Analysis of differential gene expression profiles induced by genotoxic and non-genotoxic carcinogens

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18310047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Aoyama Gakuin University
• Principal Investigator: FURIHATA Chie Aoyama Gakuin University, College of Science and Engineering, Professor (70012755)
• Co-Investigator(Kenkyū-buntansha): WATANABE Takashi Aoyama Gakuin University, College of Science and Engineering, Assistant Professor (50406815) ; SUZUKI Takayoshi National Institute of Health Sciences, 遺伝子細胞医薬部, Section Chief (30226526)
• Project Period (FY): 2006 – 2007
• Keywords: Toxicogenomics / gene expression profile / DNA microarray / quantitative real-time PCR / genotoxic / non-genotoxic / carcinogen / mouse liver

Research Abstract

The aim of this study is clarifying differential gene expression profiles and action mechanisms between genotoxic and non-genotoxic carcinogens in the target organ. The following results were obtained.
1)Differential gene expression profiles of 2 N-nitroso carcinogenic compounds, diethylnitrosamine and dipropylnitrosamine, and a non-genotoxic carcinogenic compound, phenobarbital, in mouse liver at 4 h and 28 days after administration determined with DNA microarray and quantitative real-time PCR.
2) Dose-dependent alteration in the gene expression in mouse liver induced by diethylnitrosamine and ethylnitrosourea at 4 h and 28 days after administration determined by quantitative real-time PCR.
3) Time-dependent gene expression profiles at 4, 16, 20, 24, and 48 h after administration of a genotoxic carcinogen, chrysene, in mouse liver determined with quantitative real-time PCR.
4) Differential gene expression profiles induced by 4 genotoxic carcinogens [quinoline, N-nitroso morpholine, 4-dimethyl (amino) azobenzene and 2, 4-diaminotoluene], and 2 non-genotoxic carcinogens (clofibrate and dichlorobenzene) in mouse liver at 4 and 48 h after administration determined with quantitative real-time PCR examined as training set.
5) Differential gene expression profiles induced by 4 genotoxic carcinogens [3, 4-(methylnitrosamino)-a-(3-pyridyl)-1-butanone, 2-actylamonofluorene, urethane, diisopropanolnitrosamine] and 3 non-genotoxic carcinogens [di(2-ethylhexyl)phthalate, dichloro-diphenyl-trichloroethane and furan] in mouse liver at 4 and 48 h after administration determined with quantitative real-time PCR examined as validation set.

