2008 Fiscal Year Final Research Report
Study of the methods for visualizing intracellular signaling molecules
| Project/Area Number |
18350045
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | The University of Tokyo (2007-2008)
Institute for Molecular Science (2006) |
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Principal Investigator |
OZAWA Takeaki The University of Tokyo, 大学院・理学系研究科, 教授 (40302806)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Masaki 東京大学, 大学院・理学系研究科, 助教 (00332271)
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| Project Period (FY) |
2006 – 2008
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| Keywords | 可視化 / バイオテクノロジー / 分析化学 / 生体分子 |
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| Research Abstract |
In the present study, we studied the development of optical probes for visualizing bio molecules using luciferases, GFP and its spectral variants based on protein reconstitution methods. Moreover, we generated new concepts for molecular imaging such as luciferase cyclization by protein splicing and spatiotemporal analysis of protein-protein interactions based on split luciferase complementation. These results are expected to contribute to resolving biological function in live cells.
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