度が増加することを突き止めた。

Research Products

• [Journal Article] The yield, processing, and biological consequences of clustered DNA(Invited Review) (2009)
  • Author(s): Shikazono N,. Noguchi, M., Fujii, K., Urushibara, A. and Yokoya, A.
  • Journal Title: J. Radiat. Res. 50 Pages: 27-36
  • Peer Reviewed
• [Journal Article] Estimation of yields of hydroxyl radicals in water under various energy heavy ions (2009)
  • Author(s): aguchi, M., Kimura, A., Watanabe, R. and Hirota, K.
  • Journal Title: Radiat. Res. 171 Pages: 254-263
  • Peer Reviewed
• [Journal Article] Biological dose estimation for charged-particle therapy using an improved PHITS code coupled with a microdosimetric kinetic model (2009)
  • Author(s): Sato, T., Kase, Y., Watanabe, R., Niita, K. and Sihver, L.
  • Journal Title: Radit. Res. 171 Pages: 107-117
  • Peer Reviewed
• [Journal Article] Unrepairable DNA damage site composed of clustered lesions along ion particle tracks (2008)
  • Author(s): Yokoya, A., Shikazono, N., Fujii, K., Ushigome, T., Suzuki, M., Urushibara, A. and Watanabe, R.
  • Journal Title: Proceedings of the 27th Symposium on Materials Science and Engineering Research of Ion Beam Technology (in Hosei Univ.)(Invited paper) Pages: 1-8
• [Journal Article] Studies of soft-X-ray -induced Auger effect on the induction of DNA damage (2008)
  • Author(s): Yokoya, A., Fujii, K., Shikazono, N., Akamatsu, K., Urushibara, A., and Watanabe, R.
  • Journal Title: Int. J. Radiat. Biol. 84 Pages: 1069-1081
  • Peer Reviewed
• [Journal Article] Characterization of lesions induced in linear-formed plasmid DNA by valence ionization and Auger decay at carbon, nitrogen and oxygen (2008)
  • Author(s): Akamatsu, K., Fujii, K., and Yokoya, A.
  • Journal Title: Int. J. Radiat. Biol. 84 Pages: 1082-1092
  • Peer Reviewed
• [Journal Article] Induction of single strand breaks, and base lesions in plasmid DNA films induced by carbon, nitrogen, and oxygen KLL Auger process (2008)
  • Author(s): Fujii, K., Yokoya, A., and Shikazono, N.
  • Journal Title: Induction of single strand breaks, and base lesions in plasmid DNA films induced by carbon, nitrogen, and oxygen KLL Auger process 84 Pages: 1104-1111
  • Peer Reviewed
• [Journal Article] Can Monte Carlo track structure codes Reveal reaction mechanism in DNA damage and improve radiation therapy? (2008)
  • Author(s): Nikjoo, H., Emfietzoglou, D., Watanabe, R. and Uehara, S.
  • Journal Title: Radiat. Phys. Chem. 77 Pages: 1270-1279
  • Peer Reviewed
• [Journal Article] ASR2007 International symposium on "Charged Particle an Photon Interactions with Matter", November 6-9, 2007. (2008)
  • Author(s): Katsumura, Y., Lin, M., Yokoya, A. and Hatano, Y.
  • Journal Title: Radiat. Phys. Chem. 77 Pages: 1119
• [Journal Article] DNA Damage induced by direct effect of radiation(Review) (2008)
  • Author(s): Yokoya, A., Shikazono, N., Fujii, K., Urushibara, A., Akamatsu, K. and Watanabe, R.
  • Journal Title: Radiat. Phys. Chem. 77 Pages: 77
  • Peer Reviewed
• [Journal Article] X-ray absorption spectrum for guanosine-5-monophosphate in water solution in the vicinity of the nitrogen K-edge observed in free liquid jet in vacuum (2008)
  • Author(s): Ukai, M., Yokoya, A., Fujii, K. and Saitoh, Y.
