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2008 Fiscal Year Final Research Report

Development of the method for proteome analysis based on a novel chemical derivatization specific to C-termini of proteins

Research Project

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Project/Area Number 18310146
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionNara Women's University

Principal Investigator

NAKAZAWA Takashi  Nara Women's University, 理学部, 教授 (30175492)

Co-Investigator(Renkei-kenkyūsha) KUYAMA Hiroki  大阪大学, 蛋白質研究所寄付研究部門, 准教授 (60437332)
NISHIMURA Osamu  大阪大学, 蛋白質研究所寄付研究部門, 教授 (10374125)
GASKELL Simonj  University of Manchester, Director of the Michael Barber Centre for Mass Spectrometry, Professor [Vice President (Research) of the [Vice President (Research) of the University]
Project Period (FY) 2006 – 2008
Keywordsプロテオミクス
Research Abstract

タンパク質のC末端アミノ酸配列解析法の開発のために、本研究ではペプチドの選択的C末端誘導体化の反応条件を最適化した結果、簡単なアミド化において収率を平均60%以上にすることに成功した。この誘導体化と新規N末端標識に基づくC末端ペプチド単離法を組み合わせることで、C末端が翻訳後修飾されたペプチドのマトリックス支援レーザー脱離イオン化質量分析法(MALDI-MS)によるアミノ酸配列解析を可能にした。

  • Research Products

    (27 results)

All 2008 2007 2006 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (9 results) Book (1 results) Remarks (2 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 2 results)

  • [Journal Article] Selective isolation of N-terminal peptides from proteins and their de novo sequencing by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry without regard to unblocking or blocking of N-terminal amino acids2008

    • Author(s)
      Minoru Yamaguchi, Daisuke Nakayama, Keisuke Shima, Hiroki Kuyama, Eiji Ando, Taka-aki Okamura, Norikazu Ueyama, Takashi Nakazawa, Shigemi Norioka, Osamu Nishimura, and Susumu Tsunasawa
    • Journal Title

      Rapid Commun. Mass Spectrom 22

      Pages: 3313-3319

    • Peer Reviewed
  • [Journal Article] Determination of pKa values of individual histidine residues in proteins using mass spectrometry2008

    • Author(s)
      Masaru Miyagi and Takashi Nakazawa
    • Journal Title

      (Accelerated article) Anal Chem. 80

      Pages: 6481-6487

    • Peer Reviewed
  • [Journal Article] Terminal proteomics : N-and C-terminal analyses for high-fidelity identification of proteins using mass spectrometry2008

    • Author(s)
      Takashi Nakazawa, Minoru Yamaguchi, Taka-aki Okamura, Eiji Ando, Osamu Nishimura, and Susumu Tsunasawa
    • Journal Title

      Proteomics 8

      Pages: 673-685

    • Peer Reviewed
  • [Journal Article] Specific isolation of N-terminal fragments from proteins and their high-fidelity de novo sequencing2007

    • Author(s)
      Minoru Yamaguchi, Takashi Obama, Hiroki Kuyama, Daisuke Nakayama, Eiji Ando, Taka-aki Okamura, Norikazu Ueyama, Takashi Nakazawa, Shigemi Norioka, Osamu Nishimura, and Susumu Tsunasawa
    • Journal Title

      Rapid Commun. Mass Spectrom 21

      Pages: 3329-3326

    • Peer Reviewed
  • [Journal Article] Simultaneous detection of N-terminal fragment ions in a protein mixture using a ruthenium(II) complex2007

    • Author(s)
      Akihiro Ito, Taka-aki Okamura, Hitoshi Yamamoto, Norikazu Ueyama, Minoru Yamaguchi, Hiroki Kuyama, Eiji Ando, Susumu Tsunasawa, Kojiro Ake, Ryoji Masui, Seiki Kuramitsu, Takashi Nakazawa, and Shigemi Norioka
    • Journal Title

      Rapid Commu. Mass Spectrom 21

      Pages: 2647-2653

    • Peer Reviewed
  • [Journal Article] High sequence-coverage detection of proteolytic peptides using a bis(terpyridine)ruthenium(II) complex2007

    • Author(s)
      Akihiro Ito, Taka-aki Okamura, Ken Masui, Maki Kaneko, Ryoji Masui, Kojiro Ake, Seiki Kuramitsu, Minoru Yamaguchi, Hiroki Kuyama, Eiji Ando, Shigemi Norioka, Takashi Nakazawa, Susumu Tsunasawa, Hitoshi Yamamoto, and Norikazu Ueyama
    • Journal Title

      Analyst 132

      Pages: 358-364

    • Peer Reviewed
  • [Journal Article] Tautomerism of histidine 64 associated with proton-transfer in catalysis of carbonic anhydrase2007