Research Products

• [Journal Article] Differential gene expression induced by two N-nitroso carcinogens,phenobarbital and ethanol in mouse liver examined with oligonucleotide microarray and quantitative real-time PCR (2007)
  • Author(s): Takashi Watanabe, Kaori Tobe, Yutaka Nakachi, Yasumitsu Kondoh, Madoka Nakajima, Shuichi Hamada, Chiaki Namiki, Takayoshi Suzuki, Satoru Maeda, Ayami Tadakuma, Mikiya Sakurai, Yuko Arai, Hyogo Atsushi, Masako Hoshino, Tomoko Tashiro, Hisashi Ito, Hiroshige Inazumi, Yoshiyuki Sakaki, Hideo Tashiro, Chie Furihata
  • Journal Title: Genes and Environment 29 Pages: 115-127
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differential gene expression induced by two N-nitroso carcinogens, phenobarbital and ethanol in mouse liver examined with oligonucleotide microarray and quantitative real-time PCR (2007)
  • Author(s): Takashi, Watanabe, Kaori, Tobe, Yutaka, Nakachi, Yasumitsu, Kondoh, Madoka, Nakajima, Shuichi, Hamada, Chiaki, Namiki, Takayoshi, Suzuki, Satoru, Maeda, Ayami, Tadakuma, Mikiya, Sakurai, Yuko, Arai, Hyogo, Atsushi, Masako, Hoshino, Tomoko, Tashiro, Hisashi, Ito, Hiroshige, Inazumi, Yoshiyuki, Sakaki, Hideo, Tashiro, Chie, Furihata
  • Journal Title: Genes and Environment 29 Pages: 115-127
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 遺伝子傷害性肝発がん物質のマウス肝臓における遺伝子発現解析 (2007)
  • Author(s): 多田隈 英未、櫻井 幹也、渡辺 貴志、鈴木 孝昌、濱田 修一、平山 道朝、真田 尚和、鈴木 洋、中嶋 圓、大信田 系裕、佐久間 智宏、降旗 千恵
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ジエチルニトロソアミンによるマウス肝臓における遺伝子発現変化の用量反応性 (2007)
  • Author(s): 新井 祐子、多田隈 英未、渡辺 貴志、濱田 修一、鈴木 孝昌、中嶋 圓、降旗 千恵
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 遺伝子傷害性肝発がん物質Chryseneのマウス肝臓における遺伝子発現解析 (2007)
  • Author(s): 櫻井 幹也、多田隈 英未、花原 泉、渡辺 貴志、鈴木 孝昌、降旗 千恵
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Analysis of gene expression profiles in mouse liver Induced by genotoxic carcinogens (2007)
  • Author(s): A, Tadakuma, M, Sakurai, T, Watanabe, T, Suzuki, S, Hamada, M, Hirayama, H, Sanada, H, Suzuki, M, Nakajima, K, Ohshita, T, Sanada, C, Furihata
  • Organizer: The 30th Annual meeting of the Molecular Biology Society of Japan & The 80th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Dose-dependent changes in gene expression profiles in mouse liver induced with diethyl-nitrosamine (2007)
  • Author(s): Y, Arai, A, Tadakuma, T, Watanabe, S, Hamada, T, Suzuki, M, Nakajima, C, Furihata
  • Organizer: The 30th Annual meeting of the Molecular Biology Society of Japan & The 80th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Time-dependent gene expression profiles in mouse liver induced with a genotoxic carcinogen, chrysene (2007)
  • Author(s): M, Sakurai, A, Tadakuma, I, Hanahara, T, Watanabe, T, Suzuki, C, Furihata
  • Organizer: The 30th Annual meeting of the Molecular Biology Society of Japan & The 80th Annual Meeting of the Japanese Biochemical Society
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 2007-12-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Collaborative study of JEMS/MMS/Toxicogenomics:Quantutative real-time PCR analysis on mouse liver carcinogens (2007)
  • Author(s): C Furihata, T Watanabe, A Tadakuma, M Sakurai, T Suzuki, S, Hamada, K Narumi, M Nakajima, A Koeda, T Sakuma, K Oshida, H Sanada and M Hirayama
  • Organizer: 1st Asian Conference on Environmental Mutagens&36th Annual Meeting of the Japanese Mutagen Society
  • Place of Presentation: Kitakyushu International Conference Center
  • Year and Date: 2007-11-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Collaborative study on the Toxicogenomics in JEMS/MMS II:High-throughput qPCR analysis by TagMan Low Density Array (2007)
  • Author(s): T Suzuki, T Koizumi, D Prabha, Y Luan, M Honma, S Hamada, M Nakajima, T Watanabe, C Furihata
  • Organizer: 1st Asian Conference on Environmental Mutagens&36th Annual Meeting of the Japanese Mutagen Society
  • Place of Presentation: Kitakyushu International Conference Center
  • Year and Date: 2007-11-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Collaborative study of JEMS/MMS/Toxicogenomics Quantutative real-time PCR analysis on mouse liver carcinogens (2007)
  • Author(s): C, Furihata, T Watanabe, A, Tadakuma, M, Sakurai, T, Suzuki, S, Hamada, K, Narumi, M, Nakajima, A, Koeda, T, Sakuma, K, Oshida, H, Sanada and M, Hirayama
  • Organizer: 1st Asian Conference on Environmental Mutagens & 36th Annual Meeting of the Japanese Mutagen Society
  • Place of Presentation: Kitakyushu International Conference Center
  • Year and Date: 2007-11-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Collaborative study on the Toxicogenomics in JEMS/MMS II : High-throughput qPCR analysis by TaqMan Low Density Array (2007)
  • Author(s): T, Suzuki, T, Koizumi, D, Prabha, Y, Luan, M, Honma, S, Hamada, M, Nakajima, T, Watanabe, C, Furihata
  • Organizer: 1st Asian Conference on Environmental Mutagens & 36th Annual Meeting of the Japanese Mutagen Society
  • Place of Presentation: Kitakyushu International Conference Center
  • Year and Date: 2007-11-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Differentially expressed genes in mouse liver induced by N-nitroso carcinogenic compounds, phenobarbital and ethanol (2006)
  • Author(s): C Furihata, K Tobe, T Watanabe, S Maeda, M Hirayama, M Harada, M Nakajima, S Hamada, C Namiki, T Suzuki, Y Nakachi, Y Kondoh, T Tashiro and C Furihata
  • Organizer: The 20th IUBMB Congress and 11th FAOBMB Congress
  • Place of Presentation: Kyoto International Conference Hall
  • Year and Date: 2006-06-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Dose-dependent alteration in the expression of mouse hepatic genesinduced by diethylnitrosamine and ethylnitrosourea determined by quantitative RT-PCR (2006)
  • Author(s): T Watanabe, S Maeda, M Hirayama, S Hamada, C Namiki, M Nakajima, T Suzuki and C Furihata
  • Organizer: The 20th IUBMB Congress and 11th FAOBMB Congress
  • Place of Presentation: Kyoto International Conference Hall
  • Year and Date: 2006-06-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Dose-dependent alteration in the expression of mouse hepatic genes induced by diethylnitrosamine and ethylnitrosourea determined by quantitative RT-PCR (2006)
  • Author(s): T, Watanabe, S, Maeda, M, Hirayama, S, Hamada, C, Namiki, M, Nakajima, T, Suzuki and C, Furihata
  • Organizer: The 20th IUBMB Congress and 11th FAOBMB Congress
  • Place of Presentation: Kyoto International Conference Hall
  • Year and Date: 2006-06-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.chem.aoyama.ac.jp/Chem/ChemHP/Furihatalab/