  • Journal Title: Radiat. Phys. Chem. 77 Pages: 1265-1269
  • Peer Reviewed
• [Journal Article] LET dependence of the yield of single-, double-strand breaks and base lesions in fully hydrated plasmid DNA films by 4He2+ ion irradiation (2008)
  • Author(s): Urushibara, A., Shikazono, N., O'Neill, P., Fujii, K., Wada, S. and Yokoya, A.
  • Journal Title: Int. J. Radiat. Biol. 84 Pages: 23-33
• [Journal Article] Biological effect of clustered DNA damage (2006)
  • Author(s): Shikazono, N., Urushibara, A., Fujii, K. and Yokoya, A.
  • Journal Title: Radiat. Biol. Res. Comm. 41 Pages: 409-423
• [Journal Article] The roles of specific glycosylases in determining the mutagenic consequences of clustered DNA base damage (2006)
  • Author(s): Shikazono, N., Pearson, C., O'Neill, P. and Thacker, J.
  • Journal Title: Nucleic Acids Res. 34 Pages: 3730-3738
• [Journal Article] Yields of strand breaks and base lesions induced by soft X-rays in plasmid DNA (2006)
  • Author(s): Yokoya, A., Fujii, K., Ushigome, T., Shikazono, N., Urushibara, A. and Watanabe, R.
  • Journal Title: Radiat. Prot. Dosim. 122 Pages: 86-88
  • Peer Reviewed
• [Journal Article] DNA damage induced by the direct effect of He ion particles (2006)
  • Author(s): Urushibara, A., Shikazono, N. Watanabe, R., Fujii, K., O'Neill, P. and Yokoya, A.
  • Journal Title: Radiat. Prot. Dosim. 122 Pages: 163-165
  • Peer Reviewed
• [Journal Article] Processing of thymine glycol in a clustered DNA damage site: mutagenic or cytotoxic
  • Author(s): Bellon, S., Shikazono, N., Cunniffe, S., Lomax, M., O'Neill, P.
  • Journal Title: Nucleic Acids Res. (in press)
  • Peer Reviewed
• [Journal Article] Induction of DNA strand breaks, base lesions and clustered damage sites in hydrated plasmid DNA films by ultrasoft X-rays around the phosphorus K edge
  • Author(s): Yokoya, A., Cunniffe, S.M.T., Watanabe, R., Kobayashi, K. and O'Neill, P.
  • Journal Title: Radiat. Res. (in press)
  • Peer Reviewed
• [Journal Article] EPR study of radiation damage to DNA irradiated with synchrotron soft X-rays around nitrogen and oxygen K-edge
  • Author(s): Yokoya, A., Fujii, K., Fukuda, Y. and Ukai, M.
  • Journal Title: Radiat. Phys. Chem. (in press)
  • Peer Reviewed
• [Journal Article] Spectral change in X-ray absorption near edge structure of DNA thin films irradiated with monochromatic soft X-rays
  • Author(s): Fujii, K. and Yokoya, A.
  • Journal Title: Radiat. Phys. Chem. (in press)
  • Peer Reviewed
• [Journal Article] Synchrotron radiation photoelectron studies for primary radiation effects using a liquid water jet in vacuum: total and partial photoelectron yields for liquid water near the oxygen K-edge
  • Author(s): Ukai, M., Yokoya, A., Nonaka, Y., Fujii, K. and Saitoh, Y.