    • Author(s)
      Hideto Shimahara, Takuya Yoshida, Yasutaka Shibata, Masato Shimizu, Yoshimasa Kyogoku, Fumio Sakiyama, Takashi Nakazawa, Shin-ichi Tate, Shin-ya Ohki, Takeshi Kato, Hozumi Moriyama, Ken-ichi Kishida, Yasuo Tano, Tadayasu Ohkubo, and Yuji Kobayashi
    • Journal Title

      J. Biol. Chem. 282

      Pages: 9646-9656

    • Peer Reviewed
  • [Journal Article] Enhancement of MALDI-MS spectra of Cterminal peptides by the modification of proteins via an active ester generated in situ from an oxazolone2006

    • Author(s)
      Minoru Yamaguchi, Mutsumi Oka, Kimiko Nishida, Mayu Ishida, Ayako Hamazaki, Hiroki Kuyama, Eiji Ando, Taka-aki Okamura, Norikazu Ueyama, Shigemi Norioka, Osamu Nishimura, Susumu Tsunasawa, and Takashi Nakazawa
    • Journal Title

      Anal. Chem. 78

      Pages: 7861-7869

    • Peer Reviewed
  • [Journal Article] Chemistry in proteomics : an interplay between classical methods in chemical modification of proteins and mass spectrometry at the cutting edge2006

    • Author(s)
      Takashi Nakazawa
    • Journal Title

      Current Proteomics 3

      Pages: 33-54

    • Peer Reviewed
  • [Journal Article] A new approach for detecting C-terminal amidation of proteins and peptides by mass spectrometry in conjunction with chemical derivatization

    • Author(s)
      Hiroki Kuyama, Chihiro Nakajima, Takashi Nakazawa, Osamu Nishimura, and Susumu Tsunasawa
    • Journal Title

      Proteomics, 9 (in press)

    • Peer Reviewed
  • [Presentation] Isolation and mass spectrometric sequencing of C-terminal peptides based on exhaustive amidation of proteins2008

    • Author(s)
      Mariko Nakagawa, Minoru Yamaguchi, Hiroki Kuyama, Chihiro Nakajima, Susumu Tsunasawa, Osamu Nishimura, Eiji Ando, and Takashi Nakazawa
    • Organizer
      BMB2007(第31回日本分子生物学会・第81回日本生化学会合同大会)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2008-12-12
  • [Presentation] Isolation of C-terminal peptides of proteins by exhaustive amidation followed by proteolytic digestion for sequencing with mass spectrometry2008

    • Author(s)
      Mariko Nakagawa, Minoru Yamaguchi, Hiroki Kuyama, Chihiro Nakajima, Eiji Ando, Susumu Tsunasawa, Osamu Nishimura, and Takashi Nakazawa
    • Organizer
      17^<th> Meeting of Methods in Proteins Structure Analysis
    • Place of Presentation
      北海道大学
    • Year and Date
      2008-08-27
  • [Presentation] Isolation of C-terminal peptides of proteins by exhaustive amidation followed by proteolytic digestion for mass spectrometric sequencing2008

    • Author(s)
      Mariko Nakagawa, Minoru Yamaguchi, Hiroki Kuyama, Eiji Ando, Susumu Tsunasawa, Osamu Nishimura, and Takashi Nakazawa
    • Organizer
      56^<th> ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Denver, Colorado, USA
    • Year and Date
      2008-06-02
  • [Presentation] MALDI-MSを用いたタンパク質のC末端ヒドラジド化によるアミノ酸配列解析法2007

    • Author(s)
      中島ちひろ, 中川真理子, 水谷文, 辻智恵美, 岡むつみ, 山口実, 綱澤進, 西村紀, 中沢隆
    • Organizer
      BMB2007(第30回日本分子生物学会・第80回日本生化学会合同大会)
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-12-12
  • [Presentation] C-Terminal Derivatization of proteins via hydrazides for sequencing with MALDI-MS2007

    • Author(s)
      Takashi Nakazawa, Minoru Yamaguchi, Mariko Nakagawa, Mutsumi Oka, Chihiro Nakajima, Hiroki Kuyama, Eiji Ando, Daisuke Nakayama, Taka-aki Okamura, Osamu Nishimura, and Susumu Tsunasawa
    • Organizer
      55^<th> ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Indianapolis, Indiana, USA
    • Year and Date
      2007-06-05
  • [Presentation] MALDI質量分析のためのC末端ヒドラジド化に基づくペプチドの標識法2007

    • Author(s)
      中川真理子,中島ちひろ, 山口実, 岡むつみ, 辻智恵美, 九山浩樹, 安藤英治, 岡村高明, 綱澤進, 西村紀, 中沢隆
    • Organizer
      第7回日本蛋白質科学会年会
    • Place of Presentation
      東北大学
    • Year and Date
      2007-05-24
  • [Presentation] C-Terminal activation of peptides with an active ester via an oxazolone2006

