Study of electron transfer on cell surface by self assembly mono layer

2008 Fiscal Year Final Research Report

• Project/Area Number: 18360459
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nuclear engineering
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: OHNUKI Toshihiko Japan Atomic Energy Agency, 先端基礎研究センター, 研究主席 (20354904)
• Co-Investigator(Kenkyū-buntansha): KOZAI Naofumi 日本原子力研究開発機構, 先端基礎研究センター, 研究副主幹 (80354877) ; SAKAMOTO Fuminori 日本原子力研究開発機構, 先端基礎研究センター, 研究副主幹 (60391273) ; SUZUKI Yoshinori 日本原子力研究開発機構, 先端基礎研究センター, 博士研究員 (20455281) ; TANAKA Kazuya 日本原子力研究開発機構, 先端基礎研究センター, 博士研究員 (60377992)
• Co-Investigator(Renkei-kenkyūsha): NANKAWA Takuya 日本原子力研究開発機構, バックエンド技術部, 研究員 (30370448) ; OZKAI Takuo 日本原子力研究開発機構, 先端基礎研究センター, 研究員 (50322673)
• Project Period (FY): 2006 – 2008
• Keywords: 自己組織化単分子層 / アクチノイド / 酸化還元 / 電子授受

Research Abstract

官能基を自発的に一分子層だけ配列させた金薄膜を電極として、細胞膜(SAM)-酵素-U間の電子授受機構を解明する研究を行った。その結果、U(VI)-有機酸錯体の還元挙動は錯形成定数の小さい場合にはU(IV)の沈殿を生じ、大きな場合には可溶性のU(IV)-有機酸を生じた。また、官能基として4-ピリジンに配位したU(VI)への電子授与を電気化学的に検出し、錯形成定数のおおよその値を得た。さらに、微生物レベルではU(VI)を還元できない酵母由来のチトクロームCを介したU(VI)への電子授与を捉えることに成功した。

