2008 Fiscal Year Final Research Report
Tissue-specific processing pathway of precursor proteins deduced from peptidome analysis
| Project/Area Number |
18310138
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied genomics
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
MINAMINO Naoto National Cardiovascular Center Research Institute, 薬理部, 部長 (50124839)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Kazuki 国立循環器病センター(研究所), 薬理部, 部長 (80260313)
OSAKI Tsukasa 国立循環器病センター(研究所), 薬理部, 室員 (60380565)
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| Project Period (FY) |
2006 – 2008
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| Keywords | ペプチドーム / プロセシング / 質量分析 / 下垂体 / 甲状腺C細胞 |
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| Research Abstract |
Some proteins are post-translationally converted to peptides,which in turn function as hormones and neuropeptides. This conversion reaction is called "processing", and tissue-specifically performed by a set of processing enzymes. Peptides generated from the same precursor protein are often different in each tissue, and hard to predict from genome information. In this study, we prepared intact peptides from endocrine-type tissues and cells, and comprehensively identified them by a renovated peptidomic technology. Based on accumulated peptide data of these cells and tissues, we provide a principal approach to deduce a processing pathway of a particular precursor protein as well as peptides generated from the protein in target cells or tissues.
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[Journal Article] Distribution of neuroendocrine regulatory peptide-1 and -2, and proteolytic processing of their precursor VGF protein in the rat.2010
Author(s)
E. Mishiro-Satoh, K. Sasaki, T. Matsuo, H. Kageyama, H. Yamaguchi, Y. Date, M. Matsubara, T. Ishizu, K. Yoshizawa-Kumagaye, Y. Satomi, T. Takao, S. Shioda, M. Nakazato, N. Minamino
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Journal Title
J. Neurochem 114
Pages: 1097-1106
Peer Reviewed
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[Journal Article] Peptidomic identification and biological validation of neuroendocrine regulatory peptide-1 and -2. *: equally contributed2007
Author(s)
H. Yamaguchi*, K. Sasaki*, Y. Satomi*, T. Shimbara, H. Kageyama, M. S. Mondal, K. Toshinai, Y. Date, L. J. Gonzalez, S. Shioda, T. Takao, M, Nakazato, N. Minamino
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Journal Title
J. Biol. Chem 282
Pages: 26354-26360
Peer Reviewed
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