  • Journal Title: Radiat. Phys. Chem. (in press)
  • Peer Reviewed
• [Presentation] プラスミドDNAに対する高LET放射線による非DSB性多重SSB誘発の可能性 (2008)
  • Author(s): 横谷明徳
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州国際会議場、北九州
  • Year and Date: 20081119-21
• [Presentation] 鎖切断と脱塩基部位からなるクラスターDNA損傷の生物効果 (2008)
  • Author(s): 鹿園直哉
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州国際会議場、北九州
  • Year and Date: 20081119-21
• [Presentation] 非二本鎖切断型クラスター損傷(8-oxoG と鎖切断)の突然変異誘発 (2008)
  • Author(s): 野口実穂
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州国際会議場、北九州
  • Year and Date: 20081119-21
• [Presentation] 軟X線照射によって乾燥プラスミドDNAに生じる分子損傷の収率 (2008)
  • Author(s): 藤井健太郎
  • Organizer: 第51回放射線化学討論会
  • Place of Presentation: 産業技術総合研究所、つくば市
  • Year and Date: 20081015-17
• [Presentation] 放射線により誘発されるDNA損傷の空間分布と難修復性 (2008)
  • Author(s): 横谷明徳
  • Organizer: 第3回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎シティーギャラリー、高崎(招待講演)
  • Year and Date: 20081009-10
• [Presentation] 軟X線によってDNA分子内に生じるサイト選択的分子変化 (2008)
  • Author(s): 藤井健太郎
  • Organizer: 第2回分子科学討論会
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 20080924-27
• [Presentation] 微視的エネルギー付与分布と生物影響 (2008)
  • Author(s): 渡辺立子
  • Organizer: 京都大学原子炉専門研究会「放射線影響量と生物影響の新概念-細胞内の線量分布と生物影響-
  • Place of Presentation: 京都大学原子炉研究所、熊取(招待講演)
  • Year and Date: 20080910-11
• [Presentation] EPR Study of radiation damage to DNA irradiated with synchrotron soft X-rays around nitrogen and oxygen K-edge (2008)
  • Author(s): A. Yokoya
  • Organizer: The 2nd Asia-Pacific Symposium on Radiation Chemistry (APSRC-2008)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20080829-0901
• [Presentation] A quantitative analysis of the yield of radiation-induced DNA strand breaks in hydrated plasmids (2008)
  • Author(s): N. Shikazono
  • Organizer: The 2nd Asia-Pacific Symposium on Radiation Chemistry (APSRC-2008)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20080829-0901
• [Presentation] Spectral change in X-ray absorption near edge structure of DNA thin films irradiated with monochromatic soft X-rays (2008)
  • Author(s): K. Fujii
  • Organizer: The 2nd Asia-Pacific Symposium on Radiation Chemistry (APSRC-2008)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20080829-0901
• [Presentation] Lethality and mutagenesis of E. coli cells irradiated with soft X-rays (2008)
  • Author(s): T. Suzaka
  • Organizer: The 2nd Asia-Pacific Symposium on Radiation Chemistry (APSRC-2008)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20080829-0901
• [Presentation] A novel technique using DNA denaturation to detect prompt and enzymatically induced single strand breaks produced in a clustered damage site (2008)
  • Author(s): A. Yokoya
  • Organizer: The 10th International Workshop on Radiation Damage to DNA
  • Place of Presentation: Urabandai, Fukushima, Japan
  • Year and Date: 20080608-12
• [Presentation] DNA damage spectra in ultrasoft X-ray region: effect of inner-shell ionization of carbon, nitrogen and oxygen atoms on the damage induction (2008)
  • Author(s): K. Fujii
  • Organizer: The 10th International Workshop on Radiation Damage to DNA
  • Place of Presentation: Urabandai, Fukushima, Japan
  • Year and Date: 20080608-12
• [Presentation] Mutagenic potential of 8-oxo-7,8-dihydroguanine depends on the location of a single strand break within a cluster (2008)
  • Author(s): M. Noguchi