Research Products

• [Journal Article] Modeling of the Interaction of Pu(VI) with the Mixture of Microorganism and Clay (2009)
  • Author(s): T. Ohnuki, T. Yoshida, T. Ozaki, K. Naofumi, F. Sakamoto, T. Nankawa, Y. Suzuki, A. J. Francis
  • Journal Title: J. Nucl. Scie. Technol. 46 Pages: 55-59
  • Peer Reviewed
• [Journal Article] Association of Uranyl Ions with Amino Functional Groups (2009)
  • Author(s): N. Kozai, T. Ohnuki
  • Journal Title: Chemistry Letters Vol. 38 Pages: 152-153
  • Peer Reviewed
• [Journal Article] Sorption of U(VI) on the 4-Mercaptopyridine Self-Assembled Monolayer (2008)
  • Author(s): T. Nankawa, Y. Suzuki, T. Ozaki, A. J. Francis, T. Ohnuki
  • Journal Title: J. Nucl. Sci. Tech. 45 Pages: 251-256
  • Peer Reviewed
• [Journal Article] Concurrent transformation of Ce(III) and formation of biogenic manganese oxides (2008)
  • Author(s): T. Ohnuki, T. Ozaki, T. Yoshida, N. Kozai, T. Nankawa, F. Sakamoto, T. Sakai, Y. Suzuki, A. J. Francis
  • Journal Title: Chem. Geology 253 Pages: 23-29
  • Peer Reviewed
• [Journal Article] Chemical speciation and association of plutonium with bacteria, kaolinite clay, and their mixture (2007)
  • Author(s): T. Ohnuki, T. Yoshida, T. Ozaki, N. Kozai, F. Sakamoto, T. Nankawa, Y. Suzuki, A.J. Francis
  • Journal Title: Environmental Science & Technology 41 Pages: 3134-3139
  • Peer Reviewed
• [Journal Article] Metal sorption to Pseudomonas fluorescens of pH, ionic strength and metal concentrations (2007)
  • Author(s): Y. Takenaka, T. Saito, S. Nagasaki, S. Tanaka, N. Kozai, T. Ohnuki
  • Journal Title: Geomicrobiology J. 24 Pages: 205-210
  • Peer Reviewed
• [Journal Article] Electrochemical Studies on Uranium in the Presence of Organic Acids (2007)
  • Author(s): Y. Suzuki, T. Nankawa, T. Ozaki, T. Ohnuki, A. J. Francis, Y. Enokida, I. Yamamoto
  • Journal Title: J. Nuclear Sci. Tech. 44 Pages: 1227-1232
• [Journal Article] Characterization of homoionic Fe2+-type montmorillonite: Potential chemical species of iron contaminant (2007)
  • Author(s): N. Kozai, K. Inada, Y. Adachi, S. Kawamura, Y. Kashimoto, T. Kozaki, S. Sato, T. Ohnuki, T. Sakai, T. Sato, M. Oikawa, F. Egusa, H. Mitamura
  • Journal Title: J. Solid State Chemistry 180 Pages: 2279-2289
  • Peer Reviewed
• [Journal Article] Protein Expression of Saccharomyces cerevisiae in Response to Uranium Exposure (2007)
  • Author(s): F. Sakamoto, T. Nankawa, N. Kozai, T. Fujii, H. Iefuji, A. J. Francis, T. Ohnuki,
  • Journal Title: J. Nucl. RadioChem. Sci. 8 Pages: 133-136
  • Peer Reviewed
• [Journal Article] Microbial Transformations of Plutonium (2007)
  • Author(s): A. J. Francis, T. Ohnuki
  • Journal Title: J. Nucl. RadioChem. Sci. 8 Pages: 121-126
  • Peer Reviewed
• [Journal Article] Degradation of Eu(III)-Malic Acid Complexes by Pseudomonas fluorescens (2006)
  • Author(s): T. Nankawa, Y. Suzuki, T. Ozaki, T. Ohnuki, A. J. Francis
  • Journal Title: J. Alloy Compounds 408-412 Pages: 1329-1333
  • Peer Reviewed
• [Journal Article] Interactions of rare earth elements with bacteria and organic ligands (2006)
  • Author(s): T. Ozaki, Y. Suzuki, T. Nankawa, T. Yoshida, T. Ohnuki, T. Kimura, A. J. Francis
  • Journal Title: J. Alloy Compounds 408-412 Pages: 1334-1338
• [Journal Article] Association of europium(III), americium(III), and curium(III) with cellulose, chitin, and chitosan (2006)
  • Author(s): T. Ozaki, T. Kimura, T. Ohnuki, A. Kirishima, T. Yoshida, H. Isobe, A. J. Francis
  • Journal Title: Environ. Toxicol. Chem. 25 Pages: 2051-2058
  • Peer Reviewed
• [Journal Article] Redox behavior of uranium in the presence of citric acid (2006)
  • Author(s): Y. Suzuki, T. Nankawa, T. Yoshida, T. Ozaki, T. Ohnuki, A.J. Francis, S. Tsushima, Y. Enokida, I. Yamamoto
  • Journal Title: Radiochimica Acta 94 Pages: 579-583
  • Peer Reviewed
• [Journal Article] Effects of ionic strength on the coordination of Eu(III) and Cm(III) to a Gram-negative bacterium Paracoccus denitrificans (2006)
  • Author(s): T. Ozaki, T. KIMURA,T. OHNUKI, A. J. FRANCIS
  • Journal Title: Radiochemica Acta 94 Pages: 715-722
  • Peer Reviewed
• [Presentation] Changes in chemical speciation of Ce(III) and its association with biogenic manganese oxides (2009)
  • Author(s): T. Ohnuki, N. Kozai, T. Nankawa, F. Sakamoto, Y. Suzuki, T. Tanaka, T. Sakai, A.J. Francis
  • Organizer: 237th ACS National Meeting & Exposition
  • Place of Presentation: Salt Lake City, USA
  • Year and Date: 20090322-26
• [Presentation] Bioreduction of U(VI)-organic complexes by Shewanella putrefaciens (2008)
  • Author(s): Y.Suzuki, N. Kozai, A.J. Francis, T. Ohnuki
  • Organizer: 7-th International Symposium for Subsurface Microbiology (ISSM2008)
  • Place of Presentation: Shizuoka, Japan
  • Year and Date: 20081016-21
• [Presentation] Microbial Impacts on the Migration of Actinides (2008)