    • Author(s)
      Mutsumi Oka, Mariko Yamamoto, Chihiro Nakajima, Chiemi Tsuji, Mariko Nakagawa, Minoru Yamaguchi, Kimiko Nishida, Susumu Tsunasawa and Takashi Nakazawa
    • Organizer
      Int Conference of 43^<rd> Japanese Peptide Symposium/4^<th> Peptide Engineering Meeting
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2006-11-05
  • [Presentation] MALDI mass spectrometric analysis of proteins by charge tagging at the C-terminus2006

    • Author(s)
      Takashi Nakazawa, Minoru Yamaguchi, Kimiko Nishida, Mutsumi Oka, Ayako Hamazaki, Hiroki Kuyama, Eiji Ando, Taka-aki Okamura, Norikazu Ueyama, Shigemi Norioka, and Susumu Tsunasawa
    • Organizer
      20^<th> IUBMB International Congress of Biochemistry and Molecular Biology
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2006-06-22
  • [Presentation] C-Terminal charge-tagging of proteins for the sequencing with MALDI-MS2006

    • Author(s)
      Takashi Nakazawa, Minoru Yamaguchi, Kimiko Nishida, Mutsumi Oka, Ayako Hamazaki, Hiroki Kuyama, Eiji Ando, Taka-aki Okamura, Norikazu Ueyama, Shigemi Norioka, and Susumu Tsunasawa
    • Organizer
      55^<th> ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Seattle, Washington, USA
    • Year and Date
      2006-05-30
  • [Book] 「金属錯体と金属タンパク質の質量分析」生命元素事典(桜井弘編)第4章, 2項〔7〕2006

    • Author(s)
      中沢隆、矢野重信
    • Total Pages
      407-411
    • Publisher
      オーム社
  • [Remarks]

    • URL

      http://www.chem.nara-wu.ac.jp/~nakazawa/

  • [Remarks] セミナーでの講演Takashi Nakazawa. "Protein Chemistry and Enzymology as Fundamentals for Proteomics", in Proteomics and Functional Genomics Group Special Seminar, 23^<rd> September 2008, Department of Veterinary Science, University of Liverpool, Liverpool, UK.

  • [Patent(Industrial Property Rights)] ペプチドのアミノ酸配列決定法2008

    • Inventor(s)
      山口実、安藤英治、乗岡茂巳、上山憲一、岡村高明、中澤隆
    • Industrial Property Rights Holder
      株式会社島津製作所
    • Industrial Property Number
      特許第4232660号
    • Acquisition Date
      2008-12-19
  • [Patent(Industrial Property Rights)] イソチオシアネート基を有する金属錯体、及びそれを用いるタンパク質のアミノ酸配列決定方法2008

    • Inventor(s)
      上山憲一、岡村高明、乗岡茂巳、中澤隆、山口実、安藤英治
    • Industrial Property Rights Holder
      株式会社島津製作所
    • Industrial Property Number
      特許第4211305号
    • Acquisition Date
      2008-11-07
  • [Patent(Industrial Property Rights)] Method for modifying protein or peptide C-terminal2007

    • Inventor(s)
      Takashi Nakazawa, Minoru Yamaguchi, Hiroki Kuyama, Eiji Ando, Norikazu Ueyama, Taka-aki Okamura, and Shigemi Norioka
    • Industrial Property Rights Holder
      Shimadzu Corporation
    • Industrial Property Number
      US 7, 294, 707 B2
    • Acquisition Date
      2007-11-13
    • Overseas
  • [Patent(Industrial Property Rights)] タンパク質又はペプチドのC末端修飾法、C末端固定化法、及び解析法2006

    • Inventor(s)
      中澤隆、岡むつみ、西田希実子、山口 実、九山浩樹、安藤英治、上山憲一、岡村高明、綱澤進
    • Industrial Property Rights Holder
      株式会社 島津製作所、国立大学法人奈良女子大学、国立大学法人大阪大学(JST特許出願支援 : #S2006-0043PTC)
    • Industrial Property Number
      特許, 特願2005-307831
    • Filing Date
      2006-10-20
    • Overseas
  • [Patent(Industrial Property Rights)] タンパク質のN末端フラグメントを選択的に回収する方法2006

    • Inventor(s)
      乗岡茂巳、上山憲一、岡村高明、中澤隆、山口実、安藤英治、綱澤進
    • Industrial Property Rights Holder
      株式会社島津製作所
    • Industrial Property Number
      特許第4120580号
    • Acquisition Date
      2006-05-09

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Published: 2010-06-10   Modified: 2016-04-21  

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