2008 Fiscal Year Final Research Report
Creation of novel photosynthetic microorganism for degradation of PCB in oligotrophic environment
| Project/Area Number |
18310050
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Ibaraki University |
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Principal Investigator |
KIMURA Shigenobu Ibaraki University, 工学部, 教授 (90291608)
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| Co-Investigator(Renkei-kenkyūsha) |
SENDA Toshiya 産業技術総合研究所, バイオメディシナル情報研究センター, 主任研究員 (30272868)
KAHINO Yasuhiro 兵庫県立大学, 大学院・生命理学研究科, 准教授 (20221872)
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| Research Collaborator |
FUKUDA Masao 長岡技術科学大学, 工学部, 教授 (20134512)
NISHIZAWA Akito 茨城大学, 産学連携イノベーション創成機構, 非常勤研究員 (30466643)
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| Project Period (FY) |
2006 – 2008
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| Keywords | 環境修復技術 / バイオレメディエーション / PCB / シアノバクテリア / 光合成 / タンパク質工学 / 基質特異性 / X 線結晶構造解析 |
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| Research Abstract |
水や土壌中などに残留するポリ塩化ビフェニル(PCB)の汚染除去には、ビフェニル分解微生物を用いた生物的環境浄化が有効である.本研究では、PCB 分解細菌Pseudomonas sp. KKS102 株の分解酵素系タンパク質BphA1~A4 遺伝子を光合成微生物であるシアノバクテリア(ラン藻)に組み込んで、これまで困難であった貧栄養環境下でも光エネルギーを用いてビフェニル化合物を分解できる新規光合成微生物を創出した.
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[Journal Article] Molecular mechanism of the redox-dependent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S] ferredoxin2007
Author(s)
Senda, M., Kishigami, S., Kimura, S., Fukuda, M., Ishida, T., Senda, T
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Journal Title
J.Mol.Biol 373
Pages: 382-400
Peer Reviewed
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[Presentation] Enzymatic properties of NADH/NADPH-specificity inverted BphA4 mutants from Pseudomonas sp. Strain KKS1022008
Author(s)
Nishizawa, A., Muramatsu, D., Kishigami, S., Kashino, Y., Senda, M., Senda, T., Fukuda, M., Kimura, S
Organizer
第31回日本分子生物学会年会 第81回日本生化学会大会 合同大会 (BMB2008)
Place of Presentation
神戸市神戸ポートアイランド
Year and Date
2008-12-12
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[Presentation] Molecular mechanism of the redox-dependent interaction between ferredoxin reductase and ferredoxin2008
Author(s)
Senda, M., Kimura, S., Fukuda, M., Ishida, T., Senda, T
Organizer
XXI Congress and Genaral Assenbly of the International Union of Crystallography (IUCr2008)
Place of Presentation
Grand Cube Osaka, Osaka
Year and Date
2008-08-28
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[Presentation] Inversion of NADH/NADPH-specificity of BphA4 from Pseudomonas sp. Strain KKS102 by substitutions of Glu175 and Gln1772008
Author(s)
Nishizawa, A., Muramatsu, D., Kimura, S., Kishigami, S., Senda, M., Senda, T., Fukuda, M
Organizer
16th Symposium on Flavins and Flavoproteins 2008
Place of Presentation
Palacio de Congressos, Jaca, Spain
Year and Date
2008-06-09
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[Presentation] Molecular Mechanism of the redox-dependent interaction between NADH-dependent ferredoxin reductase and NADH/NAD^<+>2008
Author(s)
Senda, M., Kimura, S., Fukuda, M., Ishida, T., Senda, T
Organizer
16th Symposium on Flavins and Flavoproteins 2008
Place of Presentation
Palacio de Congressos, Jaca, Spain
Year and Date
2008-06-09
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[Book] Inversion of NADH/NADPH-specificity of BphA4 from Pseudomonas sp. strain KKS102 by substitutions of Glu^<175> and Gln^<177>. in Flavins and Flavoproteins 2008 (Fargo, S., Gomez-Moreno, C., Medina, M. eds)2008
Author(s)
Nishizawa, A., Muramatsu, D., Kishigami, S., Senda, M., Senda, T., Fukuda, M., and Kimura, S
Total Pages
273-278
Publisher
Prensas Universitarias de Zaragoza, Spain
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[Book] Prensas Universitarias de Zaragoza, Spain2008
Author(s)
Senda, M., Kimura, S., Fukuda, M., Ishida, T., and Senda, T
Total Pages
113-118
Publisher
Molecular mechanism of the redox-depsndent interaction between NADH-dependent ferredoxin reductase and Rieske-type [2Fe-2S] ferredoxin. In Flavins and Flavoproteins 2008 (Fargo, S., Gomez-Moreno, C., Medina, M. eds)