  • Author(s): T. Ohnuki
  • Organizer: Workshop on Radionuclides Migration
  • Place of Presentation: Beijing, China
  • Year and Date: 20081012-14
• [Presentation] Mn酸化微生物とCe(III)との相互作用 (2008)
  • Author(s): 大貫敏彦、他3名
  • Organizer: 地球化学学会2008年年会
  • Place of Presentation: 東京、駒場
  • Year and Date: 20080917-19
• [Presentation] マンガン酸化物への4価プルトニウムの吸着と酸化 (2008)
  • Author(s): 田中万也、大貫敏彦
  • Organizer: 地球化学学会2008年年会
  • Place of Presentation: 東京、駒場
  • Year and Date: 20080917-19
• [Presentation] ShewanellaputrefaciensによるU(VI)の還元-有機酸錯体の還元 (2008)
  • Author(s): 東京、駒場
  • Organizer: 地球化学学会2008年年会
  • Place of Presentation: 東京、駒場
  • Year and Date: 20080917-19
• [Presentation] REDOX BEHAVIOR OF Ce(IV)/Ce(III) IN THE PRESENCE OF ORGANIC ACIDS (2008)
  • Author(s): Y. Suzuki, T. Nankawa, T. Yoshida, T. Ozaki, T. Ohnuki, A. J. Francis, Y. Enokida, I. Yamamoto
  • Organizer: Migration '07
  • Place of Presentation: Munchen
  • Year and Date: 20080826-31
• [Presentation] Interactions of Actinides with Microorganisms and Organic Ligands (2008)
  • Author(s): T. Ohnuki, T. Ozaki, F. Sakamoto, N. Kozai, T. Nankawa, Y. Suzuki, A. J. Francis
  • Organizer: Goldschmidt conference
  • Place of Presentation: Vancouber, Canada
  • Year and Date: 20080713-18
• [Presentation] Interaction between Paramecium bursaria and Europium(III) (2008)
  • Author(s): N. Kozai, T. Ohnuki
  • Organizer: Goldschmidt conference
  • Place of Presentation: Vancouber, Canada
  • Year and Date: 20080713-18
• [Presentation] Study of uranium(VI) association with cell surface of microorganisms using functional groups attached electrodes (2008)
  • Author(s): T. Ohnuki, T. Nankawa, Y. Suzuki
  • Organizer: Pu Future The Science
  • Place of Presentation: Dijon, France
  • Year and Date: 20080706-11
• [Presentation] MODELING OF THE INTERACTION OF Pu(VI) WITH THE MIXTURE OF MICROORGANISM AND CLAY (2007)
  • Author(s): T. Ohnuki, T. Ozaki, N. Kozai, F. Sakamoto, T. Nankawa, Y. Suzuki, A. J. Francis
  • Organizer: Migration '07
  • Place of Presentation: Munchen
  • Year and Date: 20070826-31
• [Presentation] ANALYSIS OF PROTEINS EXPRESSED IN Saccharomyces cerevisiae EXPOSED BY URANIUM(VI) (2007)
  • Author(s): F. Sakamoto, T. Nankawa, T. Ohnuki, A. J. Francis
  • Organizer: Migration '07
  • Place of Presentation: Munchen
  • Year and Date: 20070826-31
• [Presentation] DSORPTION OF URANIUM(VI) ON SILICA PARTICLES MODIFIED WITH PROTEIN (2007)
  • Author(s): N. Kozai, T. Nankawa, T. Ohnuki, A. J. Francis
  • Organizer: Migration '07
  • Place of Presentation: Munchen
  • Year and Date: 20070826-31
• [Presentation] Interactions of Heavy Elements with Microorganisms (2007)
  • Author(s): T. Ohnuki, T. Yoshida, T. Ozaki, F. Sakamoto, N. Kozai, T. Nankawa, Y. Suzuki, A. J. Francis
  • Organizer: Goldschmidt2007
  • Place of Presentation: Cologne
  • Year and Date: 20070819-26
• [Presentation] Reduction of U(VI) by Shewanella putrefaciens in the presence of organic acids (2007)
  • Author(s): Y. Suzuki, T. Nankawa, T. Ozaki, T. Ohnuki, A.J. Francis
  • Organizer: Goldschmidt2007
  • Place of Presentation: Cologne
  • Year and Date: 20070819-26
• [Presentation] Interaction of uranium(VI) with cell surface of microorganisms (2007)
  • Author(s): T. OHNUKI, T. OZAKI, T. NANKAWA, N. KOZAI, F. SAKAMOTO, Y. SUZUKI, A. J. FRANCIS
  • Organizer: 233rd American Chemical Society National Meeting & Exposition
  • Place of Presentation: Chicago
  • Year and Date: 20070300
• [Presentation] MICROBIAL IMPACTS ON THE MIGRATION OF ACTINIDES - Effects of exudates on the migration (2007)
  • Author(s): T. OHNUKI, T. OZAKI, T. YOSHIDA, T. NANKAWA, N. KOZAI, F. SAKAMOTO, Y. SUZUKI, A. J. FRANCIS
  • Organizer: 233rd American Chemical Society National Meeting & Exposition
  • Place of Presentation: Chicago
  • Year and Date: 20070300
• [Presentation] Microbial Impacts on the Migration of Actinides (2006)
  • Author(s): T. OHNUKI, T. OZAKI, T. NANKAWA, N. KOZAI, F. SAKAMOTO ,Y. SUZUKI, and A. J. FRANCIS
  • Organizer: International Symposium on Environmental Modeling and Radioecology
  • Place of Presentation: Rokkasho, Aomori, Japan
  • Year and Date: 20061000
• [Presentation] Interaction of Heavy Elements with Microorganisms (2006)
  • Author(s): T. Ohnuki, T. Yoshida, T. Ozaki, F. Sakamoto, N. Kozai, T. Nankawa, Y. Suzuki, A. J. Francis
  • Organizer: Plutonium future -The science 2006, Pacific Groove
  • Place of Presentation: CA, USA
  • Year and Date: 20060700
• [Presentation] Microbial impacts on the environmental behavior of actinides (2006)
  • Author(s): T. Ohnuki, T. Yoshida, T. Ozaki, F. Sakamoto, N. Kozai, T. Nankawa, Y. Suzuki, A. J. Francis
  • Organizer: 19th Genral Meetings of International Mineralogical Association
  • Place of Presentation: Kobe Japan
  • Year and Date: 20060600