  • Organizer: 10th International Workshop on Radiation Damage to DNA
  • Place of Presentation: Urabandai, Fukushima, Japan
  • Year and Date: 20080608-12
• [Presentation] Processing of clustered DNA damage site in escherichia coli (2008)
  • Author(s): N. Shikazono
  • Organizer: The 10th International Workshop on Radiation Damage to DNA
  • Place of Presentation: Urabandai, Fukushima, Japan
  • Year and Date: 20080608-12
• [Presentation] イオンビームにより誘発される難修復性のクラスターDNA損傷 (2008)
  • Author(s): 横谷明徳
  • Organizer: 第27回法政大学イオンビーム工学研究所シンポジウム
  • Place of Presentation: 法政大学、小金井(招待講演)
  • Year and Date: 2008-12-10
• [Presentation] 放射線によるゲノム損傷とその生体修復のせめぎあい (2008)
  • Author(s): 横谷明徳
  • Organizer: 第11回茨城大学イブニングセミナー
  • Place of Presentation: 茨城大学、水戸(招待講演)
  • Year and Date: 2008-10-23
• [Presentation] DNA損傷と放射線生物影響 (2008)
  • Author(s): 横谷明徳
  • Organizer: 平成20年度弥生研究会~放射線化学とその周辺から~
  • Place of Presentation: 東京大学東海村キャンパス、東海村、茨城県
  • Year and Date: 2008-07-29
• [Presentation] クラスターDNA損傷による突然変異誘発 (2008)
  • Author(s): 鹿園 直哉
  • Organizer: 平成20年度弥生研究会~放射線化学とその周辺から~
  • Place of Presentation: 東京大学東海村キャンパス、東海村、茨城県
  • Year and Date: 2008-07-29
• [Presentation] 非2本鎖切断型クラスター損傷(8-oxoG と鎖切断)の突然変異誘発 (2007)
  • Author(s): 野口実穂
  • Organizer: 放射線影響学会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 20071114-17
• [Presentation] 8-oxoGとDHTからなるクラスターDNA損傷のプロセシング経路 (2007)
  • Author(s): 鹿園直哉
  • Organizer: 放射線影響学会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 20071114-17
• [Presentation] 放射線によりプラスミドDNAの両鎖に生じた非DSB性多重SSBのDNAの変性を利用した新しい定量法 (2007)
  • Author(s): 横谷明徳
  • Organizer: 放射線影響学会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 20071114-17
• [Presentation] DNA damage induced by direct effect of radiation (2007)
  • Author(s): A. Yokoya
  • Organizer: The 7th International Symposium on Advanced Science Research: Charged Particle and Photon Interaction with Matter
  • Place of Presentation: JAEA-Tokai, Ibaraki, Japan (Invited paper)
  • Year and Date: 20071106-09
• [Presentation] Biological consequences of bistanded clustered DNA damage in Escherichia coli (2007)
  • Author(s): N. Shikazono
  • Organizer: The 7th International Symposium on Advanced Science Research: Charged Particle and Photon Interaction with Matter
  • Place of Presentation: JAEA-Tokai, Ibaraki, Japan
  • Year and Date: 20071106-09
• [Presentation] Mutagenic potential of non-DSB clustered damage containing 8-oxoG and single strand break (2007)
  • Author(s): M. Noguchi
  • Organizer: The 7th International Symposium on Advanced Science Research: Charged Particle and Photon Interaction with Matter
  • Place of Presentation: JAEA-Tokai, Ibaraki, Japan
  • Year and Date: 20071106-09
• [Presentation] Strand breaks and base lesions in dry plasmid DNA films induced by monochromatic ultrasoft X-rays (2007)
  • Author(s): K. Fujii
  • Organizer: The 7th International Symposium on Advanced Science Research: Charged Particle and Photon Interaction with Matter
  • Place of Presentation: JAEA-Tokai, Ibaraki, Japan
  • Year and Date: 20071106-09
• [Presentation] 放射光単色軟X線によりDNA中に生じる主鎖切断収量及び修復酵素をプローブとして用いた塩基損傷の定量 (2007)
  • Author(s): 藤井健太郎
  • Organizer: 放射線化学討論会
  • Place of Presentation: 京都大学宇治キャンパス
  • Year and Date: 20071010-12
• [Presentation] Mutagenic potential of clustered DNA damage site in Escherichia coli (2007)
  • Author(s): N. Shikazono
  • Organizer: The 13th International Congress of Radiation Research
