2007 Fiscal Year Final Research Report Summary
Membrane Protein Chip with Liposomes
| Project/Area Number |
18310092
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | The University of Tokyo |
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Principal Investigator |
LEPIOUFLE Bruno The University of Tokyo, Institute of Industrial Science, Visiting Scholar (60422432)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Shoji The University of Tokyo, Institute of Industrial Science, Associate Professor (90343110)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Microfluidic Devices / Membrane Proteins / Quantitative Analysis / MEMS / Lab on a Chip / Lipid Bilavers |
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| Research Abstract |
Membrane proteins play very important roles in cells (e.g. recognition or transportation of molecules). They are also useful in various industrial fields, including next-generation diagnosis techniques, drug discovery, and highly sensitive ion-channel-based biosensors.
In this research, we investigated a method toward membrane protein chips : an array of single-species-specific membrane proteins reconstituted into the surfaces of giant liposomes. In the method, we try to prepare monodisperse giant vesicles by (1) patterning lipid films on ITO substrates with a dry lift-off process and subsequently applying voltages between the substrates, or by (2)"blowing" a planar lipid bilayer and deforming it into vesicles (like blowing soap bubbles). We have also developed a simple method to make an array of the lipid bilayers on a microfluidic chip.
We believe that these devices are useful for an efficient and rapid analysis of single-species-specific membrane proteins.
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