  • Place of Presentation: San Francisco, USA
  • Year and Date: 20070708-12
• [Presentation] A novel technique using DNA denaturation to analyze clustered DNA damage sites induced by densely ionizing radiation (2007)
  • Author(s): A. Yokoya
  • Organizer: The 13th International Congress of Radiation Research
  • Place of Presentation: San Francisco, USA
  • Year and Date: 20070708-12
• [Presentation] Induction of strand breaks and base lesions in dry plasmid DNA films induced by 270-560 eV ultrasoft X-rays (2007)
  • Author(s): K. Fujii
  • Organizer: The 13th International Congress of Radiation Research
  • Place of Presentation: San Francisco, USA
  • Year and Date: 20070708-12
• [Presentation] Studies of soft-X-rayinduced Auger effect on the induction of DNA damage (2007)
  • Author(s): A. Yokoya
  • Organizer: The 6th Auger Symposium -An International Symposium on Physical, Molecular, Cellular and Medical Aspects of Auger Processes
  • Place of Presentation: Boston, USA. (Invited paper)
  • Year and Date: 20070705-07
• [Presentation] Induction of strand breaks, base lesions, and clustered damage in dry plasmid DNA films induced by carbon, nitrogen and oxygen KLL Auger process (2007)
  • Author(s): K. Fujii
  • Organizer: The 6th Auger Symposium - An International Symposium on Physical, Molecular, Cellular and Medical Aspects of Auger Processes
  • Place of Presentation: Boston, USA
  • Year and Date: 20070705-07
• [Presentation] Auger and photoelectron effects on strand breaks and base lesions in plasmid DNA irradiated with ultrasoft X-rays (2007)
  • Author(s): K. Akamatsu
  • Organizer: The 6th Auger Symposium- An International Symposium on Physical, Molecular, Cellular and Medical Aspects of Auger Processes
  • Place of Presentation: Boston, USA
  • Year and Date: 20070705-07
• [Presentation] DNAプリン塩基中の酸素及び窒素K殻励起により生じる短寿命のラジカルのEPR測定 (2007)
  • Author(s): 横谷明徳
  • Organizer: 第20回日本放射光学会年会・放射光科学合同シンポジウム
  • Place of Presentation: 広島
  • Year and Date: 20070112-14
• [Presentation] 光子及びイオン粒子照射により生じるDNA鎖切断及び塩基損傷の収率のLET依存性 (2006)
  • Author(s): 横谷明徳
  • Organizer: 日本放射線影響学会第49回大会
  • Place of Presentation: 札幌
  • Year and Date: 2006-09-06
• [Presentation] 非二本鎖切断型クラスターDNA損傷の生物効果 (2006)
  • Author(s): 鹿園直哉
  • Organizer: 日本放射線影響学会第49回大会
  • Place of Presentation: 札幌
  • Year and Date: 2006-09-06
• [Presentation] 軟X線(270-560eV)照射により乾燥DNA中に生じる鎖切断、塩基損傷及びクラスター損傷の照射エネルギー依存性 (2006)
  • Author(s): 藤井健太郎
  • Organizer: 日本放射線影響学会第49回大会
  • Place of Presentation: 札幌
  • Year and Date: 2006-09-06
• [Presentation] 大腸菌における8-オキソグアニンとチミングリコールから成るクラスターDNA損傷の突然変異誘効果 (2006)
  • Author(s): 漆原あゆみ
  • Organizer: 日本放射線影響学会第49回大会
  • Place of Presentation: 札幌
  • Year and Date: 2006-09-06
• [Presentation] DNA鎖切断末端構造の酵素反応速度論敵研究 (2006)
  • Author(s): 赤松憲
  • Organizer: 日本放射線影響学会第49回大会
  • Place of Presentation: 札幌
  • Year and Date: 2006-09-06
• [Presentation] Mutagenic potential of clustered DNA damage site in Escherichia
  • Author(s): N. Shikazono
  • Organizer: The 9th International Workshop on Radiation Damage to DNA, May 14-17, 2006, Antalya, Turkey. 41. A. Yokoya, DNA damages induced by ion particles and photons with various energies. The 9th International Workshop on Radiation Damage to DNA, May 14-17, 2006, Antalya, Turkey.
• [Book] 放射線によるDNA損傷誘発過程のモンテカルロシミュレーション、「放射線化学のすすめ」日本放射線化学会編 (2006)
  • Author(s): 渡辺立子
  • Total Pages: 86-88
  • Publisher: 学会出版センター